##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631282.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	302392
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.79158840180957	34.0	31.0	34.0	31.0	34.0
2	32.96768763723908	34.0	33.0	34.0	31.0	34.0
3	33.084777375062835	34.0	33.0	34.0	31.0	34.0
4	36.419978041747136	37.0	37.0	37.0	35.0	37.0
5	36.388168337786716	37.0	37.0	37.0	35.0	37.0
6	36.420725416016296	37.0	37.0	37.0	35.0	37.0
7	36.41777890949496	37.0	37.0	37.0	35.0	37.0
8	36.4084168893357	37.0	37.0	37.0	35.0	37.0
9	38.25557885129236	39.0	39.0	39.0	37.0	39.0
10	38.21414256990926	39.0	39.0	39.0	37.0	39.0
11	38.26744093759094	39.0	39.0	39.0	37.0	39.0
12	38.048093203523905	39.0	39.0	39.0	37.0	39.0
13	38.1879117172412	39.0	39.0	39.0	37.0	39.0
14	39.69961176221593	41.0	40.0	41.0	37.0	41.0
15	39.699112410381225	41.0	40.0	41.0	38.0	41.0
16	39.66364850921982	41.0	40.0	41.0	37.0	41.0
17	39.656958517421096	41.0	40.0	41.0	37.0	41.0
18	39.64469628826159	41.0	40.0	41.0	37.0	41.0
19	39.64787097542263	41.0	40.0	41.0	37.0	41.0
20	39.60566417100981	41.0	40.0	41.0	37.0	41.0
21	39.58553467022937	41.0	40.0	41.0	37.0	41.0
22	39.53640307944654	41.0	39.0	41.0	37.0	41.0
23	39.47019431730998	41.0	39.0	41.0	37.0	41.0
24	39.43134077621101	41.0	39.0	41.0	37.0	41.0
25	39.42045424482129	41.0	39.0	41.0	37.0	41.0
26	39.34580941294743	41.0	39.0	41.0	36.0	41.0
27	39.27656485621313	41.0	39.0	41.0	36.0	41.0
28	39.19628495462843	41.0	39.0	41.0	36.0	41.0
29	39.13669673800894	41.0	39.0	41.0	35.0	41.0
30	39.08662596894098	40.0	39.0	41.0	35.0	41.0
31	39.0408906320273	40.0	39.0	41.0	35.0	41.0
32	38.9722347152041	40.0	39.0	41.0	35.0	41.0
33	38.880390354242174	40.0	38.0	41.0	35.0	41.0
34	38.841120135453316	40.0	38.0	41.0	35.0	41.0
35	38.73781052408794	40.0	38.0	41.0	35.0	41.0
36	38.66706129791793	40.0	38.0	41.0	35.0	41.0
37	38.626435223154054	40.0	38.0	41.0	35.0	41.0
38	38.53808301806926	40.0	38.0	41.0	35.0	41.0
39	38.463914389269554	40.0	38.0	41.0	35.0	41.0
40	38.36645149342575	40.0	38.0	41.0	34.0	41.0
41	38.28403529193894	40.0	38.0	41.0	34.0	41.0
42	38.17135373951692	40.0	37.0	41.0	34.0	41.0
43	37.229576179264	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	4.0
17	4.0
18	6.0
19	17.0
20	19.0
21	46.0
22	80.0
23	129.0
24	204.0
25	304.0
26	490.0
27	740.0
28	1033.0
29	1398.0
30	1902.0
31	2526.0
32	3395.0
33	4354.0
34	6626.0
35	9436.0
36	15081.0
37	28250.0
38	66247.0
39	160101.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.794782930765365	18.204515992486574	12.580359268763724	26.420341807984336
2	19.770364295351726	19.471745284266778	33.43937670308738	27.318513717294106
3	20.177782481018017	21.387470568004442	28.524233445329237	29.910513505648296
4	15.057938040688907	15.579446546204926	32.99723537659727	36.3653800365089
5	16.700507949945766	34.29918780920131	32.359652371755864	16.640651869097066
6	35.354440593666496	33.495925818143334	15.55728987539353	15.592343712796636
7	30.767348342548743	29.048056826900183	20.320643403264636	19.863951427286437
8	28.421056112595572	31.531257440673034	19.231328871134156	20.816357575597237
9	27.198470859017434	14.175308870605042	19.354678695203578	39.27154157517395
10	19.31433371253208	24.670626207042513	30.519987301251355	25.495052779174053
11	37.9398264504352	20.68870869599725	20.043519669832534	21.327945183735018
12	23.61272784994312	24.773142146617637	26.802627053625756	24.811502949813487
13	31.243551416704147	18.048427207068972	23.714251699780416	26.993769676446465
14	23.823712267520307	19.660903727611842	23.14809915606233	33.367284848805525
15	26.971282309055795	25.965634011481786	20.77601259292574	26.287071086536677
16	26.783115955448555	24.43814651181248	22.80252123072039	25.976216302018575
17	25.567144633455914	24.984457260774096	23.60346834572343	25.844929760046565
18	25.80491547395434	23.30749490727268	24.809188073758566	26.07840154501442
19	26.27615809942062	23.989060557157597	24.250972247943068	25.483809095478716
20	26.27318183020715	23.3891769623535	24.19806079525913	26.13958041218022
21	27.760985740363502	23.51318817958147	23.629593375486124	25.096232704568905
22	27.370102383660942	23.347509193364903	23.437127966348317	25.845260456625834
23	26.00399481467764	23.36172914627371	24.06941982592132	26.56485621312733
24	25.72720177782481	24.22650070107675	23.785351464324453	26.26094605677399
25	26.08600756633773	23.12362760919601	24.016839069816662	26.773525754649597
26	26.029127754702508	24.200375671314056	24.14448794941665	25.626008624566786
27	26.53806979020609	23.35313103521257	23.800232810391808	26.308566364189527
28	25.610465885340883	23.796925844599063	24.741395275007275	25.85121299505278
29	25.479179343368873	23.87166327151512	24.798275086642505	25.850882298473504
30	24.87102833408291	24.265853594010423	24.884256197253897	25.97886187465277
31	25.918013704066244	23.757242255086116	23.93085795920527	26.39388608164237
32	25.15939575121035	23.374295616286144	24.420288896531655	27.04601973597185
33	24.732797163946135	23.02342654567581	25.21892113547978	27.024855154898276
34	26.001018545464166	22.668589116114184	25.08531971745284	26.245072620968806
35	25.93785549882272	22.800867747824014	25.367403899574064	25.8938728537792
36	24.602172015132677	23.111391835762852	25.57673483425487	26.7097013148496
37	24.835313103521255	21.997275060186777	26.000357152305615	27.167054683986347
38	24.000965634011482	21.65897245958888	27.301648191751106	27.038413714648534
39	24.358448636207306	21.362337627979578	27.468980660864045	26.810233074949075
40	23.72814095610995	22.01281779941268	28.14029471679145	26.118746527685914
41	22.791608243604326	21.994960184131855	28.875763909098122	26.337667663165693
42	23.120982036561813	22.440077779835445	28.620466149898142	25.818474033704597
43	22.20825947776396	21.491309293896663	28.793420460858755	27.507010767480622
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	20.0
1	24.5
2	29.0
3	67.5
4	106.0
5	106.0
6	137.5
7	169.0
8	156.0
9	143.0
10	214.5
11	286.0
12	286.0
13	465.5
14	645.0
15	913.5
16	1182.0
17	1097.5
18	1013.0
19	1013.0
20	1193.0
21	1373.0
22	1243.0
23	1113.0
24	1301.0
25	1489.0
26	1489.0
27	1815.5
28	2142.0
29	2801.5
30	3461.0
31	4251.0
32	5041.0
33	5041.0
34	6259.5
35	7478.0
36	9122.5
37	10767.0
38	12349.5
39	13932.0
40	13932.0
41	15119.0
42	16306.0
43	17390.5
44	18475.0
45	19437.0
46	20399.0
47	20399.0
48	21637.5
49	22876.0
50	23215.0
51	23554.0
52	23788.5
53	24023.0
54	24023.0
55	23085.5
56	22148.0
57	21382.0
58	20616.0
59	19954.5
60	19293.0
61	19293.0
62	17984.0
63	16675.0
64	15268.0
65	13861.0
66	12423.5
67	10986.0
68	10986.0
69	9377.0
70	7768.0
71	6768.0
72	5768.0
73	4521.0
74	3274.0
75	3274.0
76	2627.0
77	1980.0
78	1689.0
79	1398.0
80	1220.0
81	1042.0
82	1042.0
83	827.0
84	612.0
85	517.5
86	423.0
87	366.5
88	310.0
89	310.0
90	240.0
91	170.0
92	102.0
93	34.0
94	21.5
95	9.0
96	9.0
97	6.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	302392.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.9948243452602
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.33292967585353	61.42171685902403
2	5.085676104611412	6.9159930681988016
3	1.5069385436388694	3.073920647214814
4	0.753643403538854	2.049754033703537
5	0.42724632445049887	1.4525269391584859
6	0.30327346831566365	1.237261572402083
7	0.22826428435567367	1.086455294334239
8	0.18149857926127122	0.9872771212627538
9	0.14653709923216338	0.8967387890119419
>10	0.890041116831782	11.638198938077595
>50	0.10247387790225737	4.717460177936856
>100	0.04098955116090275	4.345849867113287
>500	4.8797084715360416E-4	0.1768466925615653
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	533	0.17626127675335326	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	473	0.15641948199687822	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	413	0.13657768724040317	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	345	0.11409031984973148	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	3.30696579274584E-4	0.0
14	0.0	0.0	0.0	6.61393158549168E-4	0.0
15	0.0	0.0	0.0	9.92089737823752E-4	0.0
16	0.0	0.0	0.0	9.92089737823752E-4	0.0
17	0.0	0.0	0.0	9.92089737823752E-4	0.0
18	0.0	0.0	0.0	0.001322786317098336	0.0
19	0.0	0.0	0.0	0.001322786317098336	0.0
20	0.0	0.0	0.0	0.001984179475647504	0.0
21	0.0	0.0	0.0	0.002645572634196672	0.0
22	0.0	0.0	0.0	0.004299055530569592	0.0
23	0.0	0.0	0.0	0.006613931585491679	0.0
24	0.0	0.0	0.0	0.012235773433159607	0.0
25	0.0	0.0	0.0	0.0185190084393767	0.0
26	0.0	0.0	0.0	0.02744781607979047	0.0
27	0.0	3.30696579274584E-4	0.0	0.07506812349533057	0.0
28	0.0	3.30696579274584E-4	0.0	0.2619116907854705	0.0
29	0.0	3.30696579274584E-4	0.0	0.5208471123574697	0.0
30	0.0	3.30696579274584E-4	0.0	0.8707240932299797	0.0
31	0.0	3.30696579274584E-4	0.0	1.6832455885076325	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTATGT	25	0.005490018	29.6	8
GCTCTAA	25	0.005490018	29.6	1
TAGGGAT	25	0.005490018	29.6	5
CCGCTTA	40	5.9258804E-5	27.75	25
CGCTTAT	40	5.9258804E-5	27.75	26
GCAGTCG	100	5.293259E-10	22.2	9
TCGGTGA	110	7.2759576E-11	21.863636	13
GGTATCA	365	0.0	21.79452	1
AGTCGGT	105	9.749783E-10	21.142857	11
CCGGAAC	80	6.9252746E-7	20.8125	13
CAGTCGG	110	1.7389539E-9	20.181818	10
GTCCTAT	90	2.1415617E-6	18.5	1
TATGGGC	160	1.8189894E-12	18.5	4
GGATACC	100	2.8575596E-7	18.5	25
TGGATAC	100	2.8575596E-7	18.5	24
TATACAC	180	0.0	18.5	37
CTCTATG	150	1.2732926E-11	18.499998	1
TCTATGG	155	2.0008883E-11	17.903225	2
ACCATAC	105	4.768426E-7	17.619047	1
CGGAACC	95	3.588637E-6	17.526316	14
>>END_MODULE