FastQCFastQC Report
Fri 10 Feb 2017
ERR1631281.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631281.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103512
Sequences flagged as poor quality0
Sequence length43
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA2170.20963752994821858No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC1800.17389288198469743No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC1660.16036788005255428No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC1600.15457145065306438No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC1490.1439446634206662No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC1430.1381482340211763No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC1380.13331787618826801No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT1290.12462323208903316No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG1220.11786073112296158No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC1120.10820001545714508No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG1110.10723394389056341No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT1060.10240358605765516No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC1040.10047144292449185No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA801.8189894E-1230.06251
GCATTTG250.0054735529.624
GTTCAAA451.3118822E-424.6666681
TGCATCA400.001919402723.12514
TTCAAAG502.6791522E-422.22
TCAAAGC603.6842954E-521.5833323
GTATCAA1151.3096724E-1020.9130442
CCTTGGA450.003802684420.5555571
AGCCGCC609.159639E-418.517
ATGTGGT500.00699276918.57
CTGCATC500.00699276918.513
ATCACAG650.00156684117.07692336
CTTATAC1950.017.07692337
CAAAGCA650.00156684117.0769234
TCTTATA3450.016.62318837
CCAGAGC700.002571185815.85714313
CAGGACA700.002571185815.8571434
TTTCTCA700.002571185815.85714319
AAAGCAG700.002571185815.8571435
CCAGGAC750.004070951614.7999993
Produced by FastQC (version 0.11.5)