##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631281.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	103512
Sequences flagged as poor quality	0
Sequence length	43
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.66942963134709	34.0	31.0	34.0	31.0	34.0
2	32.865522837931834	34.0	31.0	34.0	31.0	34.0
3	32.977104103872016	34.0	33.0	34.0	31.0	34.0
4	36.35470283638612	37.0	37.0	37.0	35.0	37.0
5	36.315634902233555	37.0	37.0	37.0	35.0	37.0
6	36.35902117628874	37.0	37.0	37.0	35.0	37.0
7	36.343786227683744	37.0	37.0	37.0	35.0	37.0
8	36.33699474457068	37.0	37.0	37.0	35.0	37.0
9	38.167178684596955	39.0	39.0	39.0	37.0	39.0
10	38.095158049308296	39.0	38.0	39.0	37.0	39.0
11	38.16379743411392	39.0	38.0	39.0	37.0	39.0
12	37.922356828193834	39.0	38.0	39.0	35.0	39.0
13	38.062379241054174	39.0	38.0	39.0	37.0	39.0
14	39.538923023417574	41.0	39.0	41.0	37.0	41.0
15	39.54107736301105	41.0	39.0	41.0	37.0	41.0
16	39.51070407295772	41.0	39.0	41.0	37.0	41.0
17	39.49702449957493	41.0	39.0	41.0	37.0	41.0
18	39.44210333101476	41.0	39.0	41.0	37.0	41.0
19	39.45030527861504	41.0	39.0	41.0	37.0	41.0
20	39.43007574001082	41.0	39.0	41.0	37.0	41.0
21	39.390138341448335	41.0	39.0	41.0	36.0	41.0
22	39.329449725635676	40.0	39.0	41.0	36.0	41.0
23	39.238252569750365	40.0	39.0	41.0	36.0	41.0
24	39.195677795811115	40.0	39.0	41.0	36.0	41.0
25	39.17711956101708	40.0	39.0	41.0	36.0	41.0
26	39.07897635056805	40.0	39.0	41.0	35.0	41.0
27	39.00581575083082	40.0	39.0	41.0	35.0	41.0
28	38.88796468042353	40.0	39.0	41.0	35.0	41.0
29	38.80213888244841	40.0	38.0	41.0	35.0	41.0
30	38.74140196305742	40.0	38.0	41.0	35.0	41.0
31	38.7181776025968	40.0	38.0	41.0	35.0	41.0
32	38.629627482803926	40.0	38.0	41.0	35.0	41.0
33	38.525803771543394	40.0	38.0	41.0	35.0	41.0
34	38.47195494242214	40.0	38.0	41.0	34.0	41.0
35	38.3385984233712	40.0	38.0	41.0	34.0	41.0
36	38.27768761109823	40.0	38.0	41.0	34.0	41.0
37	38.21470940567277	40.0	38.0	41.0	34.0	41.0
38	38.1367281088183	40.0	37.0	41.0	34.0	41.0
39	38.04922134631734	40.0	37.0	41.0	34.0	41.0
40	37.94125318803617	40.0	37.0	41.0	33.0	41.0
41	37.86609282015612	40.0	37.0	41.0	33.0	41.0
42	37.750057964293994	40.0	36.0	41.0	33.0	41.0
43	36.721095138727875	39.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	1.0
18	1.0
19	7.0
20	10.0
21	24.0
22	32.0
23	73.0
24	107.0
25	133.0
26	206.0
27	297.0
28	404.0
29	529.0
30	744.0
31	999.0
32	1302.0
33	1783.0
34	2708.0
35	4166.0
36	6417.0
37	11010.0
38	26028.0
39	46530.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.68962052708865	16.959386351340907	11.688499884071412	27.662493237499035
2	22.46696035242291	18.165043666434812	30.550081150011593	28.817914831130693
3	22.185833526547647	19.00745807249401	26.722505603215087	32.08420279774325
4	16.727529175361312	14.55773243681892	30.05158822165546	38.663150166164314
5	18.64904552129222	32.43005641857949	30.66407759486823	18.256820465260066
6	38.7636216090888	30.71914367416338	13.879550197078599	16.637684519669214
7	32.99907257129608	27.075121725017386	17.982456140350877	21.94334956333565
8	30.18297395471056	29.722157817451116	17.301375685910813	22.793492541927506
9	27.72625396089342	13.510510858644409	17.39218641316949	41.37104876729268
10	20.72416724630961	23.030180075740013	28.121377231625317	28.124275446325065
11	40.736339748048536	19.224824174974884	17.734175747739393	22.304660329237187
12	26.086830512404358	22.000347785763967	23.920898060128295	27.991923641703377
13	33.18842259834609	15.996212999459	21.857369193909886	28.95799520828503
14	25.4579179225597	18.23653296236185	20.413092201870313	35.89245691320813
15	29.740513177216165	23.52094443156349	18.272277610325375	28.466264780894967
16	29.696073885153414	22.074735296390756	19.571643867377695	28.65754695107813
17	28.03732900533272	22.64375144910735	20.73286189040884	28.58605765515109
18	28.235373676481956	20.045018935002705	22.378081768297395	29.341525620217944
19	28.42858798979828	21.80616740088106	20.768606538372364	28.996638070948293
20	28.70585052940722	20.63239044748435	21.14151016307288	29.520248860035554
21	31.246618749516962	20.893229770461396	20.61017080145297	27.24998067856867
22	31.334531262075892	20.7087101012443	19.678877811268258	28.277880825411543
23	28.658513022644716	20.852654764664965	21.025581575083084	29.463250637607235
24	28.344539763505683	21.829353118479016	20.927042275291754	28.899064842723547
25	28.658513022644716	20.677795811113686	20.91448334492619	29.749207821315405
26	29.02851843264549	21.770422752917536	21.437128062446867	27.763930751990106
27	29.621686374526625	20.545444006492	21.289319112759873	28.5435505062215
28	28.341641548805935	20.660406522915217	22.096954942422133	28.900996985856715
29	27.05966457995208	21.139578019939716	22.75388360769766	29.046873792410544
30	26.67130381018626	22.297897828271118	22.723935389133626	28.306862972408997
31	28.759950537135794	21.91340134477162	20.818842259834607	28.507805858257978
32	27.229693175670455	20.94153334879048	21.90567277223897	29.923100703300097
33	27.22003246000464	20.98307442615349	22.201290671612952	29.59560244222892
34	28.363861194837312	20.710642244377464	22.481451425921634	28.44404513486359
35	28.124275446325065	19.90783677254811	23.770190895741557	28.19769688538527
36	26.536053790864827	21.160831594404513	23.01279078754154	29.29032382718912
37	26.61913594559085	19.264433109204727	24.991305355900764	29.125125589303657
38	25.802805471829355	18.526354432336348	26.339941262848754	29.330898832985547
39	25.235721462245923	18.383375840482262	27.545598577942656	28.83530411932916
40	24.70051781435969	18.467424066774868	27.93878970554139	28.89326841332406
41	23.188615812659403	18.951425921632275	29.709598887085555	28.15035937862277
42	23.381830125975732	19.204536672076667	29.35601669371667	28.057616508230932
43	21.277726253960893	18.394002627714663	30.02067393152485	30.3075971867996
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	1.0
2	1.0
3	1.0
4	1.0
5	1.0
6	2.0
7	3.0
8	5.5
9	8.0
10	12.5
11	17.0
12	17.0
13	24.5
14	32.0
15	49.5
16	67.0
17	60.0
18	53.0
19	53.0
20	65.0
21	77.0
22	79.0
23	81.0
24	104.0
25	127.0
26	127.0
27	178.0
28	229.0
29	305.5
30	382.0
31	542.0
32	702.0
33	702.0
34	933.0
35	1164.0
36	1575.0
37	1986.0
38	2354.0
39	2722.0
40	2722.0
41	3113.5
42	3505.0
43	3984.0
44	4463.0
45	4943.0
46	5423.0
47	5423.0
48	5940.5
49	6458.0
50	6975.5
51	7493.0
52	8349.0
53	9205.0
54	9205.0
55	9067.5
56	8930.0
57	8856.0
58	8782.0
59	8709.5
60	8637.0
61	8637.0
62	8378.0
63	8119.0
64	7575.5
65	7032.0
66	6240.0
67	5448.0
68	5448.0
69	4666.0
70	3884.0
71	3417.0
72	2950.0
73	2450.5
74	1951.0
75	1951.0
76	1747.5
77	1544.0
78	1276.0
79	1008.0
80	725.0
81	442.0
82	442.0
83	361.5
84	281.0
85	213.0
86	145.0
87	113.5
88	82.0
89	82.0
90	68.5
91	55.0
92	34.5
93	14.0
94	8.5
95	3.0
96	3.0
97	3.5
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	103512.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.88619676945669
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.86771383905308	57.66287966612567
2	5.063649222065063	6.571218795888399
3	1.7866448298965234	3.4778576396939487
4	1.0258319064989205	2.6624932374990338
5	0.620859078389042	2.0142592163227455
6	0.3677510608203678	1.4317180616740088
7	0.34690687113824165	1.575662725094675
8	0.22928608650338717	1.1902001700285956
9	0.1846199657559741	1.078135868305124
>10	1.4144271570014144	17.367068552438365
>50	0.07146579319586094	3.0962593708941957
>100	0.020844189682126108	1.8722466960352422
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	217	0.20963752994821858	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	180	0.17389288198469743	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	166	0.16036788005255428	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	160	0.15457145065306438	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	149	0.1439446634206662	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	143	0.1381482340211763	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	138	0.13331787618826801	No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT	129	0.12462323208903316	No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG	122	0.11786073112296158	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	112	0.10820001545714508	No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG	111	0.10723394389056341	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	106	0.10240358605765516	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC	104	0.10047144292449185	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0019321431331633047	0.0
18	0.0	0.0	0.0	0.0019321431331633047	0.0
19	0.0	0.0	0.0	0.0019321431331633047	0.0
20	0.0	0.0	0.0	0.0019321431331633047	0.0
21	0.0	0.0	0.0	0.0019321431331633047	0.0
22	0.0	0.0	0.0	0.002898214699744957	0.0
23	0.0	0.0	0.0	0.002898214699744957	0.0
24	0.0	0.0	0.0	0.0038642862663266094	0.0
25	0.0	0.0	0.0	0.005796429399489914	0.0
26	0.0	0.0	0.0	0.012558930365561482	0.0
27	0.0	0.0	0.0	0.05313393616199088	0.0
28	0.0	0.0	0.0	0.23861967694566813	0.0
29	0.0	0.0	0.0	0.47530721075817295	0.0
30	0.0	0.0	0.0	0.7989411855630265	0.0
31	0.0	0.0	0.0	1.5698662956951852	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	80	1.8189894E-12	30.0625	1
GCATTTG	25	0.00547355	29.6	24
GTTCAAA	45	1.3118822E-4	24.666668	1
TGCATCA	40	0.0019194027	23.125	14
TTCAAAG	50	2.6791522E-4	22.2	2
TCAAAGC	60	3.6842954E-5	21.583332	3
GTATCAA	115	1.3096724E-10	20.913044	2
CCTTGGA	45	0.0038026844	20.555557	1
AGCCGCC	60	9.159639E-4	18.5	17
ATGTGGT	50	0.006992769	18.5	7
CTGCATC	50	0.006992769	18.5	13
ATCACAG	65	0.001566841	17.076923	36
CTTATAC	195	0.0	17.076923	37
CAAAGCA	65	0.001566841	17.076923	4
TCTTATA	345	0.0	16.623188	37
CCAGAGC	70	0.0025711858	15.857143	13
CAGGACA	70	0.0025711858	15.857143	4
TTTCTCA	70	0.0025711858	15.857143	19
AAAGCAG	70	0.0025711858	15.857143	5
CCAGGAC	75	0.0040709516	14.799999	3
>>END_MODULE