FastQCFastQC Report
Fri 10 Feb 2017
ERR1631280.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631280.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154524
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7970.5157774844037172No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6840.44264968548574973No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA6430.4161165903031244No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT5510.3565789133079651No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT4320.2795682224120525No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC3770.24397504594755506No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT3730.24138645129559164No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT3730.24138645129559164No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA3650.23620926199166473No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC3580.2316792213507287No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT3560.23038492402474697No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA3150.2038518288421216No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA2990.19349745023426781No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACCTGTCT2470.15984571975874298No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT2190.14172555719499882No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2180.14107840853200798No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA2140.13848981388004453No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT2100.13590121922808107No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT2080.13460692190209936No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA1970.12748828660919986No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT1830.1184282053273278No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA1800.11648675933835521No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCT1560.10095519142657451No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCTG200.001837009637.035
AGTTCCC352.3750485E-531.7142875
GGACTGC250.005481811729.66
ATATGCT250.005481811729.635
CTTGGAC250.005481811729.63
GTCCTAA453.9788083E-628.7777791
AAGGTAG453.9788083E-628.7777796
TAAGGTA453.9788083E-628.7777795
TTATTGC405.9074155E-527.75000229
AGGTAGC509.024765E-625.9000027
GGTAGCG509.024765E-625.9000028
TATTGCT451.3159837E-424.66666830
ATGCCCC901.4006218E-1024.66666837
ATACACA1250.023.6837
ATTCCTT551.888876E-523.54545417
AATTCCT551.888876E-523.54545416
TTAGGAC502.6874608E-422.23
CGAAATT502.6874608E-422.213
GCGAAAT502.6874608E-422.212
CTTAGGA502.6874608E-422.22
Produced by FastQC (version 0.11.5)