FastQCFastQC Report
Fri 10 Feb 2017
ERR1631279.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631279.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1206946
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA18270.1513737979992477No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC16390.13579729333375312No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC13810.11442102629280845No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC12580.10423001526166041No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGTCG405.9403723E-527.7500025
GTCTAGA2400.023.1249981
TTATTCT502.7022112E-422.24
TAGAACT2700.021.9259264
ATACACA6450.021.7984537
GGTATCA8150.021.110431
ACGGAGT1950.020.87179626
TATGGTA450.003825917420.5555552
ACTGATC2250.019.7333348
TACACAT2450.019.63265237
AACGGAG2200.019.34090825
CTAGAAC3000.019.1166673
ACAACGG2150.018.93023323
CAACGGA2150.018.93023324
ATTACAA2150.018.93023320
TTACACT3150.018.7936524
GTCATAT1205.173206E-918.51
CACCGTA500.007035081718.57
TCGACCT500.007035081718.526
GTATCAA8850.018.395482
Produced by FastQC (version 0.11.5)