##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631279.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1206946
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.86608928651323	34.0	33.0	34.0	31.0	34.0
2	33.04651326571363	34.0	33.0	34.0	31.0	34.0
3	33.16434040959579	34.0	33.0	34.0	31.0	34.0
4	36.47117186684408	37.0	37.0	37.0	35.0	37.0
5	36.45192742674486	37.0	37.0	37.0	35.0	37.0
6	36.4984166648715	37.0	37.0	37.0	35.0	37.0
7	36.488566182745544	37.0	37.0	37.0	35.0	37.0
8	36.46834406841731	37.0	37.0	37.0	35.0	37.0
9	38.32703700082688	39.0	39.0	39.0	37.0	39.0
10	38.29405043804777	39.0	39.0	39.0	37.0	39.0
11	38.35402329515985	39.0	39.0	39.0	37.0	39.0
12	38.134947213877005	39.0	39.0	39.0	37.0	39.0
13	38.26253618637453	39.0	39.0	39.0	37.0	39.0
14	39.80323229042558	41.0	40.0	41.0	38.0	41.0
15	39.81598016812683	41.0	40.0	41.0	38.0	41.0
16	39.81618067419752	41.0	40.0	41.0	38.0	41.0
17	39.80271694011165	41.0	40.0	41.0	38.0	41.0
18	39.78696064281252	41.0	40.0	41.0	38.0	41.0
19	39.793985812124156	41.0	40.0	41.0	38.0	41.0
20	39.77133111174816	41.0	40.0	41.0	38.0	41.0
21	39.73531044470921	41.0	40.0	41.0	38.0	41.0
22	39.69777769676522	41.0	40.0	41.0	38.0	41.0
23	39.648703421694094	41.0	40.0	41.0	37.0	41.0
24	39.61761752389916	41.0	40.0	41.0	37.0	41.0
25	39.593207152598374	41.0	40.0	41.0	37.0	41.0
26	39.550737978335405	41.0	39.0	41.0	37.0	41.0
27	39.47895100526453	41.0	39.0	41.0	37.0	41.0
28	39.40914755092606	41.0	39.0	41.0	37.0	41.0
29	39.35774508552993	41.0	39.0	41.0	36.0	41.0
30	39.31320291048647	41.0	39.0	41.0	36.0	41.0
31	39.289793412464185	41.0	39.0	41.0	36.0	41.0
32	39.21752588765363	41.0	39.0	41.0	36.0	41.0
33	39.15531846495204	41.0	39.0	41.0	35.0	41.0
34	39.11482286697168	40.0	39.0	41.0	35.0	41.0
35	39.04479570751301	40.0	39.0	41.0	35.0	41.0
36	38.99996685850071	40.0	39.0	41.0	35.0	41.0
37	38.957346061878496	40.0	39.0	41.0	35.0	41.0
38	38.8857554521909	40.0	38.0	41.0	35.0	41.0
39	38.82799644723128	40.0	38.0	41.0	35.0	41.0
40	38.758486295161504	40.0	38.0	41.0	35.0	41.0
41	38.69656554642876	40.0	38.0	41.0	35.0	41.0
42	38.611278383622796	40.0	38.0	41.0	35.0	41.0
43	37.69154792343651	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	1.0
13	0.0
14	1.0
15	1.0
16	4.0
17	6.0
18	14.0
19	32.0
20	61.0
21	112.0
22	217.0
23	373.0
24	639.0
25	939.0
26	1482.0
27	2052.0
28	3075.0
29	4163.0
30	5579.0
31	7739.0
32	10409.0
33	14419.0
34	21654.0
35	32447.0
36	53635.0
37	100540.0
38	257021.0
39	690329.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.440717314610595	16.895370629671916	11.829692463457354	27.834219592260133
2	20.885772851478027	18.522949659719657	32.47850359502414	28.112773893778183
3	21.43492749468493	19.35786688053981	27.11968886760468	32.087516757170576
4	16.371237818427666	14.31895047500054	30.56309064365763	38.74672106291416
5	17.96749813164798	33.09717253298822	31.249533947666258	17.685795387697546
6	38.423757152349815	31.028397293665165	14.269486787312772	16.278358766672245
7	32.65622488495757	27.000959446404394	18.98452789105726	21.35828777758077
8	28.70202975112391	30.699136498236047	18.595446689412782	22.003387061227265
9	27.79478120810707	13.693156114689472	18.001799583411355	40.5102630937921
10	19.545447766511508	23.966855186561784	29.8022446737468	26.685452373179906
11	39.616022589245915	19.597811335387004	18.809955043556215	21.97621103181087
12	25.031691558694426	22.927786330125787	25.548201825102367	26.49232028607742
13	32.684229451856176	16.859577810440566	22.79604887045485	27.66014386724841
14	25.037822736062758	18.337605824949915	21.370550132317437	35.25402130666989
15	27.850376073163176	25.016197907777148	19.362423836691946	27.771002182367727
16	28.286435350048798	22.999620529833148	21.03292110831802	27.681023011800033
17	27.448618248040923	23.37917355043225	21.85980151556076	27.312406685966067
18	27.83811371842651	21.513887116739276	22.95363669957065	27.694362465263566
19	27.428899055964393	22.50887777912185	22.32817375425247	27.73404941066129
20	27.687319896664803	21.859304393071437	22.551298898210856	27.9020768120529
21	28.86550019636338	22.021863447080484	22.75661048630179	26.356025870254346
22	29.32674701270811	21.645624576410214	21.379829752118155	27.647798658763524
23	27.575053067825735	21.885817592502065	22.538373713488426	28.00075562618377
24	27.40536859146971	22.430829548297933	22.07845255711523	28.085349303117123
25	27.77108503611595	22.10977127394266	22.211018554268378	27.90812513567301
26	28.059913202413362	22.437789263148474	22.011838143545777	27.490459390892386
27	28.40110493758627	21.63468788164508	22.542019278410137	27.422187902358512
28	27.075527819803042	22.207207281850224	22.987855297585806	27.729409600760928
29	26.79556500456524	22.16172057407705	22.943777103532387	28.098937317825325
30	25.916155320950562	22.630507081509858	24.28012520858431	27.173212388955264
31	27.84971324317741	21.87927214639263	22.410198964990975	27.860815645438986
32	26.722736559879234	21.406674366541665	23.280329028804932	28.590260044774162
33	26.806584553078594	21.35489077390372	23.27063514026311	28.56788953275457
34	27.561796468110423	20.788171136074023	23.86983344739533	27.780198948420225
35	26.599615890023248	20.44026824729524	24.80906353722536	28.15105232545615
36	25.43742636373127	20.306873712659886	25.84498395122897	28.410715972379876
37	26.462824351710847	19.30359767545524	26.059409451624184	28.17416852120973
38	24.811632003420204	18.58011874599195	27.88451181743011	28.723737433157737
39	25.423175519037304	18.748394708628226	28.274090141563914	27.554339630770556
40	24.139605251601978	19.676936664937784	28.105897032675863	28.07756105078438
41	23.35837725962885	19.600462655330062	29.74598697870493	27.295173106336158
42	23.248347481991736	19.853166587403248	29.27811186250255	27.620374068102464
43	21.28620501662875	19.809751223335592	29.495520097833705	29.40852366220195
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	21.0
1	18.0
2	15.0
3	36.5
4	58.0
5	58.0
6	72.5
7	87.0
8	90.5
9	94.0
10	122.0
11	150.0
12	150.0
13	225.0
14	300.0
15	473.5
16	647.0
17	628.0
18	609.0
19	609.0
20	805.0
21	1001.0
22	1032.5
23	1064.0
24	1383.5
25	1703.0
26	1703.0
27	2242.0
28	2781.0
29	3896.5
30	5012.0
31	6531.5
32	8051.0
33	8051.0
34	11494.5
35	14938.0
36	19782.5
37	24627.0
38	30740.5
39	36854.0
40	36854.0
41	41839.0
42	46824.0
43	52228.0
44	57632.0
45	67214.5
46	76797.0
47	76797.0
48	83868.5
49	90940.0
50	96075.0
51	101210.0
52	107652.0
53	114094.0
54	114094.0
55	112912.0
56	111730.0
57	110010.0
58	108290.0
59	104516.5
60	100743.0
61	100743.0
62	95179.0
63	89615.0
64	81374.5
65	73134.0
66	64360.0
67	55586.0
68	55586.0
69	45172.5
70	34759.0
71	28774.5
72	22790.0
73	16303.5
74	9817.0
75	9817.0
76	7482.5
77	5148.0
78	4245.5
79	3343.0
80	2971.0
81	2599.0
82	2599.0
83	2068.0
84	1537.0
85	1314.5
86	1092.0
87	891.5
88	691.0
89	691.0
90	561.5
91	432.0
92	253.5
93	75.0
94	56.5
95	38.0
96	38.0
97	26.5
98	15.0
99	9.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1206946.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.02409738703738
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.90035133286607	31.960872864463415
2	7.775213985808831	6.0684141557451765
3	2.9475259439512436	3.450736184627178
4	1.6418856481157686	2.562924217217949
5	1.0027340289059705	1.9565395198661473
6	0.6859593239228913	1.606136607618793
7	0.5098555697192219	1.3927657384232437
8	0.40194205062940475	1.254834058216592
9	0.3091983861291663	1.0859569138997434
>10	2.240553170998593	17.593198350420238
>50	0.3304961820684005	9.110746862992842
>100	0.2393012907553059	17.682206030222538
>500	0.013270733428704978	3.4043822085182662
>1k	0.0017123527004780617	0.8702862877677899
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1827	0.1513737979992477	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	1639	0.13579729333375312	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	1381	0.11442102629280845	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	1258	0.10423001526166041	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	8.285374822071576E-5	0.0	0.0	0.0
6	0.0	8.285374822071576E-5	0.0	0.0	0.0
7	0.0	8.285374822071576E-5	0.0	0.0	0.0
8	0.0	8.285374822071576E-5	0.0	0.0	0.0
9	0.0	1.6570749644143152E-4	0.0	0.0	0.0
10	0.0	1.6570749644143152E-4	0.0	0.0	0.0
11	0.0	2.4856124466214727E-4	0.0	0.0	0.0
12	0.0	2.4856124466214727E-4	0.0	0.0	0.0
13	0.0	2.4856124466214727E-4	0.0	0.0	0.0
14	0.0	2.4856124466214727E-4	0.0	0.0	0.0
15	0.0	2.4856124466214727E-4	0.0	0.0	0.0
16	0.0	2.4856124466214727E-4	0.0	3.3141499288286304E-4	0.0
17	0.0	2.4856124466214727E-4	0.0	4.971224893242945E-4	0.0
18	0.0	2.4856124466214727E-4	0.0	7.456837339864419E-4	0.0
19	0.0	2.4856124466214727E-4	0.0	7.456837339864419E-4	0.0
20	0.0	2.4856124466214727E-4	0.0	0.0012428062233107364	0.0
21	0.0	2.4856124466214727E-4	0.0	0.0018227824608557466	0.0
22	0.0	2.4856124466214727E-4	0.0	0.002402758698400757	0.0
23	0.0	2.4856124466214727E-4	0.0	0.004805517396801514	0.0
24	0.0	2.4856124466214727E-4	0.0	0.010273864779368754	0.0
25	0.0	2.4856124466214727E-4	0.0	0.015079382176170268	0.0
26	0.0	2.4856124466214727E-4	0.0	0.028418835639705504	0.0
27	0.0	2.4856124466214727E-4	0.0	0.10638421271539904	0.0
28	0.0	3.3141499288286304E-4	0.0	0.39347245030017913	8.285374822071576E-5
29	0.0	4.1426874110357876E-4	0.0	0.7721969334170709	8.285374822071576E-5
30	0.0	4.1426874110357876E-4	0.0	1.288127223587468	8.285374822071576E-5
31	0.0	4.1426874110357876E-4	0.0	2.448576821166813	8.285374822071576E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGTCG	40	5.9403723E-5	27.750002	5
GTCTAGA	240	0.0	23.124998	1
TTATTCT	50	2.7022112E-4	22.2	4
TAGAACT	270	0.0	21.925926	4
ATACACA	645	0.0	21.79845	37
GGTATCA	815	0.0	21.11043	1
ACGGAGT	195	0.0	20.871796	26
TATGGTA	45	0.0038259174	20.555555	2
ACTGATC	225	0.0	19.733334	8
TACACAT	245	0.0	19.632652	37
AACGGAG	220	0.0	19.340908	25
CTAGAAC	300	0.0	19.116667	3
ACAACGG	215	0.0	18.930233	23
CAACGGA	215	0.0	18.930233	24
ATTACAA	215	0.0	18.930233	20
TTACACT	315	0.0	18.793652	4
GTCATAT	120	5.173206E-9	18.5	1
CACCGTA	50	0.0070350817	18.5	7
TCGACCT	50	0.0070350817	18.5	26
GTATCAA	885	0.0	18.39548	2
>>END_MODULE