FastQCFastQC Report
Fri 10 Feb 2017
ERR1631278.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631278.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences667718
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12190.1825620995689797No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12120.18151375281181575No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT8740.13089358082304206No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA7380.11052570096957098No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTTC250.005494714729.620
GGTATCA8450.024.9585781
GTATGCC551.9011106E-523.54545411
AGCGTCA652.6800517E-622.769233
GCAGTCG2050.022.5609769
TTACGGA603.723277E-521.58333237
ACCCGAT603.723277E-521.5833326
ATTAGAC705.096639E-621.1428593
TCGGTGA2200.021.02272813
TAAGTTA450.003824154820.5555555
TGTCGTA450.003824154820.55555530
GGACTAT555.1399914E-420.1818186
AAGATAC555.1399914E-420.18181826
GCTTACA656.896595E-519.9230772
CGGTGAT2350.019.68085114
TCTAGCG851.2437085E-619.58823628
CTAGCGG851.2437085E-619.58823629
CGCGGTT951.6734884E-719.47368410
CAGTCGG2300.019.30434810
GGCAGTC2350.018.8936188
Produced by FastQC (version 0.11.5)