##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631278.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	667718
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.661470860453065	34.0	31.0	34.0	31.0	34.0
2	32.85370920059067	34.0	31.0	34.0	31.0	34.0
3	32.965958683156664	34.0	33.0	34.0	31.0	34.0
4	36.346481598519134	37.0	37.0	37.0	35.0	37.0
5	36.298038992508815	37.0	37.0	37.0	35.0	37.0
6	36.34776058156287	37.0	37.0	37.0	35.0	37.0
7	36.349744952210365	37.0	37.0	37.0	35.0	37.0
8	36.33815622762903	37.0	37.0	37.0	35.0	37.0
9	38.16115186351124	39.0	38.0	39.0	37.0	39.0
10	38.096611144225555	39.0	38.0	39.0	37.0	39.0
11	38.16078194686979	39.0	38.0	39.0	37.0	39.0
12	37.94289205922261	39.0	38.0	39.0	35.0	39.0
13	38.08281190562483	39.0	38.0	39.0	37.0	39.0
14	39.548189505150376	41.0	39.0	41.0	37.0	41.0
15	39.53617545131328	41.0	39.0	41.0	37.0	41.0
16	39.50700894689076	41.0	39.0	41.0	37.0	41.0
17	39.49610614061625	41.0	39.0	41.0	37.0	41.0
18	39.48856702979401	41.0	39.0	41.0	37.0	41.0
19	39.50567005831803	41.0	39.0	41.0	37.0	41.0
20	39.468684384725286	41.0	39.0	41.0	37.0	41.0
21	39.449754237567355	41.0	39.0	41.0	37.0	41.0
22	39.39910860572876	41.0	39.0	41.0	37.0	41.0
23	39.34209052324484	40.0	39.0	41.0	36.0	41.0
24	39.324744877328456	41.0	39.0	41.0	36.0	41.0
25	39.31294798103391	41.0	39.0	41.0	36.0	41.0
26	39.227801257417056	40.0	39.0	41.0	36.0	41.0
27	39.167331118825615	40.0	39.0	41.0	36.0	41.0
28	39.092874536855376	40.0	39.0	41.0	36.0	41.0
29	39.03744544852767	40.0	39.0	41.0	35.0	41.0
30	38.97605576006637	40.0	39.0	41.0	35.0	41.0
31	38.91687658562447	40.0	39.0	41.0	35.0	41.0
32	38.84298760854133	40.0	38.0	41.0	35.0	41.0
33	38.768686780946446	40.0	38.0	41.0	35.0	41.0
34	38.747176203127665	40.0	38.0	41.0	35.0	41.0
35	38.63489527015896	40.0	38.0	41.0	35.0	41.0
36	38.59666206392519	40.0	38.0	41.0	35.0	41.0
37	38.53310080003834	40.0	38.0	41.0	35.0	41.0
38	38.45614016695671	40.0	38.0	41.0	35.0	41.0
39	38.38451412123082	40.0	38.0	41.0	34.0	41.0
40	38.29994698360685	40.0	38.0	41.0	34.0	41.0
41	38.217338756780556	40.0	38.0	41.0	34.0	41.0
42	38.1164219026595	40.0	37.0	41.0	34.0	41.0
43	37.21991020161206	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	3.0
16	2.0
17	6.0
18	11.0
19	31.0
20	49.0
21	112.0
22	199.0
23	320.0
24	529.0
25	756.0
26	1156.0
27	1653.0
28	2350.0
29	3269.0
30	4434.0
31	6024.0
32	7553.0
33	10487.0
34	15756.0
35	22316.0
36	35272.0
37	67703.0
38	168393.0
39	319333.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.68017037132442	19.852692304236218	12.65159842927703	25.815538895162327
2	19.447431400681126	19.86033025918127	34.380531901191816	26.31170643894578
3	19.400255796608747	23.027685340218476	28.890639461569105	28.68141940160367
4	14.381520342420004	16.52643780757745	35.59826154154898	33.493780308453566
5	14.89670789165486	36.01760024441456	34.055993697938355	15.029698165992231
6	32.702278506794784	36.218583294145134	15.959282211951752	15.11985598710833
7	29.678097640021683	29.622535261891997	21.778954588613754	18.920412509472563
8	27.30269365211062	32.42311874174427	19.713112421710964	20.561075184434145
9	26.715170176631453	14.488152184005823	20.55463534006871	38.24204229929401
10	19.040073803611705	25.212439982148155	31.775240445816948	23.972245768423196
11	36.15433461431323	22.17058698432572	21.01036665179013	20.664711749570927
12	22.43866422651479	26.428372456635884	27.04719657100752	24.08576674584181
13	30.19822739539746	19.250342210334303	24.684372744182426	25.86705765008582
14	23.125331352457177	20.88741055355704	24.401169355925706	31.58608873806008
15	26.520027915976506	27.01769309798448	21.389418886416124	25.072860099622897
16	25.951075154481384	25.148341066138702	23.822032654503847	25.07855112487607
17	24.19329717036234	26.14172450046277	24.449543070577697	25.21543525859719
18	24.165291335563815	24.5774413749517	25.820481101303244	25.436786188181237
19	24.91860336249734	25.044105445712113	25.922620028215505	24.11467116357504
20	25.002021811603104	24.7012960561195	25.550157401777398	24.746524730500003
21	25.971592798157307	25.05833300884505	24.839078772775334	24.13099542022231
22	26.607190460643565	24.065249102165883	24.652173522355245	24.675386914835304
23	24.92743942802201	24.47889677977829	25.546712833861	25.046950958338698
24	24.190152130090848	25.334347733624075	24.51738608214845	25.958114054136626
25	25.342434980036483	24.19569339152157	25.458352178614323	25.00351944982762
26	24.42632967809764	25.553152678226436	25.3701412871901	24.650376356485822
27	24.84027688335495	24.78306710317829	24.803285219209307	25.573370794257457
28	24.317151851530134	24.656067381738996	26.4897756238412	24.53700514288966
29	24.5060040316421	24.503308282837967	26.33716628876262	24.653521396757313
30	24.057611147220832	25.517808416127767	25.788281879476067	24.636298557175333
31	25.239247706367056	24.910066824617576	25.192970685229394	24.65771478378597
32	23.937800089259238	24.539251600226443	25.624440257713587	25.898508052800732
33	23.18943026846663	24.49552056407046	26.393177958359665	25.92187120910324
34	25.160771463402217	23.209348856852742	26.343606133128056	25.286273546616982
35	24.605596973572677	24.17921937105185	26.698995683806636	24.516187971568836
36	23.944539461269578	24.34590650544092	25.858820639850954	25.850733393438546
37	24.233284110957023	22.53810740462291	27.19666086581461	26.03194761860546
38	23.227470279369435	22.84482371300459	28.00643385381254	25.921272153813433
39	22.925246885661313	22.64174396975969	28.738179890312978	25.694829254266022
40	22.928691453577706	22.53496236435142	29.658778106925375	24.877568075145497
41	21.90325856124891	22.728007931492037	29.92415960031031	25.44457390694874
42	21.796177428195733	23.213392480058946	29.775144596970577	25.21528549477474
43	21.012912636771812	22.419194929596024	30.185647234311492	26.38224519932067
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	31.0
1	33.5
2	36.0
3	118.5
4	201.0
5	201.0
6	236.0
7	271.0
8	247.0
9	223.0
10	390.0
11	557.0
12	557.0
13	954.0
14	1351.0
15	1957.0
16	2563.0
17	2442.5
18	2322.0
19	2322.0
20	2673.0
21	3024.0
22	2742.5
23	2461.0
24	2852.0
25	3243.0
26	3243.0
27	4160.0
28	5077.0
29	7540.5
30	10004.0
31	12602.5
32	15201.0
33	15201.0
34	18992.0
35	22783.0
36	26979.0
37	31175.0
38	34528.5
39	37882.0
40	37882.0
41	41660.0
42	45438.0
43	48657.0
44	51876.0
45	53002.0
46	54128.0
47	54128.0
48	55118.0
49	56108.0
50	55251.0
51	54394.0
52	53024.0
53	51654.0
54	51654.0
55	48231.0
56	44808.0
57	40630.0
58	36452.0
59	34769.0
60	33086.0
61	33086.0
62	30444.0
63	27802.0
64	24677.0
65	21552.0
66	19156.5
67	16761.0
68	16761.0
69	14221.5
70	11682.0
71	10108.5
72	8535.0
73	6647.5
74	4760.0
75	4760.0
76	3848.5
77	2937.0
78	2559.0
79	2181.0
80	2002.5
81	1824.0
82	1824.0
83	1568.0
84	1312.0
85	1113.5
86	915.0
87	767.5
88	620.0
89	620.0
90	484.0
91	348.0
92	222.5
93	97.0
94	62.5
95	28.0
96	28.0
97	19.0
98	10.0
99	7.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	667718.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.473212469497085
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.0358291070522	39.57798749443824
2	4.9780570303554015	4.527364900532498
3	1.8501323689702487	2.523943869326343
4	1.0899210570051407	1.9824884720069442
5	0.7082603818274513	1.6103437413283412
6	0.5319405012824168	1.4513426061567676
7	0.4138637214886054	1.3173799058467683
8	0.3330408090704237	1.211554837749807
9	0.2844355508041612	1.1640778412036452
>10	2.305429235689361	21.708214334282783
>50	0.2990532969692	9.447183666571027
>100	0.16772126132109752	12.612691868111714
>500	0.0016540558315690085	0.4997300031709735
>1k	6.616223326276034E-4	0.36569645927412303
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1219	0.1825620995689797	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1212	0.18151375281181575	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	874	0.13089358082304206	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	738	0.11052570096957098	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.497638224519932E-4
2	0.0	0.0	0.0	0.0	1.497638224519932E-4
3	0.0	0.0	0.0	0.0	1.497638224519932E-4
4	0.0	0.0	0.0	0.0	1.497638224519932E-4
5	0.0	0.0	0.0	0.0	1.497638224519932E-4
6	0.0	0.0	0.0	0.0	1.497638224519932E-4
7	0.0	0.0	0.0	0.0	1.497638224519932E-4
8	0.0	0.0	0.0	0.0	1.497638224519932E-4
9	0.0	0.0	0.0	0.0	1.497638224519932E-4
10	0.0	0.0	0.0	0.0	1.497638224519932E-4
11	0.0	0.0	0.0	0.0	1.497638224519932E-4
12	0.0	0.0	0.0	0.0	1.497638224519932E-4
13	0.0	0.0	0.0	0.0	1.497638224519932E-4
14	0.0	0.0	0.0	1.497638224519932E-4	1.497638224519932E-4
15	0.0	0.0	0.0	4.4929146735597965E-4	1.497638224519932E-4
16	0.0	0.0	0.0	7.48819112259966E-4	1.497638224519932E-4
17	0.0	0.0	0.0	0.002096693514327905	1.497638224519932E-4
18	0.0	0.0	0.0	0.0026957488041358777	1.497638224519932E-4
19	0.0	1.497638224519932E-4	0.0	0.0038938593837518232	1.497638224519932E-4
20	0.0	1.497638224519932E-4	0.0	0.004043623206203817	1.497638224519932E-4
21	0.0	1.497638224519932E-4	0.0	0.005091969963367769	2.995276449039864E-4
22	0.0	1.497638224519932E-4	0.0	0.005840789075627735	2.995276449039864E-4
23	0.0	1.497638224519932E-4	0.0	0.00793748258995564	2.995276449039864E-4
24	0.0	1.497638224519932E-4	0.0	0.011981105796159457	2.995276449039864E-4
25	0.0	1.497638224519932E-4	0.0	0.016024729002363274	2.995276449039864E-4
26	0.0	1.497638224519932E-4	0.0	0.02471103070457888	2.995276449039864E-4
27	0.0	1.497638224519932E-4	0.0	0.05631119724194945	2.995276449039864E-4
28	0.0	1.497638224519932E-4	0.0	0.1807649336995558	2.995276449039864E-4
29	0.0	1.497638224519932E-4	0.0	0.33621978140472475	4.4929146735597965E-4
30	0.0	1.497638224519932E-4	0.0	0.5761414249728178	4.4929146735597965E-4
31	0.0	1.497638224519932E-4	0.0	1.2461847666230355	4.4929146735597965E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGGTTC	25	0.0054947147	29.6	20
GGTATCA	845	0.0	24.958578	1
GTATGCC	55	1.9011106E-5	23.545454	11
AGCGTCA	65	2.6800517E-6	22.76923	3
GCAGTCG	205	0.0	22.560976	9
TTACGGA	60	3.723277E-5	21.583332	37
ACCCGAT	60	3.723277E-5	21.583332	6
ATTAGAC	70	5.096639E-6	21.142859	3
TCGGTGA	220	0.0	21.022728	13
TAAGTTA	45	0.0038241548	20.555555	5
TGTCGTA	45	0.0038241548	20.555555	30
GGACTAT	55	5.1399914E-4	20.181818	6
AAGATAC	55	5.1399914E-4	20.181818	26
GCTTACA	65	6.896595E-5	19.923077	2
CGGTGAT	235	0.0	19.680851	14
TCTAGCG	85	1.2437085E-6	19.588236	28
CTAGCGG	85	1.2437085E-6	19.588236	29
CGCGGTT	95	1.6734884E-7	19.473684	10
CAGTCGG	230	0.0	19.304348	10
GGCAGTC	235	0.0	18.893618	8
>>END_MODULE