FastQCFastQC Report
Fri 10 Feb 2017
ERR1631277.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631277.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7738
Sequences flagged as poor quality0
Sequence length43
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT270.34892737141380203No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT250.32308089945722407No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA200.25846471956577927No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT180.23261824760920136No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT160.20677177565262342No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA150.19384853967433446No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC140.18092530369604548No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT140.18092530369604548No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT140.18092530369604548TruSeq Adapter, Index 1 (95% over 21bp)
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA120.15507883173946757No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT120.15507883173946757No Hit
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC120.15507883173946757No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA110.1421555957611786No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA110.1421555957611786No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT110.1421555957611786No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTAT100.12923235978288963No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACCTGTCT90.11630912380460068No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGT90.11630912380460068No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC80.10338588782631171No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.10338588782631171No Hit
CTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTC80.10338588782631171RNA PCR Primer, Index 1 (95% over 22bp)
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT80.10338588782631171No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT80.10338588782631171No Hit
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC80.10338588782631171No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC400.001763394923.12500237
TCTTATA450.00349640820.55555736
CTCTTAT750.003633464214.837
Produced by FastQC (version 0.11.5)