##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631277.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7738
Sequences flagged as poor quality	0
Sequence length	43
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.65921426725252	34.0	31.0	34.0	31.0	34.0
2	32.823597828896354	34.0	31.0	34.0	31.0	34.0
3	32.85060739209098	34.0	31.0	34.0	31.0	34.0
4	36.32088394934092	37.0	37.0	37.0	35.0	37.0
5	36.26117859912122	37.0	37.0	37.0	35.0	37.0
6	36.32682863789093	37.0	37.0	37.0	35.0	37.0
7	36.322563970018095	37.0	37.0	37.0	35.0	37.0
8	36.285991212199534	37.0	37.0	37.0	35.0	37.0
9	38.12664771258723	39.0	39.0	39.0	37.0	39.0
10	38.068880847764284	39.0	38.0	39.0	37.0	39.0
11	38.15611269061773	39.0	39.0	39.0	37.0	39.0
12	37.91729128973895	39.0	38.0	39.0	35.0	39.0
13	38.123546135952445	39.0	39.0	39.0	37.0	39.0
14	39.51214784181959	41.0	39.0	41.0	37.0	41.0
15	39.52907728095115	41.0	39.0	41.0	37.0	41.0
16	39.43241147583355	40.0	39.0	41.0	37.0	41.0
17	39.35603515120186	40.0	39.0	41.0	37.0	41.0
18	39.42724218144223	41.0	39.0	41.0	37.0	41.0
19	39.39971568880848	41.0	39.0	41.0	37.0	41.0
20	39.40604807443784	41.0	39.0	41.0	37.0	41.0
21	39.35151201860946	41.0	39.0	41.0	36.0	41.0
22	39.31610235202895	41.0	39.0	41.0	36.0	41.0
23	39.29852675109848	41.0	39.0	41.0	36.0	41.0
24	39.23520289480486	41.0	39.0	41.0	36.0	41.0
25	39.26092013440165	41.0	39.0	41.0	36.0	41.0
26	39.124709227190486	40.0	39.0	41.0	36.0	41.0
27	39.102222796588265	40.0	39.0	41.0	35.0	41.0
28	38.97983975187387	40.0	39.0	41.0	35.0	41.0
29	38.89028172654432	40.0	39.0	41.0	35.0	41.0
30	38.772680279141895	40.0	38.0	41.0	35.0	41.0
31	38.65003876970793	40.0	38.0	41.0	35.0	41.0
32	38.54135435513052	40.0	38.0	41.0	35.0	41.0
33	38.396484879813904	40.0	38.0	41.0	34.0	41.0
34	38.28767123287671	40.0	37.0	41.0	34.0	41.0
35	38.18260532437322	40.0	37.0	41.0	34.0	41.0
36	38.056603773584904	40.0	37.0	41.0	34.0	41.0
37	37.933057637632466	40.0	37.0	41.0	33.0	41.0
38	37.77009563194624	40.0	36.0	41.0	33.0	41.0
39	37.638795554406826	40.0	36.0	41.0	33.0	41.0
40	37.52106487464461	40.0	35.0	41.0	33.0	41.0
41	37.37438614629103	39.0	35.0	41.0	33.0	41.0
42	37.23235978288964	39.0	35.0	41.0	33.0	41.0
43	36.12923235978289	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	1.0
20	2.0
21	2.0
22	2.0
23	3.0
24	9.0
25	17.0
26	9.0
27	28.0
28	38.0
29	38.0
30	55.0
31	87.0
32	108.0
33	149.0
34	213.0
35	297.0
36	521.0
37	985.0
38	1857.0
39	3316.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.27009563194624	15.068493150684931	12.470922719048849	27.190488498319983
2	22.693202377875423	18.45438097699664	30.317911605065905	28.53450504006203
3	21.762729387438615	19.863013698630137	27.47479968984234	30.899457224088913
4	16.024812613078314	15.83096407340398	28.883432411475834	39.26079090204187
5	18.247609201344016	32.94132850865857	29.93021452571724	18.880847764280176
6	34.91858361333678	30.162832773326443	16.61928146807961	18.299302145257172
7	32.993021452571725	28.353579736365987	17.045748255363144	21.60765055569915
8	33.768415611269056	28.108038252778496	16.309123804600674	21.81442233135177
9	30.53760661669682	12.432153011113984	15.482036701990179	41.548203670199015
10	21.56888084776428	22.602739726027394	23.70121478418196	32.127164642026365
11	39.42879296975963	20.67717756526234	15.882657017317136	24.011372447660897
12	24.838459550271388	24.528301886792452	22.602739726027394	28.030498836908762
13	36.249676919100544	15.934349961230293	21.491341431894543	26.32463168777462
14	26.10493667614371	17.278366502972347	20.57379167743603	36.04290514344792
15	32.79917291289739	21.56888084776428	17.536831222538122	28.09511501680021
16	30.382527784957354	21.013181700697857	20.599638149392607	28.004652364952182
17	26.221245799948306	24.851382786249676	20.883949340914963	28.043422072887054
18	28.21142414060481	20.005169294391315	22.82243473765831	28.960971827345567
19	28.896355647454126	23.81752390798656	21.362109072111657	25.92401137244766
20	29.99483070560868	19.126389247867667	23.223055052985266	27.65572499353838
21	32.7862496769191	18.131300077539418	21.426725252003102	27.65572499353838
22	31.829930214525714	19.59162574308607	19.46239338330318	29.116050659085037
23	28.728353579736364	20.470405789609718	20.276557249935383	30.524683380718532
24	29.05143447919359	20.483329025588006	22.602739726027394	27.862496769191004
25	27.84957353321272	20.10855518221763	20.92271904884983	31.119152235719827
26	29.232359782889635	19.630395451020934	21.594727319720857	29.54251744636857
27	28.81881623158439	20.392866373739984	20.121478418195913	30.66683897647971
28	27.78495735332127	20.09563194623934	22.8353579736366	29.28405272680279
29	27.78495735332127	21.18118376841561	23.67536831222538	27.358490566037734
30	28.17265443266994	20.67717756526234	23.972602739726025	27.17756526234169
31	27.035409666580513	22.124579994830707	21.840268803308348	28.99974153528043
32	26.983716722667356	20.948565520806408	24.218144223313516	27.84957353321272
33	26.996639958645645	19.0876195399328	24.269837167226672	29.645903334194884
34	27.823727061256136	20.250710777978806	23.248901524941846	28.676660635823207
35	26.014474024295687	19.966399586456447	24.269837167226672	29.749289222021197
36	25.78185577668648	19.268544843628845	26.066166968208837	28.883432411475834
37	24.980615146032566	19.07469630395451	26.505556991470662	29.439131558542257
38	24.32153011113983	17.08451796329801	28.444042388214008	30.14990953734815
39	23.804600672008274	17.73067976221246	29.503747738433706	28.960971827345567
40	22.80951150168002	17.278366502972347	29.82682863789093	30.085293357456706
41	20.41871284569656	18.764538640475575	31.532695787025073	29.28405272680279
42	20.392866373739984	19.397777203411735	31.080382527784955	29.128973895063325
43	18.777461876453863	19.17808219178082	32.24347376583096	29.800982165934347
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	1.0
4	1.0
5	1.0
6	2.0
7	3.0
8	2.5
9	2.0
10	7.0
11	12.0
12	12.0
13	17.5
14	23.0
15	35.5
16	48.0
17	45.5
18	43.0
19	43.0
20	51.5
21	60.0
22	43.0
23	26.0
24	26.0
25	26.0
26	26.0
27	20.0
28	14.0
29	20.5
30	27.0
31	37.5
32	48.0
33	48.0
34	47.5
35	47.0
36	60.5
37	74.0
38	93.5
39	113.0
40	113.0
41	128.5
42	144.0
43	209.5
44	275.0
45	325.5
46	376.0
47	376.0
48	529.0
49	682.0
50	685.0
51	688.0
52	623.0
53	558.0
54	558.0
55	551.0
56	544.0
57	567.0
58	590.0
59	607.5
60	625.0
61	625.0
62	612.5
63	600.0
64	573.5
65	547.0
66	522.5
67	498.0
68	498.0
69	446.5
70	395.0
71	331.0
72	267.0
73	214.5
74	162.0
75	162.0
76	135.0
77	108.0
78	83.5
79	59.0
80	45.0
81	31.0
82	31.0
83	23.0
84	15.0
85	9.0
86	3.0
87	2.5
88	2.0
89	2.0
90	1.0
91	0.0
92	0.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	7738.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	91.71620573791678
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.3505706636607	88.36908761953993
2	2.395378328871354	4.393900232618248
3	0.47907566577427085	1.3181700697854741
4	0.22544737212906862	0.8270871026104937
5	0.09863322530646752	0.4523132592401137
6	0.07045230379033394	0.3876970793486689
7	0.04227138227420037	0.27138795554406825
8	0.08454276454840073	0.6203153269578703
9	0.028180921516133578	0.23261824760920136
>10	0.22544737212906862	3.1274231067459293
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	27	0.34892737141380203	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	25	0.32308089945722407	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	20	0.25846471956577927	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	18	0.23261824760920136	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT	16	0.20677177565262342	No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA	15	0.19384853967433446	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC	14	0.18092530369604548	No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT	14	0.18092530369604548	No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT	14	0.18092530369604548	TruSeq Adapter, Index 1 (95% over 21bp)
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA	12	0.15507883173946757	No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT	12	0.15507883173946757	No Hit
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC	12	0.15507883173946757	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA	11	0.1421555957611786	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	11	0.1421555957611786	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT	11	0.1421555957611786	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTAT	10	0.12923235978288963	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACCTGTCT	9	0.11630912380460068	No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGT	9	0.11630912380460068	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC	8	0.10338588782631171	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8	0.10338588782631171	No Hit
CTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTC	8	0.10338588782631171	RNA PCR Primer, Index 1 (95% over 22bp)
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT	8	0.10338588782631171	No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT	8	0.10338588782631171	No Hit
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC	8	0.10338588782631171	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.012923235978288964	0.0
23	0.0	0.0	0.0	0.03876970793486689	0.0
24	0.0	0.0	0.0	0.03876970793486689	0.0
25	0.0	0.0	0.0	0.06461617989144482	0.0
26	0.0	0.0	0.0	0.09046265184802274	0.0
27	0.0	0.0	0.0	0.19384853967433446	0.0
28	0.0	0.0	0.0	0.6720082708710261	0.0
29	0.0	0.0	0.0	1.3957094856552081	0.0
30	0.0	0.0	0.0	2.093564228482812	0.0
31	0.0	0.0	0.0	3.7477384337037996	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	40	0.0017633949	23.125002	37
TCTTATA	45	0.003496408	20.555557	36
CTCTTAT	75	0.0036334642	14.8	37
>>END_MODULE