FastQCFastQC Report
Fri 10 Feb 2017
ERR1631274.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631274.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136995
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8820.6438191174860396No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7250.5292163947589328No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT6590.4810394539946713No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA5070.3700864995072813No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC4030.2941713201211723No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC3710.27081280338698493No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC3680.2686229424431549No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG3190.23285521369393042No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2860.20876674331179967No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC2860.20876674331179967No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT2840.207306836015913No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC2690.19635753129676267No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC2280.16642943173108507No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCT2260.16496952443519836No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT2240.16350961713931164No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT2210.16131975619548158No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC2190.15985984889959487No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC2170.15839994160370818No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG2070.1511004051242746No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG2050.1496404978283879No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC2040.14891054418044453No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG2010.14672068323661447No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG1970.14380086864484104No Hit
GAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGG1890.1379612394612942No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC1860.13577137851746415No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC1830.13358151757363407No Hit
GACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTC1800.131391656629804No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG1790.13066170298186064No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA1780.1299317493339173No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCAATTATTC1780.1299317493339173No Hit
ATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGG1780.1299317493339173No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCT1770.12920179568597392No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC1770.12920179568597392No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC1770.12920179568597392No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA1750.1277418883900872No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGG1700.12409212015037044No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA1690.1233621665024271No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAAT1650.12044235191065367No Hit
GCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGC1630.11898244461476697No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAG1610.11752253731888025No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC1610.11752253731888025No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAG1590.11606263002299354No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGG1580.11533267637505018No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAA1570.11460272272710682No Hit
GCGTGTGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGA1570.11460272272710682No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCG1550.11314281543122011No Hit
GTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACAC1530.1116829081353334No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT1510.11022300083944671No Hit
TCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGC1510.11022300083944671No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG1490.10876309354356No Hit
TATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCC1460.10657323259972992No Hit
GTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGA1450.10584327895178655No Hit
GACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTC1420.1036534180079565No Hit
TAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGC1410.10292346436001315No Hit
GGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCGCGGGTAACC1370.10000364976823972No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAAGC200.001836281437.03
ACCTCTA352.373091E-531.71428525
CTTGAAG250.00547966329.62
AGAATTT405.9025857E-527.7517
TACACAT405.9025857E-527.7537
TTCACCT451.3149163E-424.66666622
GGTATCA3900.022.7692321
CACCTCT502.6852987E-422.224
CGGTCCA502.6852987E-422.210
AATTTCA603.6955294E-521.58333419
ATTTCAC603.6955294E-521.58333420
TCTAGAT450.00380888220.5555552
TAGATAA450.00380888220.5555554
TAGCGGC555.1113707E-420.18181830
AAGAATT555.1113707E-420.18181816
TCTAGCG555.1113707E-420.18181828
GAATTTC555.1113707E-420.18181818
CTAGCGG555.1113707E-420.18181829
TTTCACC656.8455665E-519.92307521
TCGCCTT1205.0567905E-918.524
Produced by FastQC (version 0.11.5)