##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631274.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	136995
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.00421913208511	34.0	33.0	34.0	31.0	34.0
2	33.13512901930727	34.0	33.0	34.0	31.0	34.0
3	33.20172999014562	34.0	33.0	34.0	31.0	34.0
4	36.45679769334647	37.0	37.0	37.0	35.0	37.0
5	36.446892222343884	37.0	37.0	37.0	35.0	37.0
6	36.51778532063214	37.0	37.0	37.0	35.0	37.0
7	36.520522646811926	37.0	37.0	37.0	35.0	37.0
8	36.52895361144567	37.0	37.0	37.0	35.0	37.0
9	38.354538486806085	39.0	39.0	39.0	37.0	39.0
10	38.32605569546334	39.0	39.0	39.0	37.0	39.0
11	38.37938610898208	39.0	39.0	39.0	37.0	39.0
12	38.33271287273258	39.0	39.0	39.0	37.0	39.0
13	38.36002043870214	39.0	39.0	39.0	37.0	39.0
14	39.84732289499617	41.0	40.0	41.0	38.0	41.0
15	39.83330778495566	41.0	40.0	41.0	38.0	41.0
16	39.781502974561114	41.0	40.0	41.0	38.0	41.0
17	39.79364940326289	41.0	40.0	41.0	38.0	41.0
18	39.79143764370963	41.0	40.0	41.0	38.0	41.0
19	39.80466440381036	41.0	40.0	41.0	38.0	41.0
20	39.776729077703564	41.0	40.0	41.0	38.0	41.0
21	39.74813679331363	41.0	40.0	41.0	37.0	41.0
22	39.65067338224023	41.0	40.0	41.0	37.0	41.0
23	39.53773495383043	41.0	40.0	41.0	37.0	41.0
24	39.47541881090551	41.0	39.0	41.0	36.0	41.0
25	39.47420708784992	41.0	39.0	41.0	36.0	41.0
26	39.3677725464433	41.0	39.0	41.0	36.0	41.0
27	39.28102485492171	41.0	39.0	41.0	35.0	41.0
28	39.11120843826417	41.0	39.0	41.0	35.0	41.0
29	39.030468265265156	41.0	39.0	41.0	35.0	41.0
30	38.92128909814227	41.0	38.0	41.0	35.0	41.0
31	38.82870177743713	40.0	38.0	41.0	35.0	41.0
32	38.71393846490748	40.0	38.0	41.0	35.0	41.0
33	38.56226869593781	40.0	38.0	41.0	35.0	41.0
34	38.47492974196138	40.0	38.0	41.0	35.0	41.0
35	38.310106208255775	40.0	37.0	41.0	35.0	41.0
36	38.20932150808424	40.0	37.0	41.0	35.0	41.0
37	38.089207635315155	40.0	37.0	41.0	34.0	41.0
38	37.96997700646009	40.0	36.0	41.0	34.0	41.0
39	37.82127084930107	40.0	36.0	41.0	34.0	41.0
40	37.645549107631666	40.0	35.0	41.0	34.0	41.0
41	37.48544107449177	40.0	35.0	41.0	33.0	41.0
42	37.26468849228074	40.0	35.0	41.0	33.0	41.0
43	36.39902186211176	38.0	35.0	41.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	2.0
14	0.0
15	1.0
16	3.0
17	2.0
18	4.0
19	8.0
20	13.0
21	25.0
22	40.0
23	73.0
24	113.0
25	189.0
26	268.0
27	380.0
28	499.0
29	738.0
30	961.0
31	1293.0
32	1563.0
33	1986.0
34	3079.0
35	4528.0
36	7731.0
37	15477.0
38	28386.0
39	69632.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.03522026351327	17.6378699952553	12.521624876820322	24.80528486441111
2	21.585459323332966	19.704368772582942	30.160224825723564	28.54994707836052
3	22.12781488375488	21.03726413372751	27.968903974597616	28.866017007919996
4	14.893244278988286	15.424650534691049	31.575604949085733	38.10650023723493
5	16.960473009963867	35.11150041972335	29.60035037775101	18.327676192561775
6	34.53921675973576	32.24716230519362	15.898390452206284	17.315230482864337
7	30.516442205919926	29.73101208073287	18.358334245775392	21.394211467571807
8	31.981459177342238	27.146246213365448	18.352494616591848	22.519799992700463
9	28.25139603635169	12.69754370597467	19.230628855067703	39.820431402605934
10	22.16869228803971	23.431512098981713	26.744771706996605	27.655023905981967
11	39.26639658381693	20.81535822475273	17.62619073688821	22.292054454542136
12	24.868060878134237	25.588525128654332	23.641008795941456	25.902405197269974
13	32.67929486477609	16.757545895835616	24.225701667944087	26.33745757144421
14	25.639621884010367	20.009489397423263	21.816124676083067	32.5347640424833
15	29.844884849812036	24.91404795795467	19.578816745136685	25.662250447096607
16	28.1404430818643	23.546844775356764	22.051169750720828	26.261542392058107
17	26.644038103580424	24.140297091134713	21.49713493193182	27.71852987335304
18	27.152815796196943	20.096353881528522	24.91185809701084	27.838972225263696
19	26.2126354976459	23.864374612212124	23.473849410562426	26.449140479579548
20	27.674732654476443	20.518997043687726	24.158545932333297	27.647724369502534
21	30.62520529946348	21.832183656337822	23.074564765137414	24.46804627906128
22	30.34344319135735	20.72192415781598	22.794262564327163	26.14037008649951
23	27.36742216869229	20.70659513120917	23.885543268002483	28.040439432096058
24	25.666630168984266	22.83295010766816	24.130077740063506	27.370341983284064
25	26.611190189422974	21.6533450125917	23.48041899339392	28.25504580459141
26	26.640388335340702	23.83736632723822	23.60232125260046	25.91992408482061
27	28.60542355560422	20.788349939778826	23.92642067228731	26.679805832329645
28	25.73159604365123	22.1971604803095	24.94178619657652	27.129457279462756
29	24.554180809518595	22.50812073433337	25.976860469360197	26.96083798678784
30	23.816197671447863	24.32935508595204	26.09949268221468	25.754954560385418
31	25.95861162816161	23.630789444870253	23.04828643381145	27.362312493156683
32	25.604584108909084	22.228548487171064	24.30234680097814	27.86452060294171
33	23.99649622248987	21.843862914704918	25.387057921821963	28.77258294098325
34	26.249863133691008	22.219789043395743	25.080477389685758	26.449870433227492
35	25.848388627322166	21.786926530165335	26.175407861600785	26.189276980911714
36	23.176758275849483	23.61254060367167	25.730866090003286	27.479835030475563
37	24.88776962662871	21.721230701850434	26.625059308733896	26.76594036278696
38	23.381145297273623	20.25110405489251	27.501003686265925	28.86674696156794
39	24.946895872112123	20.525566626519215	27.48129493777145	27.04624256359721
40	23.11690207671813	20.802219059089747	28.5090696740757	27.571809190116426
41	21.983284061462097	21.506624329355088	29.907660863535167	26.602430745647652
42	23.092083652688054	21.238001386911932	30.20110223000839	25.468812730391623
43	20.98032774918793	20.03065805321362	29.629548523668745	29.35946567392971
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	54.0
1	33.0
2	12.0
3	56.5
4	101.0
5	101.0
6	135.5
7	170.0
8	185.5
9	201.0
10	284.0
11	367.0
12	367.0
13	631.5
14	896.0
15	1316.5
16	1737.0
17	1535.0
18	1333.0
19	1333.0
20	1429.5
21	1526.0
22	1148.0
23	770.0
24	747.0
25	724.0
26	724.0
27	729.0
28	734.0
29	836.0
30	938.0
31	1145.0
32	1352.0
33	1352.0
34	1646.0
35	1940.0
36	2460.0
37	2980.0
38	3478.5
39	3977.0
40	3977.0
41	4465.5
42	4954.0
43	5305.5
44	5657.0
45	6624.0
46	7591.0
47	7591.0
48	8702.0
49	9813.0
50	9595.5
51	9378.0
52	10101.0
53	10824.0
54	10824.0
55	10428.0
56	10032.0
57	10010.5
58	9989.0
59	10031.5
60	10074.0
61	10074.0
62	9796.5
63	9519.0
64	9056.0
65	8593.0
66	7730.0
67	6867.0
68	6867.0
69	5906.5
70	4946.0
71	4150.5
72	3355.0
73	2510.5
74	1666.0
75	1666.0
76	1317.5
77	969.0
78	872.5
79	776.0
80	748.5
81	721.0
82	721.0
83	617.0
84	513.0
85	450.0
86	387.0
87	335.0
88	283.0
89	283.0
90	236.5
91	190.0
92	123.5
93	57.0
94	39.0
95	21.0
96	21.0
97	13.0
98	5.0
99	4.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	136995.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.52742800832147
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.74472304859046	45.212598999963504
2	5.242952259526845	5.403116902076718
3	1.8302875761439297	2.829300339428446
4	1.0610568069131605	2.1869411292382934
5	0.7068989941918119	1.821234351618672
6	0.47032157529395097	1.4540676667031645
7	0.35699107522311946	1.2876382349720792
8	0.29182603768239124	1.20296361181065
9	0.19832837512395526	0.919741596408628
>10	1.7608726448505454	18.08314172050075
>50	0.21107805638192378	7.3739917515237785
>100	0.11899702507437314	10.201102230008395
>500	0.005666525003541578	2.024161465746925
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	882	0.6438191174860396	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	725	0.5292163947589328	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	659	0.4810394539946713	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	507	0.3700864995072813	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	403	0.2941713201211723	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	371	0.27081280338698493	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	368	0.2686229424431549	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	319	0.23285521369393042	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	286	0.20876674331179967	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	286	0.20876674331179967	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	284	0.207306836015913	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	269	0.19635753129676267	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	228	0.16642943173108507	No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCT	226	0.16496952443519836	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	224	0.16350961713931164	No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT	221	0.16131975619548158	No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC	219	0.15985984889959487	No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC	217	0.15839994160370818	No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG	207	0.1511004051242746	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG	205	0.1496404978283879	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC	204	0.14891054418044453	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG	201	0.14672068323661447	No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG	197	0.14380086864484104	No Hit
GAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGG	189	0.1379612394612942	No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC	186	0.13577137851746415	No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC	183	0.13358151757363407	No Hit
GACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTC	180	0.131391656629804	No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG	179	0.13066170298186064	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA	178	0.1299317493339173	No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCAATTATTC	178	0.1299317493339173	No Hit
ATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGG	178	0.1299317493339173	No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCT	177	0.12920179568597392	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC	177	0.12920179568597392	No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC	177	0.12920179568597392	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA	175	0.1277418883900872	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGG	170	0.12409212015037044	No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA	169	0.1233621665024271	No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAAT	165	0.12044235191065367	No Hit
GCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGC	163	0.11898244461476697	No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAG	161	0.11752253731888025	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC	161	0.11752253731888025	No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAG	159	0.11606263002299354	No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGG	158	0.11533267637505018	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAA	157	0.11460272272710682	No Hit
GCGTGTGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGA	157	0.11460272272710682	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCG	155	0.11314281543122011	No Hit
GTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACAC	153	0.1116829081353334	No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT	151	0.11022300083944671	No Hit
TCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGC	151	0.11022300083944671	No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG	149	0.10876309354356	No Hit
TATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCC	146	0.10657323259972992	No Hit
GTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGA	145	0.10584327895178655	No Hit
GACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTC	142	0.1036534180079565	No Hit
TAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGC	141	0.10292346436001315	No Hit
GGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCGCGGGTAACC	137	0.10000364976823972	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	7.299536479433556E-4	0.0
11	0.0	0.0	0.0	0.0014599072958867113	0.0
12	0.0	0.0	0.0	0.0021898609438300667	0.0
13	0.0	0.0	0.0	0.0021898609438300667	0.0
14	0.0	0.0	0.0	0.0021898609438300667	0.0
15	0.0	0.0	0.0	0.0021898609438300667	0.0
16	0.0	0.0	0.0	0.003649768239716778	0.0
17	0.0	0.0	0.0	0.005839629183546845	0.0
18	0.0	0.0	0.0	0.0065695828314902005	0.0
19	0.0	0.0	0.0	0.007299536479433556	0.0
20	0.0	0.0	0.0	0.008029490127376911	0.0
21	0.0	0.0	0.0	0.010219351071206978	0.0
22	0.0	0.0	0.0	0.01167925836709369	0.0
23	0.0	0.0	0.0	0.017518887550640534	0.0
24	0.0	0.0	0.0	0.032847914157451	0.0
25	0.0	0.0	0.0	0.04379721887660134	0.0
26	0.0	0.0	0.0	0.065695828314902	0.0
27	0.0	0.0	0.0	0.14745063688455784	0.0
28	0.0	0.0	0.0	0.39417496988941203	0.0
29	0.0	0.0	0.0	0.7117048067447717	0.0
30	0.0	0.0	0.0	1.1066097302821272	0.0
31	0.0	0.0	0.0	1.9117486039636482	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGAAGC	20	0.0018362814	37.0	3
ACCTCTA	35	2.373091E-5	31.714285	25
CTTGAAG	25	0.005479663	29.6	2
AGAATTT	40	5.9025857E-5	27.75	17
TACACAT	40	5.9025857E-5	27.75	37
TTCACCT	45	1.3149163E-4	24.666666	22
GGTATCA	390	0.0	22.769232	1
CACCTCT	50	2.6852987E-4	22.2	24
CGGTCCA	50	2.6852987E-4	22.2	10
AATTTCA	60	3.6955294E-5	21.583334	19
ATTTCAC	60	3.6955294E-5	21.583334	20
TCTAGAT	45	0.003808882	20.555555	2
TAGATAA	45	0.003808882	20.555555	4
TAGCGGC	55	5.1113707E-4	20.181818	30
AAGAATT	55	5.1113707E-4	20.181818	16
TCTAGCG	55	5.1113707E-4	20.181818	28
GAATTTC	55	5.1113707E-4	20.181818	18
CTAGCGG	55	5.1113707E-4	20.181818	29
TTTCACC	65	6.8455665E-5	19.923075	21
TCGCCTT	120	5.0567905E-9	18.5	24
>>END_MODULE