FastQCFastQC Report
Fri 10 Feb 2017
ERR1631273.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631273.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1062991
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA22650.21307800348262593No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC20180.18984168257304154No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC19660.18494982553944483No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC16730.15738609263860184No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC16130.15174164221522102No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC15830.1489194170035306No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT13980.13151569486477308No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC13590.12784680208957555No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG10720.10084751423107063No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATTG250.00549615929.68
TTACACG358.868957E-426.428575
GGTATCA10050.026.3233851
TGCATCA4850.022.88659914
GTTTAGG1001.2894816E-820.351
GTATTAT555.1427435E-420.1818181
GCATCAG5600.020.15178515
TATACAC2900.019.77586237
ACAGGCT6550.019.7709928
AGACCGT759.264697E-619.7333346
GTTCAAA2250.019.7333341
GCTGCAT5900.019.44067812
GACAGGC6000.019.4257
AGTCGGT4950.019.06060611
GCAGTCG5000.018.8699999
CTGCATC5900.018.81355913
ATCAGAA6000.018.80833417
CTTATAC9500.018.537
CATCGTA500.007034541618.524
ATTGCCG1103.8508006E-818.511
Produced by FastQC (version 0.11.5)