##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631273.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1062991
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.03786861789046	34.0	33.0	34.0	31.0	34.0
2	33.18888682970975	34.0	33.0	34.0	31.0	34.0
3	33.270119878719576	34.0	34.0	34.0	31.0	34.0
4	36.50581237282348	37.0	37.0	37.0	35.0	37.0
5	36.4983993279341	37.0	37.0	37.0	35.0	37.0
6	36.55538569940855	37.0	37.0	37.0	35.0	37.0
7	36.55602822601509	37.0	37.0	37.0	35.0	37.0
8	36.54629154903475	37.0	37.0	37.0	35.0	37.0
9	38.40776544674414	39.0	39.0	39.0	37.0	39.0
10	38.36233514676982	39.0	39.0	39.0	37.0	39.0
11	38.425348850554705	39.0	39.0	39.0	37.0	39.0
12	38.37849426759023	39.0	39.0	39.0	37.0	39.0
13	38.3952667520233	39.0	39.0	39.0	37.0	39.0
14	39.939467972917924	41.0	40.0	41.0	38.0	41.0
15	39.94843700464068	41.0	40.0	41.0	38.0	41.0
16	39.92111033865762	41.0	40.0	41.0	38.0	41.0
17	39.89875549275582	41.0	40.0	41.0	38.0	41.0
18	39.8469046304249	41.0	40.0	41.0	38.0	41.0
19	39.87212309417483	41.0	40.0	41.0	38.0	41.0
20	39.85890567276675	41.0	40.0	41.0	38.0	41.0
21	39.83482550651887	41.0	40.0	41.0	38.0	41.0
22	39.78200850242382	41.0	40.0	41.0	38.0	41.0
23	39.72310866225584	41.0	40.0	41.0	37.0	41.0
24	39.69626365604224	41.0	40.0	41.0	37.0	41.0
25	39.67180437087426	41.0	40.0	41.0	37.0	41.0
26	39.58465217485379	41.0	40.0	41.0	37.0	41.0
27	39.527546329178705	41.0	40.0	41.0	37.0	41.0
28	39.44612889478839	41.0	39.0	41.0	37.0	41.0
29	39.40130443249284	41.0	39.0	41.0	36.0	41.0
30	39.33945536697865	41.0	39.0	41.0	36.0	41.0
31	39.30984175783238	41.0	39.0	41.0	36.0	41.0
32	39.24071981794766	41.0	39.0	41.0	35.0	41.0
33	39.16309263201664	41.0	39.0	41.0	35.0	41.0
34	39.10318713893156	41.0	39.0	41.0	35.0	41.0
35	38.99366034143281	41.0	39.0	41.0	35.0	41.0
36	38.94234476115037	40.0	39.0	41.0	35.0	41.0
37	38.87781646316855	40.0	39.0	41.0	35.0	41.0
38	38.8089061901747	40.0	38.0	41.0	35.0	41.0
39	38.72079067461531	40.0	38.0	41.0	35.0	41.0
40	38.63183789890977	40.0	38.0	41.0	35.0	41.0
41	38.53925762306548	40.0	38.0	41.0	35.0	41.0
42	38.42430274574291	40.0	37.0	41.0	35.0	41.0
43	37.583100891729096	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	2.0
13	3.0
14	2.0
15	3.0
16	3.0
17	7.0
18	16.0
19	33.0
20	75.0
21	108.0
22	234.0
23	333.0
24	579.0
25	855.0
26	1225.0
27	1735.0
28	2480.0
29	3387.0
30	4595.0
31	6306.0
32	8340.0
33	11625.0
34	18581.0
35	29467.0
36	46689.0
37	85821.0
38	217894.0
39	622592.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.104230421518146	16.143598581737756	11.669901250339842	28.082269746404247
2	20.944203666823142	18.11115992515459	32.20300077799342	28.74163563002885
3	21.316267023897662	19.480973968735388	27.39976161604379	31.802997391323164
4	15.498626046692776	14.28008327445858	31.272042754830476	38.949247924018174
5	17.33796429132514	34.022959742838836	31.488037057698513	17.15103890813751
6	38.207378990038485	31.390670287895194	14.619596967424936	15.782353754641385
7	32.83113403594198	27.506159506524515	18.50335515540583	21.15935130212768
8	30.294988386543253	30.300068391924295	17.933453810991814	21.471489410540634
9	28.28151884634959	13.190892491093528	17.00211949113398	41.5254691714229
10	19.750590550625546	23.91741792733899	29.418217087444766	26.9137744345907
11	39.88509780421471	19.61615855637536	18.2207563375419	22.27798730186803
12	25.239536364842223	22.87347682153471	25.27820085024238	26.608785963380686
13	33.05220834419106	16.619049455733865	22.59567578653065	27.73306641354442
14	25.042262822545062	18.157256270278864	21.613165116167494	35.18731579100858
15	28.924703972093834	24.07696772597322	19.25472558093154	27.7436027210014
16	28.740412665770453	22.687115883389417	20.377030473447093	28.195440977393034
17	27.489602451949263	23.333405456866522	21.501216849437107	27.675775241747107
18	27.354700086830462	21.034420799423515	23.302360979537927	28.3085181342081
19	28.014630415497404	22.506963840709844	21.87224539060067	27.606160353192077
20	28.20974025179893	21.282306247183655	22.34289848173691	28.165055019280498
21	30.319165449190066	21.487858316768442	21.348440391310934	26.84453584273056
22	30.02518365630565	21.337245564637893	21.122850522723148	27.51472025633331
23	28.20127357616386	21.543362079265016	21.592562872122155	28.66280147244897
24	27.785653876655587	22.095295256497938	22.040450013217423	28.078600853629055
25	27.974648891665122	21.300744785233366	21.91655432642421	28.808051996677296
26	28.611342899422475	22.135182706156495	22.060205589699255	27.19326880472177
27	29.126963445598314	20.987948157604343	21.784191963995934	28.10089643280141
28	27.495058754025198	21.303567010445054	22.576860951786045	28.624513283743703
29	26.697121612506596	21.6814629662904	23.55034050147179	28.07107491973121
30	26.13380546025319	23.04243403754124	23.26068612057863	27.56307438162694
31	27.90729178327944	22.368016286120955	21.714953372135795	28.009738558463802
32	26.727601644792852	21.7881430792923	22.92258354021812	28.561671735696727
33	26.96410411753251	21.50751981907655	22.846101236981312	28.682274826409632
34	27.945579971984714	21.610436965129526	22.91195315858742	27.532029904298344
35	27.46956465294626	21.37017152544095	23.73576069787985	27.42450312373294
36	26.36108866396799	22.469710467915533	23.08241556137352	28.086785306742957
37	27.085930172503815	20.75652569024573	24.046581767860687	28.110962369389767
38	26.053372041720014	20.518706179073952	24.97970349701926	28.448218282186772
39	26.1465054737058	20.342034880822133	25.397204680001995	28.114254965470074
40	25.36060982642374	19.93798630468179	26.406150193181315	28.29525367571315
41	24.03952620483146	20.453983147552517	27.727516037294762	27.778974610321256
42	24.838404088087294	20.275712588347407	27.416600893140203	27.46928243042509
43	23.170939358846876	19.380690899546657	28.160163162246903	29.28820657935956
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5.0
1	10.5
2	16.0
3	41.0
4	66.0
5	66.0
6	99.5
7	133.0
8	140.0
9	147.0
10	200.0
11	253.0
12	253.0
13	467.5
14	682.0
15	1197.0
16	1712.0
17	1606.0
18	1500.0
19	1500.0
20	1725.0
21	1950.0
22	1770.0
23	1590.0
24	1936.5
25	2283.0
26	2283.0
27	2916.0
28	3549.0
29	5056.5
30	6564.0
31	7977.5
32	9391.0
33	9391.0
34	12427.5
35	15464.0
36	19114.5
37	22765.0
38	27428.5
39	32092.0
40	32092.0
41	36594.0
42	41096.0
43	45042.0
44	48988.0
45	54571.5
46	60155.0
47	60155.0
48	66295.5
49	72436.0
50	77162.0
51	81888.0
52	88803.5
53	95719.0
54	95719.0
55	93200.5
56	90682.0
57	87519.5
58	84357.0
59	81057.0
60	77757.0
61	77757.0
62	76291.5
63	74826.0
64	70023.5
65	65221.0
66	58462.5
67	51704.0
68	51704.0
69	43865.5
70	36027.0
71	31591.0
72	27155.0
73	23505.5
74	19856.0
75	19856.0
76	17788.0
77	15720.0
78	12721.5
79	9723.0
80	7038.0
81	4353.0
82	4353.0
83	3328.0
84	2303.0
85	1859.5
86	1416.0
87	1116.5
88	817.0
89	817.0
90	634.0
91	451.0
92	285.5
93	120.0
94	76.5
95	33.0
96	33.0
97	23.0
98	13.0
99	13.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1062991.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.50701174055865
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.818266832158	49.300365798286656
2	6.024947909349405	6.688537086810236
3	1.7335714154040567	2.886761067237896
4	0.8028498937621799	1.7825519391585436
5	0.4705725180406175	1.3060037141832406
6	0.31539076924283405	1.050383947873549
7	0.21443062323556802	0.8331682185030389
8	0.16724189085136976	0.742647807920017
9	0.14223331955544866	0.7105451884616629
>10	1.0400293255592712	11.691467960022386
>50	0.1418739690151814	5.472917370218734
>100	0.11753538362625066	12.501038455760602
>500	0.009015014778390219	3.325087742241368
>1k	0.0020411354215223135	1.7085237033221592
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	2265	0.21307800348262593	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	2018	0.18984168257304154	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	1966	0.18494982553944483	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	1673	0.15738609263860184	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	1613	0.15174164221522102	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	1583	0.1489194170035306	No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT	1398	0.13151569486477308	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	1359	0.12784680208957555	No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG	1072	0.10084751423107063	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	9.407417372301364E-5	0.0
3	0.0	0.0	0.0	9.407417372301364E-5	0.0
4	0.0	0.0	0.0	9.407417372301364E-5	0.0
5	0.0	0.0	0.0	9.407417372301364E-5	0.0
6	0.0	0.0	0.0	9.407417372301364E-5	0.0
7	0.0	0.0	0.0	9.407417372301364E-5	0.0
8	0.0	0.0	0.0	9.407417372301364E-5	0.0
9	0.0	0.0	0.0	9.407417372301364E-5	0.0
10	0.0	0.0	0.0	9.407417372301364E-5	0.0
11	0.0	0.0	0.0	1.881483474460273E-4	0.0
12	0.0	0.0	0.0	1.881483474460273E-4	0.0
13	0.0	0.0	0.0	1.881483474460273E-4	0.0
14	0.0	0.0	0.0	1.881483474460273E-4	0.0
15	0.0	0.0	0.0	5.644450423380818E-4	0.0
16	0.0	0.0	0.0	0.00103481591095315	0.0
17	0.0	0.0	0.0	0.0022577801693523273	0.0
18	0.0	0.0	0.0	0.002351854343075341	0.0
19	0.0	0.0	0.0	0.002445928516798355	0.0
20	0.0	0.0	0.0	0.0030103735591364366	9.407417372301364E-5
21	0.0	0.0	0.0	0.003480744427751505	9.407417372301364E-5
22	0.0	0.0	0.0	0.005456302075934791	9.407417372301364E-5
23	0.0	0.0	0.0	0.008090378940179173	9.407417372301364E-5
24	0.0	0.0	0.0	0.011947420062822733	9.407417372301364E-5
25	0.0	0.0	0.0	0.014487422753344102	9.407417372301364E-5
26	0.0	0.0	0.0	0.019191131439494784	9.407417372301364E-5
27	0.0	0.0	0.0	0.03612448270963724	9.407417372301364E-5
28	0.0	0.0	0.0	0.12389568679320898	9.407417372301364E-5
29	0.0	0.0	0.0	0.2542824915733059	9.407417372301364E-5
30	0.0	0.0	0.0	0.4299189739141724	9.407417372301364E-5
31	0.0	0.0	0.0	0.9401772921877984	9.407417372301364E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATTG	25	0.005496159	29.6	8
TTACACG	35	8.868957E-4	26.42857	5
GGTATCA	1005	0.0	26.323385	1
TGCATCA	485	0.0	22.886599	14
GTTTAGG	100	1.2894816E-8	20.35	1
GTATTAT	55	5.1427435E-4	20.181818	1
GCATCAG	560	0.0	20.151785	15
TATACAC	290	0.0	19.775862	37
ACAGGCT	655	0.0	19.770992	8
AGACCGT	75	9.264697E-6	19.733334	6
GTTCAAA	225	0.0	19.733334	1
GCTGCAT	590	0.0	19.440678	12
GACAGGC	600	0.0	19.425	7
AGTCGGT	495	0.0	19.060606	11
GCAGTCG	500	0.0	18.869999	9
CTGCATC	590	0.0	18.813559	13
ATCAGAA	600	0.0	18.808334	17
CTTATAC	950	0.0	18.5	37
CATCGTA	50	0.0070345416	18.5	24
ATTGCCG	110	3.8508006E-8	18.5	11
>>END_MODULE