FastQCFastQC Report
Fri 10 Feb 2017
ERR1631272.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631272.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36415
Sequences flagged as poor quality0
Sequence length43
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA980.269119868186187No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT950.26088150487436496RNA PCR Primer, Index 26 (100% over 22bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT830.22792805162707677No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT620.1702595084443224No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA620.1702595084443224No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT560.1537827818206783No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC530.14554441850885624No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA500.1373060551970342No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGT480.13181381298915282No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT470.12906769188521214No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTG440.12082932857339007No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC430.1180832074694494No Hit
GTTCCGACCTGCACGAATGGCGTAACGATGGCCACACTGTCTC410.11259096526156803No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT410.11259096526156803No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA380.10435260194974598No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA380.10435260194974598No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT370.1016064808458053No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT370.1016064808458053No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAC250.0054275729.59999816
GCCTTAT555.98664E-726.9090926
CGCCTTA555.98664E-726.9090925
ATACACA358.704803E-426.4285737
TCCTCGC601.279006E-624.66666621
GGTATCA1500.024.6666661
TATACAC400.001895687723.12536
TCGCCTT652.5664085E-622.7692324
CCTTATT652.5664085E-622.7692327
CTCGCCT652.5664085E-622.7692323
GGTGATT753.539426E-722.215
GTGATTC753.539426E-722.216
GTATCAA1700.021.7647062
CAGTCGG704.8818565E-621.14285710
GCAGTCG704.8818565E-621.1428579
CTTATTG704.8818565E-621.14285728
GATTCCT704.8818565E-621.14285718
ATTCCTC704.8818565E-621.14285719
TGATTCC806.587543E-720.812517
GGCAGTC656.670531E-519.9230778
Produced by FastQC (version 0.11.5)