FastQCFastQC Report
Fri 10 Feb 2017
ERR1631271.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631271.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences571941
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT23130.40441234323120745No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT21420.3745141544320131No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT17150.2998561040387033No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA9310.16277902790672463No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC8980.1570092019981082No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8580.15001547362402765No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC6530.11417261570686486No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC6480.1132983996601048No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC6390.11172481077593667No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG6200.10840278979824841No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGCAT352.3862262E-531.71428512
CTCGAAT250.00549406329.612
GCAGTCG2450.023.4081639
ACTGATC909.4574716E-820.5555558
AGTCGGT2700.020.55555511
CGCCTTA2200.020.18181825
CGGAGTC555.1387533E-420.18181827
GGCAGTC2850.020.1228078
GTCTAGA656.89438E-519.9230771
TGGTATA656.89438E-519.9230777
CAGTCGG2900.019.77586210
TCGGTGA2900.019.77586213
GTATAAT759.25243E-619.7333341
GCCTTAT2300.019.30434826
GTCGGTG3000.019.11666712
TGAAGCG500.00703066618.55
ACGGAGT609.2290813E-418.526
CTACAGG500.00703066618.53
GGTATCA12050.018.269711
CGGTGAT3050.018.19672214
Produced by FastQC (version 0.11.5)