FastQCFastQC Report
Fri 10 Feb 2017
ERR1631270.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631270.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences888160
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA9420.10606197081606918No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6950.026.6187061
GATTTAG400.001930843623.1251
CCCTTAA656.899875E-519.9230771
GTTCTAG851.2446562E-619.5882341
CTTATAC13850.019.3682337
ATACACT1054.7942194E-717.6190474
TCTACTT2000.017.5749992
GTATCAA10550.017.5355452
TAAGGCA650.001579552217.0769234
TGTAAGG650.001579552217.0769232
TACCGTC1201.0403892E-716.9583327
ATACCGT1107.8026824E-716.8181826
CGCTATC1801.0913936E-1116.44444512
TATACAC3500.016.38571437
TTATACA6950.016.2374137
ACTAGCC803.381483E-416.18753
GATCTAG700.002591871215.8571431
ATTAGAG1059.338239E-615.8571423
CTAAGTG957.056787E-515.57894819
TAAGTGT957.056787E-515.57894820
Produced by FastQC (version 0.11.5)