FastQCFastQC Report
Fri 10 Feb 2017
ERR1631269.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631269.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1412172
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT31650.22412284056049828No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT27830.1970723113048552No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA26430.18715850477137344No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT23250.16464000135960774No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC21140.14969847865557454No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC20230.1432545044088114No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC19960.14134255600592563No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC16640.11783267194081175No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA15250.10798967831114056No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAAG551.903058E-523.5454541
GGTATCA24350.021.0451761
GCAGTCG13150.020.6806099
CAGTCGG13150.020.25855410
AGTCGGT13050.020.13026811
GTACTAT1254.129106E-1019.241
CTCTATG21550.018.8863121
GGGCAGT14700.018.7517017
CTTATAC17850.018.65546237
TAAGTCG500.007035659618.521
GTATATG701.2194477E-418.51
TCTATGG21850.018.4576662
GGCAGTC14650.018.310588
TCGGTGA14550.018.1821313
CCGCTTA4350.017.86206825
CGGTGAT15300.017.2908514
GTCGGTG15300.017.2908512
GTCTTAC752.0676876E-417.2666661
GTAAGGT752.0676876E-417.26666619
GCTTTAT3350.017.1194041
Produced by FastQC (version 0.11.5)