FastQCFastQC Report
Fri 10 Feb 2017
ERR1631268.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631268.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences804048
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT22380.27834159154677335No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT21260.26441207490100094No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT17610.2190167751179034No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA9060.11267984000955167No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9450.025.0581991
GGTATAC400.001930685523.1253
TAGAACA652.6811813E-622.769234
TCGTTTA1700.021.76470630
TTAGAAC705.0987783E-621.1428573
CGTTTAT1700.020.67647231
GCTAAAC450.003824822420.5555573
ATGGTCG1500.019.73333436
CAGTCGG2650.018.84905610
GTATTAC701.2186194E-418.51
TAGTACT500.007033089618.4999984
AGTCGGT2750.018.16363711
ATCAGTA752.066293E-417.2666662
GTATCAA14050.016.9857652
CCAATAC1005.873373E-616.653
CTCTATG4250.016.5411781
GTACTAT904.443282E-516.4444451
TGGTCGG1801.0913936E-1116.44444537
GCAGTCG3050.016.377059
TATGGTC2050.016.24390435
Produced by FastQC (version 0.11.5)