##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631268.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	804048
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.05114620022685	34.0	33.0	34.0	31.0	34.0
2	33.196484538236525	34.0	33.0	34.0	31.0	34.0
3	33.28493696893718	34.0	34.0	34.0	31.0	34.0
4	36.49223678188367	37.0	37.0	37.0	35.0	37.0
5	36.486296837999724	37.0	37.0	37.0	35.0	37.0
6	36.54227732672676	37.0	37.0	37.0	35.0	37.0
7	36.55378161502796	37.0	37.0	37.0	35.0	37.0
8	36.553030416094565	37.0	37.0	37.0	35.0	37.0
9	38.40225210435198	39.0	39.0	39.0	37.0	39.0
10	38.366420910194414	39.0	39.0	39.0	37.0	39.0
11	38.41819269496349	39.0	39.0	39.0	37.0	39.0
12	38.38157547808091	39.0	39.0	39.0	37.0	39.0
13	38.40875544743597	39.0	39.0	39.0	37.0	39.0
14	39.954955425546736	41.0	40.0	41.0	38.0	41.0
15	39.94626564583209	41.0	40.0	41.0	38.0	41.0
16	39.91107496069886	41.0	40.0	41.0	38.0	41.0
17	39.90590735876465	41.0	40.0	41.0	38.0	41.0
18	39.8796912622132	41.0	40.0	41.0	38.0	41.0
19	39.89742154697232	41.0	40.0	41.0	38.0	41.0
20	39.87151637713171	41.0	40.0	41.0	38.0	41.0
21	39.84176193461087	41.0	40.0	41.0	38.0	41.0
22	39.77854680317593	41.0	40.0	41.0	38.0	41.0
23	39.71910880942431	41.0	40.0	41.0	38.0	41.0
24	39.694350337293294	41.0	40.0	41.0	37.0	41.0
25	39.68907950769108	41.0	40.0	41.0	37.0	41.0
26	39.59890329930551	41.0	40.0	41.0	37.0	41.0
27	39.54462793266074	41.0	40.0	41.0	37.0	41.0
28	39.47507362744513	41.0	40.0	41.0	37.0	41.0
29	39.42302822717052	41.0	39.0	41.0	36.0	41.0
30	39.36279550474598	41.0	39.0	41.0	36.0	41.0
31	39.30259511869938	41.0	39.0	41.0	35.0	41.0
32	39.22982085646628	41.0	39.0	41.0	35.0	41.0
33	39.13866211967445	41.0	39.0	41.0	35.0	41.0
34	39.08932924402523	41.0	39.0	41.0	35.0	41.0
35	38.992730533500485	41.0	39.0	41.0	35.0	41.0
36	38.92956763775297	41.0	39.0	41.0	35.0	41.0
37	38.86554285316299	41.0	39.0	41.0	35.0	41.0
38	38.78943421288281	40.0	38.0	41.0	35.0	41.0
39	38.696087795753485	40.0	38.0	41.0	35.0	41.0
40	38.59598680675781	40.0	38.0	41.0	35.0	41.0
41	38.50612525620361	40.0	38.0	41.0	35.0	41.0
42	38.38057553777884	40.0	38.0	41.0	35.0	41.0
43	37.581547370306254	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	2.0
12	4.0
13	0.0
14	3.0
15	5.0
16	2.0
17	7.0
18	17.0
19	28.0
20	46.0
21	115.0
22	180.0
23	317.0
24	470.0
25	674.0
26	1081.0
27	1527.0
28	2181.0
29	3070.0
30	4164.0
31	5328.0
32	6869.0
33	9253.0
34	13464.0
35	20576.0
36	33394.0
37	65439.0
38	151431.0
39	484398.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.522401647662825	17.85204365908503	12.062215191132868	26.563339502119277
2	20.01659104929059	19.76038743955585	32.70376892921816	27.519252581935405
3	20.85696376335741	21.016406980677772	28.790196605177798	29.33643265078702
4	14.96751437725111	15.151085507332896	33.49103536107297	36.390364754343025
5	16.407105048454817	34.44645095815175	32.54071896205202	16.60572503134141
6	35.37313692714863	33.25622350904423	15.561633136330169	15.809006427476966
7	30.432387121166894	29.42610391419418	20.008755696177342	20.132753268461585
8	29.06816508467156	30.306897100670604	19.580298688635505	21.04463912602233
9	27.46353451535232	13.802907289117067	19.47271804668378	39.26084014884684
10	18.898871709151692	24.877121763874793	31.007477165542358	25.216529361431157
11	37.14467295484847	20.964420034624798	20.056140926909833	21.8347660836169
12	23.565135414801105	24.665940341870137	26.888817583029866	24.880106660298885
13	31.703704256462302	18.20612699739319	23.696968340198595	26.393200405945915
14	24.01921278331642	20.094695839054385	23.529316657711977	32.35677471991722
15	27.348242890971687	26.12120171930034	20.84875529819115	25.68180009153682
16	26.447550395001297	24.782848785147156	22.88383280600163	25.88576801384992
17	25.15521461405289	24.850630808110957	23.97966290569717	26.014491672138977
18	25.25719857520944	23.18331741388574	25.626703878375423	25.932780132529405
19	25.722096193262097	24.240343860068055	25.14439436451555	24.8931655821543
20	26.522297176287985	23.126231269774937	24.481374246313653	25.870097307623425
21	27.391275147752374	23.549464708574614	24.225916860684933	24.83334328298808
22	27.267153204783796	23.328831114560327	24.156642389509084	25.247373291146797
23	26.151050683541282	23.255949893538695	24.22803116231867	26.36496826060136
24	25.47459853143096	23.969464509581517	24.79628081905558	25.75965613993195
25	25.781918492428314	23.49374664199152	24.300663641971624	26.42367122360854
26	25.616007999522417	24.280266849740315	24.45836566971126	25.64535948102601
27	26.15254313175333	23.184809862097786	24.51632240861242	26.146324597536463
28	25.15670706226494	23.758158716892524	24.995771396732533	26.08936282411
29	24.5706723976678	24.30228046086801	25.52758044295863	25.599466698505562
30	24.766556225498977	24.56706564782202	25.17424332875649	25.49213479792251
31	25.4166417925298	24.618803852506318	24.344069010805324	25.620485344158556
32	24.800385051638706	23.846461902771974	24.879857918930213	26.473295126659107
33	24.322677253099318	23.75965116510457	25.569866475633297	26.347805106162813
34	25.780923526953615	22.956340914970248	25.355575786520205	25.907159771555925
35	25.142280062881817	23.146628062006247	25.918601874514952	25.792490000596977
36	24.07231906552843	24.04993234234772	25.865619962987285	26.012128629136573
37	24.841800489523013	22.679989254372874	26.285619763994188	26.192590492109925
38	24.11510258094044	22.565816966151274	26.96542992458162	26.353650528326668
39	24.217335283465662	22.22902115296599	27.371127096889737	26.182516466678607
40	23.395245059996416	22.30973772710087	28.106655324060252	26.188361888842458
41	22.564448888623563	22.407368714305615	28.875888006686168	26.152294390384657
42	23.038301195948502	22.50300977056096	28.841934809862096	25.61675422362844
43	22.128902752074502	21.91486080433009	29.030978250054723	26.925258193540685
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	144.0
1	135.5
2	127.0
3	253.0
4	379.0
5	379.0
6	494.5
7	610.0
8	554.5
9	499.0
10	764.5
11	1030.0
12	1030.0
13	1743.5
14	2457.0
15	3575.0
16	4693.0
17	4589.0
18	4485.0
19	4485.0
20	5457.5
21	6430.0
22	6100.5
23	5771.0
24	6786.0
25	7801.0
26	7801.0
27	8956.0
28	10111.0
29	11942.0
30	13773.0
31	15964.5
32	18156.0
33	18156.0
34	20711.5
35	23267.0
36	26576.5
37	29886.0
38	32150.0
39	34414.0
40	34414.0
41	36561.0
42	38708.0
43	40207.0
44	41706.0
45	43745.0
46	45784.0
47	45784.0
48	48996.0
49	52208.0
50	52948.5
51	53689.0
52	55473.0
53	57257.0
54	57257.0
55	56456.5
56	55656.0
57	55159.5
58	54663.0
59	53661.5
60	52660.0
61	52660.0
62	49751.5
63	46843.0
64	43870.0
65	40897.0
66	36328.0
67	31759.0
68	31759.0
69	28037.5
70	24316.0
71	20812.0
72	17308.0
73	13310.0
74	9312.0
75	9312.0
76	7449.0
77	5586.0
78	4744.0
79	3902.0
80	3366.0
81	2830.0
82	2830.0
83	2397.0
84	1964.0
85	1681.0
86	1398.0
87	1182.0
88	966.0
89	966.0
90	704.0
91	442.0
92	274.5
93	107.0
94	73.0
95	39.0
96	39.0
97	25.5
98	12.0
99	7.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	804048.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.00145681682926
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.0697366455852	61.6880648399602
2	4.463358381808687	5.981030277535754
3	1.0888839509461825	2.1887043305357725
4	0.5391603726686577	1.444981217068186
5	0.32647535720346915	1.0937162273713572
6	0.21992327511273463	0.8841107892288931
7	0.16197366785036493	0.759673019835777
8	0.12458285251141694	0.6677786090128889
9	0.10334863689954234	0.6232058309072568
>10	0.7311203063312398	9.72031045424951
>50	0.10051747184149816	4.723952650998366
>100	0.06868540939304206	8.683106010323497
>500	0.001675253886001841	0.7774798965685646
>1k	5.584179620006137E-4	0.7638858464039875
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2238	0.27834159154677335	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2126	0.26441207490100094	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1761	0.2190167751179034	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	906	0.11267984000955167	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.243706843372535E-4	0.0	0.0	0.0
3	0.0	1.243706843372535E-4	0.0	0.0	0.0
4	0.0	1.243706843372535E-4	0.0	0.0	0.0
5	0.0	1.243706843372535E-4	0.0	0.0	0.0
6	0.0	1.243706843372535E-4	0.0	0.0	0.0
7	0.0	1.243706843372535E-4	0.0	0.0	0.0
8	0.0	1.243706843372535E-4	0.0	0.0	0.0
9	0.0	2.48741368674507E-4	0.0	1.243706843372535E-4	0.0
10	0.0	2.48741368674507E-4	0.0	1.243706843372535E-4	0.0
11	0.0	2.48741368674507E-4	0.0	1.243706843372535E-4	0.0
12	0.0	2.48741368674507E-4	0.0	1.243706843372535E-4	0.0
13	0.0	2.48741368674507E-4	0.0	1.243706843372535E-4	0.0
14	0.0	3.731120530117605E-4	0.0	1.243706843372535E-4	0.0
15	0.0	3.731120530117605E-4	0.0	4.97482737349014E-4	0.0
16	0.0	4.97482737349014E-4	0.0	0.0011193361590352815	0.0
17	0.0	4.97482737349014E-4	0.0	0.0013680775277097885	0.0
18	0.0	4.97482737349014E-4	0.0	0.0013680775277097885	0.0
19	0.0	4.97482737349014E-4	0.0	0.001492448212047042	0.0
20	0.0	4.97482737349014E-4	0.0	0.0023630430024078166	0.0
21	0.0	4.97482737349014E-4	0.0	0.0026117843710823235	0.0
22	0.0	4.97482737349014E-4	0.0	0.0031092671084313375	0.0
23	0.0	4.97482737349014E-4	0.0	0.005099198057827393	0.0
24	0.0	6.218534216862675E-4	0.0	0.008084094481921477	0.0
25	0.0	6.218534216862675E-4	0.0	0.010944620221678308	1.243706843372535E-4
26	0.0	6.218534216862675E-4	0.0	0.018033749228901757	1.243706843372535E-4
27	0.0	6.218534216862675E-4	0.0	0.054225618371042526	1.243706843372535E-4
28	0.0	7.46224106023521E-4	0.0	0.18456609555648418	1.243706843372535E-4
29	0.0	7.46224106023521E-4	0.0	0.35482956241418423	1.243706843372535E-4
30	0.0	7.46224106023521E-4	0.0	0.5697421049489583	1.243706843372535E-4
31	0.0	7.46224106023521E-4	0.0	1.077423238413627	1.243706843372535E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	945	0.0	25.058199	1
GGTATAC	40	0.0019306855	23.125	3
TAGAACA	65	2.6811813E-6	22.76923	4
TCGTTTA	170	0.0	21.764706	30
TTAGAAC	70	5.0987783E-6	21.142857	3
CGTTTAT	170	0.0	20.676472	31
GCTAAAC	45	0.0038248224	20.555557	3
ATGGTCG	150	0.0	19.733334	36
CAGTCGG	265	0.0	18.849056	10
GTATTAC	70	1.2186194E-4	18.5	1
TAGTACT	50	0.0070330896	18.499998	4
AGTCGGT	275	0.0	18.163637	11
ATCAGTA	75	2.066293E-4	17.266666	2
GTATCAA	1405	0.0	16.985765	2
CCAATAC	100	5.873373E-6	16.65	3
CTCTATG	425	0.0	16.541178	1
GTACTAT	90	4.443282E-5	16.444445	1
TGGTCGG	180	1.0913936E-11	16.444445	37
GCAGTCG	305	0.0	16.37705	9
TATGGTC	205	0.0	16.243904	35
>>END_MODULE