FastQCFastQC Report
Fri 10 Feb 2017
ERR1631267.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631267.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1115028
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT49250.4416929440336924No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT40310.36151558525884553No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT34860.31263788891400035No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17120.15353874521536678No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA15140.1357813436075148No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC12620.11318101428843043No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC11850.1062753581075991No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC11820.10600630656808618No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCAATA450.00382573820.5555552
TCGTAGC555.1429606E-420.1818182
TATTAGA851.2452401E-619.5882342
TCTATAC851.2452401E-619.5882343
CTATACA701.2191559E-418.54
GTTAATA609.236579E-418.52
TGCTAGC500.00703475218.52
GGTATCA24350.018.4620111
CTTATAC19550.018.16879837
GCAGTCG7250.017.3517239
CAGTCGG7250.017.35172310
TATACAC6550.016.94656637
TCGGTGA7350.016.86394713
AGTCGGT7400.016.7511
GGCAGTC7900.016.3924058
CCGCTTA2150.016.34883725
CGCTTAT2150.016.34883726
CTCTATG11700.016.2863251
TCTAGCG3650.016.21917728
TTAATAC803.3824524E-416.18753
Produced by FastQC (version 0.11.5)