##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631267.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1115028
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.066024350958	34.0	33.0	34.0	31.0	34.0
2	33.20893735403954	34.0	33.0	34.0	31.0	34.0
3	33.289263587999585	34.0	34.0	34.0	31.0	34.0
4	36.51251448394121	37.0	37.0	37.0	35.0	37.0
5	36.501309384158965	37.0	37.0	37.0	35.0	37.0
6	36.553127813830685	37.0	37.0	37.0	35.0	37.0
7	36.56136437829364	37.0	37.0	37.0	35.0	37.0
8	36.56303070416169	37.0	37.0	37.0	35.0	37.0
9	38.409008563013664	39.0	39.0	39.0	37.0	39.0
10	38.38421905100141	39.0	39.0	39.0	37.0	39.0
11	38.42822422396568	39.0	39.0	39.0	37.0	39.0
12	38.39288251057373	39.0	39.0	39.0	37.0	39.0
13	38.41586848043278	39.0	39.0	39.0	37.0	39.0
14	39.96410673095205	41.0	40.0	41.0	38.0	41.0
15	39.95237249647543	41.0	40.0	41.0	38.0	41.0
16	39.91039686895755	41.0	40.0	41.0	38.0	41.0
17	39.909248915722294	41.0	40.0	41.0	38.0	41.0
18	39.891259232951995	41.0	40.0	41.0	38.0	41.0
19	39.909606754269845	41.0	40.0	41.0	38.0	41.0
20	39.877273933928116	41.0	40.0	41.0	38.0	41.0
21	39.84982170851315	41.0	40.0	41.0	38.0	41.0
22	39.783674490685435	41.0	40.0	41.0	38.0	41.0
23	39.72366613215094	41.0	40.0	41.0	37.0	41.0
24	39.6930112965773	41.0	40.0	41.0	37.0	41.0
25	39.68617380011982	41.0	40.0	41.0	37.0	41.0
26	39.58571533629649	41.0	40.0	41.0	37.0	41.0
27	39.53002615180964	41.0	40.0	41.0	37.0	41.0
28	39.444500048429276	41.0	39.0	41.0	36.0	41.0
29	39.38404506433919	41.0	39.0	41.0	36.0	41.0
30	39.30544076023203	41.0	39.0	41.0	35.0	41.0
31	39.239650484113405	41.0	39.0	41.0	35.0	41.0
32	39.15400958540951	41.0	39.0	41.0	35.0	41.0
33	39.06780726582651	41.0	39.0	41.0	35.0	41.0
34	39.00481781623421	41.0	39.0	41.0	35.0	41.0
35	38.89964825995401	41.0	39.0	41.0	35.0	41.0
36	38.82364030320315	41.0	38.0	41.0	35.0	41.0
37	38.744084453484575	40.0	38.0	41.0	35.0	41.0
38	38.6520114293094	40.0	38.0	41.0	35.0	41.0
39	38.545289445646205	40.0	38.0	41.0	35.0	41.0
40	38.42950221877836	40.0	38.0	41.0	35.0	41.0
41	38.313239667524044	40.0	37.0	41.0	35.0	41.0
42	38.150070670871045	40.0	37.0	41.0	35.0	41.0
43	37.34889796489416	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	0.0
12	2.0
13	3.0
14	4.0
15	3.0
16	7.0
17	8.0
18	21.0
19	29.0
20	62.0
21	132.0
22	250.0
23	379.0
24	649.0
25	1040.0
26	1587.0
27	2260.0
28	3348.0
29	4609.0
30	6206.0
31	7793.0
32	10167.0
33	13271.0
34	19849.0
35	29750.0
36	47493.0
37	97368.0
38	204810.0
39	663925.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.41532230580757	17.446916131254103	12.602732846170678	26.53502871676765
2	19.913042542429427	19.559867554895483	33.07199460461979	27.455095298055294
3	20.403792550500974	21.5069038625039	28.271666720476972	29.81763686651815
4	14.908773591335823	15.607859174836866	32.59353128351934	36.889835950307976
5	16.61967233109841	34.48648823168566	31.71095255007049	17.182886887145436
6	34.880290001686056	33.038004426794664	15.986863110164048	16.09484246135523
7	30.827566662003107	29.047252625046188	20.10191672316749	20.023263989783217
8	29.169760759371066	30.87680309373397	19.024006572032274	20.92942957486269
9	27.803158306338492	13.933641128294536	19.03440989822677	39.228790667140196
10	19.653049071413452	24.544675111297654	29.272359079771988	26.529916737516906
11	37.66685679642126	21.09982888322087	19.56193028336508	21.671384036992794
12	23.834827466216097	24.850945447109847	26.356737229917098	24.957489856756958
13	31.519029118551284	17.865650010582694	23.974734266762805	26.640586604103216
14	24.109349720365767	19.821565018995038	23.313405582640076	32.75567967799912
15	27.66011257116413	25.676305886488947	20.66853926538168	25.99504227696524
16	26.710988423609095	24.338940367416782	22.929558719601662	26.020512489372464
17	25.446984290977447	25.254612440225717	23.48828908332347	25.81011418547337
18	25.74051952058603	22.747590195044427	25.31550777200214	26.1963825123674
19	25.857377572581136	24.3956205584075	24.730589725101073	25.016412143910287
20	26.2025706977762	22.95897502125507	24.808524987713312	26.029929293255417
21	27.984319676277185	23.343808406605035	24.109977507291298	24.56189440982648
22	27.57311924005496	22.924626108043924	23.659405862453678	25.84284878944744
23	25.828858109392765	23.144351531979463	24.43866880472957	26.588121553898198
24	25.44788112944249	24.177509443709035	24.430866310083694	25.94374311676478
25	25.805450625455144	23.033233246160634	24.346742862062655	26.814573266321563
26	25.926972237468476	24.187733402210526	24.53167095355453	25.353623406766467
27	26.442564671021714	23.064263857051124	24.09320662799499	26.39996484393217
28	25.09730697345717	23.65545977320749	25.31075452813741	25.93647872519793
29	24.849600189412282	23.796980882991278	25.7044666142913	25.648952313305134
30	24.396517396872543	24.49400373802272	25.757290399882333	25.352188465222397
31	25.673705054940328	24.084776346423588	24.118945891941728	26.122572706694363
32	24.59938225766528	23.622814853079923	25.029954404732436	26.747848484522365
33	24.353110415164462	23.050004125456937	25.939707343672087	26.657178115706508
34	25.696664119645423	22.83763277693475	25.320081648173858	26.145621455245966
35	25.33927399132578	22.851533773142915	26.07773078344221	25.731461452089093
36	23.78218304831807	23.402551326065353	26.06795524417324	26.747310381443334
37	24.529518541238428	22.09684420480921	26.824438489437036	26.54919876451533
38	23.601828832997914	21.362961288864497	28.000283400954956	27.034926477182637
39	23.86056673016283	21.113640195582533	28.421528427985663	26.604264646268973
40	23.167131228991558	21.652819480766404	28.79622753868064	26.3838217515614
41	21.9299425664647	21.94294672420782	29.935929860057325	26.19118084927015
42	22.520421011849027	22.180429549751217	29.528585829234782	25.77056360916497
43	21.053462334578146	21.699186029409127	29.904450829934316	27.34290080607841
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	67.0
1	99.0
2	131.0
3	422.0
4	713.0
5	713.0
6	960.0
7	1207.0
8	1166.5
9	1126.0
10	1808.0
11	2490.0
12	2490.0
13	4191.5
14	5893.0
15	8255.5
16	10618.0
17	9291.0
18	7964.0
19	7964.0
20	8569.0
21	9174.0
22	7045.5
23	4917.0
24	5276.0
25	5635.0
26	5635.0
27	6495.5
28	7356.0
29	9166.5
30	10977.0
31	13546.0
32	16115.0
33	16115.0
34	20031.5
35	23948.0
36	29198.0
37	34448.0
38	39988.5
39	45529.0
40	45529.0
41	49932.0
42	54335.0
43	59127.5
44	63920.0
45	69076.0
46	74232.0
47	74232.0
48	80489.0
49	86746.0
50	87296.5
51	87847.0
52	87317.5
53	86788.0
54	86788.0
55	83680.0
56	80572.0
57	77986.0
58	75400.0
59	73416.5
60	71433.0
61	71433.0
62	66934.5
63	62436.0
64	57669.0
65	52902.0
66	47304.0
67	41706.0
68	41706.0
69	35894.5
70	30083.0
71	26147.5
72	22212.0
73	17229.5
74	12247.0
75	12247.0
76	10075.5
77	7904.0
78	6583.0
79	5262.0
80	4703.5
81	4145.0
82	4145.0
83	3290.0
84	2435.0
85	2201.5
86	1968.0
87	1666.5
88	1365.0
89	1365.0
90	992.5
91	620.0
92	366.0
93	112.0
94	74.5
95	37.0
96	37.0
97	23.0
98	9.0
99	6.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1115028.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.82511108041918
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.795967329283	49.01219628570077
2	6.575155934337894	7.341176208109807
3	1.9571614913946944	3.2777627297828307
4	0.9318835759376707	2.080900165629548
5	0.5576373967340558	1.5565084807637222
6	0.348756471017186	1.168162124072964
7	0.25729113519658203	1.0054314341649437
8	0.19086054073054087	0.852384870972105
9	0.14252563222997416	0.7160858325940753
>10	0.9962730621383361	11.102806861196694
>50	0.13222493217017411	5.20682786907565
>100	0.10343422869174448	11.510741354978252
>500	0.00937372638810316	3.3298342788112603
>1k	0.0014545437498780765	1.8391815041473851
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4925	0.4416929440336924	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4031	0.36151558525884553	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3486	0.31263788891400035	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1712	0.15353874521536678	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1514	0.1357813436075148	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	1262	0.11318101428843043	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	1185	0.1062753581075991	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	1182	0.10600630656808618	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	8.968384650430303E-5	0.0	0.0	0.0
3	0.0	8.968384650430303E-5	0.0	0.0	0.0
4	0.0	8.968384650430303E-5	0.0	0.0	0.0
5	0.0	8.968384650430303E-5	0.0	0.0	0.0
6	0.0	8.968384650430303E-5	0.0	0.0	0.0
7	0.0	8.968384650430303E-5	0.0	0.0	0.0
8	0.0	8.968384650430303E-5	0.0	0.0	0.0
9	0.0	8.968384650430303E-5	0.0	0.0	0.0
10	0.0	8.968384650430303E-5	0.0	0.0	0.0
11	0.0	1.7936769300860606E-4	0.0	0.0	0.0
12	0.0	1.7936769300860606E-4	0.0	3.587353860172121E-4	0.0
13	0.0	1.7936769300860606E-4	0.0	4.4841923252151515E-4	0.0
14	0.0	1.7936769300860606E-4	0.0	7.174707720344242E-4	0.0
15	0.0	1.7936769300860606E-4	0.0	0.0014349415440688485	0.0
16	0.0	1.7936769300860606E-4	0.0	0.0041254569391979395	0.0
17	0.0	1.7936769300860606E-4	0.0	0.006995340027335636	0.0
18	0.0	1.7936769300860606E-4	0.0	0.008430281571404486	0.0
19	0.0	1.7936769300860606E-4	0.0	0.010582693887507757	0.0
20	0.0	1.7936769300860606E-4	0.0	0.011927951585072304	0.0
21	0.0	1.7936769300860606E-4	0.0	0.01417004774767988	0.0
22	0.0	1.7936769300860606E-4	0.0	0.017936769300860606	0.0
23	0.0	1.7936769300860606E-4	0.0	0.02286938085859727	0.0
24	0.0	1.7936769300860606E-4	0.0	0.033362390899600726	0.0
25	0.0	1.7936769300860606E-4	0.0	0.038922789382867515	0.0
26	0.0	1.7936769300860606E-4	0.0	0.05165789558647855	0.0
27	0.0	1.7936769300860606E-4	0.0	0.10439199733100873	0.0
28	0.0	1.7936769300860606E-4	0.0	0.29730195116176455	0.0
29	0.0	2.690515395129091E-4	0.0	0.5459055736716926	0.0
30	0.0	3.587353860172121E-4	0.0	0.8853589326904795	0.0
31	0.0	3.587353860172121E-4	0.0	1.7228266913476613	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCAATA	45	0.003825738	20.555555	2
TCGTAGC	55	5.1429606E-4	20.181818	2
TATTAGA	85	1.2452401E-6	19.588234	2
TCTATAC	85	1.2452401E-6	19.588234	3
CTATACA	70	1.2191559E-4	18.5	4
GTTAATA	60	9.236579E-4	18.5	2
TGCTAGC	50	0.007034752	18.5	2
GGTATCA	2435	0.0	18.462011	1
CTTATAC	1955	0.0	18.168798	37
GCAGTCG	725	0.0	17.351723	9
CAGTCGG	725	0.0	17.351723	10
TATACAC	655	0.0	16.946566	37
TCGGTGA	735	0.0	16.863947	13
AGTCGGT	740	0.0	16.75	11
GGCAGTC	790	0.0	16.392405	8
CCGCTTA	215	0.0	16.348837	25
CGCTTAT	215	0.0	16.348837	26
CTCTATG	1170	0.0	16.286325	1
TCTAGCG	365	0.0	16.219177	28
TTAATAC	80	3.3824524E-4	16.1875	3
>>END_MODULE