FastQCFastQC Report
Fri 10 Feb 2017
ERR1631266.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631266.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences235875
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA4670.19798622151563328No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC3560.1509273979862215No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC3350.14202437731849496No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC3250.1377848436671966No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC2960.12549019607843137No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC2850.12082670906200317No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC2770.1174350821409645No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTCTGTC2480.10514043455219926No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCTA250.005487599429.616
GTAGACC250.005487599429.626
TTAATAT358.8484236E-426.428573
ATATACG358.8484236E-426.428576
TATACGC358.8484236E-426.428577
ATACACA358.8484236E-426.4285737
GGTATCA1150.025.739131
GTAATGT400.001926664423.1254
TACGCTA400.001926664423.1259
ATACGCT400.001926664423.1258
TAATATA502.693288E-422.24
GCGCAAG806.911978E-720.81251
CGTATTG450.003816932420.55555727
TTACTAC450.003816932420.55555725
AATATAC450.003816932420.5555575
AAGACGG555.126454E-420.1818185
AAGCATT555.126454E-420.18181822
AGGGACG555.126454E-420.1818188
ACCGCAG656.872448E-519.92307729
ATACCGC656.872448E-519.92307727