##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631266.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	235875
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.045426603073665	34.0	33.0	34.0	31.0	34.0
2	33.19364069952305	34.0	33.0	34.0	31.0	34.0
3	33.27577742448331	34.0	34.0	34.0	31.0	34.0
4	36.51204663487017	37.0	37.0	37.0	35.0	37.0
5	36.50001483836778	37.0	37.0	37.0	35.0	37.0
6	36.555357710651826	37.0	37.0	37.0	35.0	37.0
7	36.55415792262851	37.0	37.0	37.0	35.0	37.0
8	36.55370853206147	37.0	37.0	37.0	35.0	37.0
9	38.40964917859036	39.0	39.0	39.0	37.0	39.0
10	38.36228510863805	39.0	39.0	39.0	37.0	39.0
11	38.428845786963436	39.0	39.0	39.0	37.0	39.0
12	38.36597350291468	39.0	39.0	39.0	37.0	39.0
13	38.39848648648648	39.0	39.0	39.0	37.0	39.0
14	39.93897615262321	41.0	40.0	41.0	38.0	41.0
15	39.94819289878114	41.0	40.0	41.0	38.0	41.0
16	39.92803815580286	41.0	40.0	41.0	38.0	41.0
17	39.897937466878645	41.0	40.0	41.0	38.0	41.0
18	39.869413884472706	41.0	40.0	41.0	38.0	41.0
19	39.88383677795443	41.0	40.0	41.0	38.0	41.0
20	39.86179968203498	41.0	40.0	41.0	38.0	41.0
21	39.8244875463699	41.0	40.0	41.0	38.0	41.0
22	39.7732315845257	41.0	40.0	41.0	38.0	41.0
23	39.71222469528352	41.0	40.0	41.0	37.0	41.0
24	39.6783804981452	41.0	40.0	41.0	37.0	41.0
25	39.65352835188129	41.0	40.0	41.0	37.0	41.0
26	39.592237413884476	41.0	40.0	41.0	37.0	41.0
27	39.52710969793323	41.0	40.0	41.0	37.0	41.0
28	39.43803709591945	41.0	39.0	41.0	37.0	41.0
29	39.385305776364596	41.0	39.0	41.0	36.0	41.0
30	39.32353153153153	41.0	39.0	41.0	36.0	41.0
31	39.28788977212507	41.0	39.0	41.0	35.0	41.0
32	39.218535241123476	41.0	39.0	41.0	35.0	41.0
33	39.1440084790673	41.0	39.0	41.0	35.0	41.0
34	39.09261261261261	41.0	39.0	41.0	35.0	41.0
35	38.99341176470588	41.0	39.0	41.0	35.0	41.0
36	38.927503974562796	40.0	39.0	41.0	35.0	41.0
37	38.87680339162692	40.0	38.0	41.0	35.0	41.0
38	38.81344356120827	40.0	38.0	41.0	35.0	41.0
39	38.725647058823526	40.0	38.0	41.0	35.0	41.0
40	38.63388659247483	40.0	38.0	41.0	35.0	41.0
41	38.561195548489664	40.0	37.0	41.0	35.0	41.0
42	38.441326974032854	40.0	37.0	41.0	35.0	41.0
43	37.624089030206676	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	0.0
16	0.0
17	1.0
18	4.0
19	9.0
20	8.0
21	37.0
22	47.0
23	91.0
24	124.0
25	184.0
26	343.0
27	384.0
28	577.0
29	801.0
30	1018.0
31	1350.0
32	1810.0
33	2486.0
34	4213.0
35	6313.0
36	10371.0
37	19630.0
38	48100.0
39	137973.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.58113407525172	17.358770535241124	11.470482246952836	27.58961314255432
2	21.26507684154743	18.59162692103869	30.995654478007417	29.14764175940647
3	20.757604663487015	19.9800741918389	28.21706412294648	31.04525702172761
4	15.27970323264441	14.841759406465288	32.63084260731319	37.24769475357711
5	17.34054054054054	33.65341812400636	32.37647058823529	16.629570747217805
6	37.11881293057764	33.1879173290938	14.540328563857976	15.152941176470588
7	32.613036565977744	27.61547429782724	19.35855855855856	20.41293057763646
8	30.30079491255962	29.37276099629041	18.34531001589825	21.981134075251724
9	28.095389507154213	12.947111817700051	17.75601483836778	41.20148383677795
10	19.79014308426073	23.138526762056173	29.756438791732908	27.314891361950185
11	40.82289348171701	19.20084790673026	18.739586645468997	21.23667196608373
12	24.888606253312133	23.10630630630631	25.011128775834656	26.993958664546902
13	33.27821939586645	16.1492315845257	22.278325384207736	28.294223635400108
14	25.11245363010069	18.14817170111288	21.176470588235293	35.562904080551135
15	28.908532061473235	24.406147323794382	19.121992580816112	27.563328033916267
16	29.074297827239004	22.457657657657656	20.715633280339162	27.752411234764175
17	27.872813990461047	22.616640169581345	21.4223635400106	28.088182299947007
18	27.73672496025437	20.95050344462109	22.895601483836778	28.41717011128776
19	28.198410174880763	22.03879173290938	21.921780604133545	27.841017488076314
20	28.337890832008476	20.942448330683625	21.732697403285638	28.986963434022258
21	30.079067302596712	21.827662957074722	21.272284048754635	26.820985691573927
22	30.076947535771065	21.416852146263913	20.93057763645999	27.575622681505035
23	28.39809220985692	21.00858505564388	21.767461579226286	28.825861155272918
24	27.555696873343933	22.16894541600424	21.39099099099099	28.884366719660836
25	28.15601483836778	20.627874933757287	21.75135135135135	29.464758876523582
26	27.949549549549552	22.19353471118177	21.846740858505566	28.010174880763117
27	28.78600953895071	21.03402225755167	21.850980392156863	28.32898781134075
28	27.69263381028087	21.610174880763118	22.53142554319025	28.165765765765766
29	26.98590355060943	21.913301536830947	23.334393216746157	27.766401695813457
30	26.344462109167992	22.88415474297827	22.826497085320614	27.94488606253312
31	28.117011128775836	22.434340222575518	21.44144144144144	28.00720720720721
32	26.806147323794384	22.078219395866455	22.198622151563328	28.917011128775833
33	25.936618971913088	21.62798092209857	23.3009009009009	29.134499205087437
34	27.775304716481187	21.20699523052464	23.731213566507684	27.28648648648649
35	27.19533651298357	21.24133545310016	24.165341812400637	27.397986221515637
36	25.922628510863806	22.384737678855327	23.678643349231585	28.013990461049282
37	26.678961314255435	20.72156862745098	24.606677265500796	27.99279279279279
38	25.196820349761527	20.401059883412824	25.86157922628511	28.540540540540544
39	25.539798622151565	19.91690514043455	26.374986751457342	28.168309485956545
40	24.559618441971384	20.01865394806571	27.53704292527822	27.884684684684686
41	23.810068892421835	19.885532591414943	28.869104398516164	27.435294117647057
42	23.882564917859035	19.71679915209327	29.414308426073134	26.98632750397456
43	22.199894011658717	18.624271330153682	29.805617382087966	29.37021727609963
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	2.0
2	2.0
3	3.5
4	5.0
5	5.0
6	10.0
7	15.0
8	13.0
9	11.0
10	18.0
11	25.0
12	25.0
13	45.0
14	65.0
15	97.5
16	130.0
17	125.0
18	120.0
19	120.0
20	150.0
21	180.0
22	189.0
23	198.0
24	262.0
25	326.0
26	326.0
27	448.0
28	570.0
29	834.5
30	1099.0
31	1499.0
32	1899.0
33	1899.0
34	2602.5
35	3306.0
36	4317.0
37	5328.0
38	6357.5
39	7387.0
40	7387.0
41	8643.5
42	9900.0
43	11172.0
44	12444.0
45	13417.5
46	14391.0
47	14391.0
48	15725.5
49	17060.0
50	17632.5
51	18205.0
52	19259.5
53	20314.0
54	20314.0
55	19785.5
56	19257.0
57	18970.5
58	18684.0
59	18678.0
60	18672.0
61	18672.0
62	18007.5
63	17343.0
64	16130.5
65	14918.0
66	13263.0
67	11608.0
68	11608.0
69	9832.5
70	8057.0
71	6862.0
72	5667.0
73	4339.5
74	3012.0
75	3012.0
76	2431.0
77	1850.0
78	1627.5
79	1405.0
80	1227.5
81	1050.0
82	1050.0
83	854.0
84	658.0
85	509.0
86	360.0
87	273.0
88	186.0
89	186.0
90	153.0
91	120.0
92	75.0
93	30.0
94	22.0
95	14.0
96	14.0
97	7.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	235875.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.15530898769393
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.40337780069088	44.33332317613095
2	8.073256212629474	8.582728570382786
3	2.6074926065689996	4.158062255559079
4	1.3952905004632081	2.966683907188637
5	0.8915667189886317	2.36957522154926
6	0.5844152742550772	1.8638864688093912
7	0.42245084561314017	1.5718853661475334
8	0.30755749165146967	1.3078650800171172
9	0.2659832868820307	1.2724581419799101
>10	1.7994550680590058	18.704906565771022
>50	0.1720350388510623	6.2414356541293
>100	0.07711915534702793	6.627189592335
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	467	0.19798622151563328	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	356	0.1509273979862215	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	335	0.14202437731849496	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	325	0.1377848436671966	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC	296	0.12549019607843137	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	285	0.12082670906200317	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	277	0.1174350821409645	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTCTGTC	248	0.10514043455219926	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	4.2395336512983573E-4	0.0
17	0.0	0.0	0.0	4.2395336512983573E-4	0.0
18	0.0	0.0	0.0	4.2395336512983573E-4	0.0
19	0.0	0.0	0.0	4.2395336512983573E-4	0.0
20	0.0	0.0	0.0	4.2395336512983573E-4	0.0
21	0.0	0.0	0.0	0.001695813460519343	0.0
22	0.0	0.0	0.0	0.0025437201907790143	0.0
23	0.0	0.0	0.0	0.003391626921038686	0.0
24	0.0	0.0	0.0	0.0038155802861685214	0.0
25	0.0	0.0	0.0	0.007207207207207207	0.0
26	0.0	0.0	0.0	0.011446740858505564	0.0
27	0.0	0.0	0.0	0.03476417594064653	0.0
28	0.0	0.0	0.0	0.14160042395336514	0.0
29	0.0	0.0	0.0	0.3077901430842607	0.0
30	0.0	0.0	0.0	0.5151033386327504	0.0
31	0.0	0.0	0.0	1.0526762056173822	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGCTA	25	0.0054875994	29.6	16
GTAGACC	25	0.0054875994	29.6	26
TTAATAT	35	8.8484236E-4	26.42857	3
ATATACG	35	8.8484236E-4	26.42857	6
TATACGC	35	8.8484236E-4	26.42857	7
ATACACA	35	8.8484236E-4	26.42857	37
GGTATCA	115	0.0	25.73913	1
GTAATGT	40	0.0019266644	23.125	4
TACGCTA	40	0.0019266644	23.125	9
ATACGCT	40	0.0019266644	23.125	8
TAATATA	50	2.693288E-4	22.2	4
GCGCAAG	80	6.911978E-7	20.8125	1
CGTATTG	45	0.0038169324	20.555557	27
TTACTAC	45	0.0038169324	20.555557	25
AATATAC	45	0.0038169324	20.555557	5
AAGACGG	55	5.126454E-4	20.181818	5
AAGCATT	55	5.126454E-4	20.181818	22
AGGGACG	55	5.126454E-4	20.181818	8
ACCGCAG	65	6.872448E-5	19.923077	29
ATACCGC	65	6.872448E-5	19.923077	27
>>END_MODULE