FastQCFastQC Report
Fri 10 Feb 2017
ERR1631265.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631265.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences492178
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT53631.089646428731069No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT39880.8102759570724414No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT37290.7576527191381979No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14400.29257707577339903No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA12870.2614907614724754No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA8610.17493670988951152No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC7360.14953939428418175No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC6810.13836457541783664No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC6720.13653596869425288No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC6690.13592643311972497No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA6270.12739293507633417No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA5850.11885943703294337No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG5820.11824990145841545No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT5780.11743718735904489No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT4990.10138608389647648No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACT604.3122782E-827.7499984
TCTAATA400.001929627123.1252
CGCCGAA400.001929627123.12514
TAGATGC502.6990613E-422.19999937
AATACTG502.6990613E-422.1999995
AAGACGG1950.021.8205135
AGACGGA2050.021.6585376
GTTCTAG603.7207174E-521.5833321
CAAGACG1850.021.04
TTAGACT450.00382274720.5555554
GTCTTGG555.137353E-420.1818181
AGTCGGT7750.019.57419211
GACGGAC2100.019.380957
GCGCAAG2200.019.3409081
CGCAAGA2200.019.3409082
GCAGTCG8000.019.1937489
GGCAGTC8150.019.0674848
AGGACCG2150.018.9302335
ACGGACC2150.018.9302338
CAGTCGG8150.018.8404910
Produced by FastQC (version 0.11.5)