##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631265.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	492178
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.0464831829135	34.0	33.0	34.0	31.0	34.0
2	33.171718362056005	34.0	33.0	34.0	31.0	34.0
3	33.23813742182706	34.0	33.0	34.0	31.0	34.0
4	36.484085026149074	37.0	37.0	37.0	35.0	37.0
5	36.484410111788826	37.0	37.0	37.0	35.0	37.0
6	36.54693017566815	37.0	37.0	37.0	35.0	37.0
7	36.554368541462644	37.0	37.0	37.0	35.0	37.0
8	36.56623416731345	37.0	37.0	37.0	35.0	37.0
9	38.40338861143733	39.0	39.0	39.0	37.0	39.0
10	38.3704269593521	39.0	39.0	39.0	37.0	39.0
11	38.42432412663711	39.0	39.0	39.0	37.0	39.0
12	38.391553462365245	39.0	39.0	39.0	37.0	39.0
13	38.40961603322375	39.0	39.0	39.0	37.0	39.0
14	39.917302276818546	41.0	40.0	41.0	38.0	41.0
15	39.90110691660334	41.0	40.0	41.0	38.0	41.0
16	39.85228311708366	41.0	40.0	41.0	38.0	41.0
17	39.86288903608044	41.0	40.0	41.0	38.0	41.0
18	39.87817415650436	41.0	40.0	41.0	38.0	41.0
19	39.90273031301683	41.0	40.0	41.0	38.0	41.0
20	39.8874817647274	41.0	40.0	41.0	38.0	41.0
21	39.868216377001815	41.0	40.0	41.0	38.0	41.0
22	39.79363157231733	41.0	40.0	41.0	38.0	41.0
23	39.721003376827085	41.0	40.0	41.0	37.0	41.0
24	39.68539227677791	41.0	40.0	41.0	37.0	41.0
25	39.68155220265839	41.0	40.0	41.0	37.0	41.0
26	39.57819528707094	41.0	40.0	41.0	37.0	41.0
27	39.500190987813355	41.0	40.0	41.0	36.0	41.0
28	39.39066354042643	41.0	39.0	41.0	36.0	41.0
29	39.31200500631885	41.0	39.0	41.0	35.0	41.0
30	39.19611400753386	41.0	39.0	41.0	35.0	41.0
31	39.089508267334175	41.0	39.0	41.0	35.0	41.0
32	38.97134776442669	41.0	38.0	41.0	35.0	41.0
33	38.84966617768368	41.0	38.0	41.0	35.0	41.0
34	38.767271190504246	40.0	38.0	41.0	35.0	41.0
35	38.62968682062181	40.0	38.0	41.0	35.0	41.0
36	38.536909410822915	40.0	38.0	41.0	35.0	41.0
37	38.426412801872495	40.0	38.0	41.0	35.0	41.0
38	38.31643836173092	40.0	37.0	41.0	35.0	41.0
39	38.16766698227064	40.0	37.0	41.0	35.0	41.0
40	38.01100008533498	40.0	37.0	41.0	34.0	41.0
41	37.85169999471736	40.0	37.0	41.0	34.0	41.0
42	37.64400277948222	40.0	36.0	41.0	34.0	41.0
43	36.81592025649257	39.0	35.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	3.0
13	2.0
14	2.0
15	4.0
16	0.0
17	6.0
18	5.0
19	13.0
20	36.0
21	56.0
22	139.0
23	204.0
24	307.0
25	510.0
26	718.0
27	1153.0
28	1661.0
29	2380.0
30	3205.0
31	3986.0
32	4794.0
33	6300.0
34	9508.0
35	14023.0
36	22580.0
37	51911.0
38	93077.0
39	275592.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.88294885183816	16.644994290683453	12.787853175070808	25.684203682407585
2	19.490915888154287	20.383275969263153	32.8415735770392	27.284234565543358
3	20.1018737123561	22.587559785280938	29.260552076687702	28.05001442567526
4	13.495930334147403	15.772748883534005	33.54700941529284	37.18431136702575
5	15.082551434643566	37.08698885362613	31.563783834303848	16.266675877426458
6	33.156500290545296	33.762175473101195	17.12896553685862	15.952358699494898
7	30.56556774175197	30.350198505418774	19.606524468789747	19.477709284039516
8	31.865910300744854	29.103088719934654	18.644270975135015	20.386730004185477
9	28.96777182238946	13.133663024352979	18.440482914717844	39.45808223853972
10	19.476490212890457	25.71874403163083	28.200163355533974	26.60460239994474
11	36.927493711624656	22.580448536911444	18.583520596207062	21.908537155256838
12	24.21501976927047	26.507686243594797	25.863813498368476	23.413480488766258
13	32.25133996237134	17.86467497531381	24.49865698995079	25.38532807236406
14	23.868194027364083	20.21646640036735	24.141062786227746	31.774276786040822
15	28.230436955735527	25.969059974236963	20.91763548959929	24.88286758042822
16	25.922532092048	24.670342843442818	23.794643401371047	25.61248166313813
17	24.332863313679198	26.204950241579265	24.033987703635677	25.428198741105863
18	25.109005278578078	22.450414281012154	26.737481155191823	25.703099285217952
19	25.009447801405184	25.17463194210225	26.396953947555556	23.418966308937012
20	26.01883871282341	22.49308177122911	26.54852512708817	24.939554388859314
21	28.207274603903464	22.8451901547814	25.71630588933272	23.231229351982414
22	27.54450625586678	22.565819683122772	24.786967316702494	25.10270674430795
23	25.002946088610216	23.180231542246908	25.504797044971532	26.312025324171334
24	24.028095526415242	24.332863313679198	26.793558428048385	24.845482731857174
25	24.363746449455277	23.45817976423164	25.710616890637127	26.467456895675955
26	25.231318750533344	24.331034706955617	25.75998927217389	24.677657270337157
27	25.657180938603513	23.01078065252815	25.441608523745472	25.890429885122863
28	23.896232663792368	24.003714103434124	26.83378777596723	25.266265456806273
29	23.577242379789425	24.25362368899057	27.618666417434344	24.550467513785662
30	22.998996298087278	25.242493569399688	27.94273616455835	23.815773967954684
31	24.128059360637817	25.55884253257968	25.5141432571143	24.79895484966821
32	23.418356773362483	24.592322289903244	26.897992189817504	25.091328746916762
33	23.161335939436544	24.000666425561484	27.515654905339126	25.322342729662843
34	24.96291991921622	24.154066211817675	26.086497161596007	24.7965167073701
35	24.088642726818346	24.586226934157967	26.81590806578108	24.509222273242607
36	22.127157247987515	25.646818833836537	27.016648448325604	25.209375469850336
37	23.66745364481956	24.315593139067573	27.20824579725221	24.808707418860656
38	22.85453636692416	23.018095079422483	28.373474637224742	25.75389391642861
39	23.660342396450066	22.84844101117888	28.127831800689997	25.36338479168106
40	22.605845852516772	23.161335939436544	28.248113487396836	25.984704720649848
41	20.915603704350865	23.738972485564165	29.858709653824427	25.486714156260543
42	22.14747510047178	23.156866011890006	29.705716224617923	24.98994266302029
43	20.736806602489345	23.110338129701045	30.020033402549483	26.13282186526013
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	24.0
1	43.5
2	63.0
3	255.0
4	447.0
5	447.0
6	615.0
7	783.0
8	780.0
9	777.0
10	1182.5
11	1588.0
12	1588.0
13	2842.0
14	4096.0
15	6813.5
16	9531.0
17	8371.0
18	7211.0
19	7211.0
20	7842.0
21	8473.0
22	6287.0
23	4101.0
24	4159.0
25	4217.0
26	4217.0
27	4470.0
28	4723.0
29	5561.0
30	6399.0
31	7234.0
32	8069.0
33	8069.0
34	9397.0
35	10725.0
36	12651.5
37	14578.0
38	16243.0
39	17908.0
40	17908.0
41	19591.5
42	21275.0
43	22915.5
44	24556.0
45	27600.5
46	30645.0
47	30645.0
48	35293.0
49	39941.0
50	39268.5
51	38596.0
52	36896.0
53	35196.0
54	35196.0
55	33149.5
56	31103.0
57	30393.5
58	29684.0
59	29385.0
60	29086.0
61	29086.0
62	27758.0
63	26430.0
64	25047.0
65	23664.0
66	21350.0
67	19036.0
68	19036.0
69	16585.5
70	14135.0
71	12110.5
72	10086.0
73	7571.5
74	5057.0
75	5057.0
76	4116.0
77	3175.0
78	2687.0
79	2199.0
80	1951.0
81	1703.0
82	1703.0
83	1397.5
84	1092.0
85	955.0
86	818.0
87	717.0
88	616.0
89	616.0
90	457.5
91	299.0
92	169.0
93	39.0
94	32.0
95	25.0
96	25.0
97	16.0
98	7.0
99	4.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	492178.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.86979607065094
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.66994119937891	47.76631650007152
2	5.667067478551694	6.105675387763956
3	1.6960253706146151	2.7409362253697847
4	0.8776595658574116	1.891173673287792
5	0.5647205485055226	1.5210690392449324
6	0.353813638357804	1.1435921127209967
7	0.26840163791162863	1.012111904952969
8	0.21234875831761887	0.9151347445140857
9	0.1672826864699571	0.8110335785658507
>10	1.2423334742784196	13.357197312148179
>50	0.15628546565336715	5.971829134546598
>100	0.11882236370840292	12.320375805221833
>500	0.0034057365394128225	1.2212742223284028
>1k	0.0015136606841834766	2.1290248669667564
>5k	3.7841517104586915E-4	1.0932554922963151
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5363	1.089646428731069	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3988	0.8102759570724414	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3729	0.7576527191381979	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1440	0.29257707577339903	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	1287	0.2614907614724754	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	861	0.17493670988951152	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	736	0.14953939428418175	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	681	0.13836457541783664	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	672	0.13653596869425288	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	669	0.13592643311972497	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	627	0.12739293507633417	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA	585	0.11885943703294337	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	582	0.11824990145841545	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	578	0.11743718735904489	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	499	0.10138608389647648	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	2.0317852484263822E-4	0.0
11	2.0317852484263822E-4	0.0	0.0	2.0317852484263822E-4	0.0
12	2.0317852484263822E-4	0.0	0.0	4.0635704968527644E-4	0.0
13	2.0317852484263822E-4	0.0	0.0	4.0635704968527644E-4	0.0
14	2.0317852484263822E-4	0.0	0.0	6.095355745279147E-4	0.0
15	2.0317852484263822E-4	0.0	0.0	0.0014222496738984676	0.0
16	2.0317852484263822E-4	0.0	0.0	0.00345403492232485	0.0
17	4.0635704968527644E-4	0.0	0.0	0.0056889986955938705	0.0
18	4.0635704968527644E-4	0.0	0.0	0.0069080698446497	0.0
19	4.0635704968527644E-4	0.0	0.0	0.008736676568233443	0.0
20	4.0635704968527644E-4	0.0	0.0	0.010768461816659827	0.0
21	4.0635704968527644E-4	0.0	0.0	0.011987532965715655	0.0
22	4.0635704968527644E-4	0.0	0.0	0.015644746412883145	0.0
23	4.0635704968527644E-4	0.0	0.0	0.024381422981116587	0.0
24	4.0635704968527644E-4	0.0	0.0	0.03555624184746169	0.0
25	4.0635704968527644E-4	0.0	0.0	0.04043252644368501	0.0
26	4.0635704968527644E-4	0.0	0.0	0.049169203011918454	0.0
27	4.0635704968527644E-4	0.0	0.0	0.0812714099370553	0.0
28	4.0635704968527644E-4	0.0	0.0	0.18590835023101399	0.0
29	4.0635704968527644E-4	0.0	0.0	0.3624704883192666	0.0
30	4.0635704968527644E-4	0.0	0.0	0.6079101463291736	0.0
31	6.095355745279147E-4	0.0	0.0	1.1516158788080735	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACT	60	4.3122782E-8	27.749998	4
TCTAATA	40	0.0019296271	23.125	2
CGCCGAA	40	0.0019296271	23.125	14
TAGATGC	50	2.6990613E-4	22.199999	37
AATACTG	50	2.6990613E-4	22.199999	5
AAGACGG	195	0.0	21.820513	5
AGACGGA	205	0.0	21.658537	6
GTTCTAG	60	3.7207174E-5	21.583332	1
CAAGACG	185	0.0	21.0	4
TTAGACT	45	0.003822747	20.555555	4
GTCTTGG	55	5.137353E-4	20.181818	1
AGTCGGT	775	0.0	19.574192	11
GACGGAC	210	0.0	19.38095	7
GCGCAAG	220	0.0	19.340908	1
CGCAAGA	220	0.0	19.340908	2
GCAGTCG	800	0.0	19.193748	9
GGCAGTC	815	0.0	19.067484	8
AGGACCG	215	0.0	18.930233	5
ACGGACC	215	0.0	18.930233	8
CAGTCGG	815	0.0	18.84049	10
>>END_MODULE