FastQCFastQC Report
Fri 10 Feb 2017
ERR1631264.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631264.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences468151
Sequences flagged as poor quality0
Sequence length43
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT50621.081275058688329No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCAT38220.8164032545054908No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC37160.793760987373732No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGAC36280.7749636335284983No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTC33970.7256205796847598No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTG30400.6493631328353459No Hit
CGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA27060.5780186307409362No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGA26490.5658430720002734No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAG26300.5617845524200524No Hit
CGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCC17990.3842777223588116No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGC17270.3688980692127113No Hit
GCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGAT15520.33151696781593976No Hit
GGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAG15260.325963204179848No Hit
ATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC15070.32190468459962707No Hit
GGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACAT14850.31720534613831863No Hit
GTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCG14670.31336043285179355No Hit
GAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTA13880.2964855356498224No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA12970.2770473629235012No Hit
CTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTC12480.26658065453240515No Hit
ATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCAC12370.26423098530175093No Hit
TGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGA12370.26423098530175093No Hit
GACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGA11910.2544050957917424No Hit
CCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCG11590.24756969439347562No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGC11230.23987986782042547No Hit
ATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGAC11200.23923904893933795No Hit
GGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCG10700.22855873425454606No Hit
GGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTG10390.22193693914997512No Hit
ACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCG10360.2212961202688876No Hit
TCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAAT10300.2200144825067126No Hit
GCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCG10290.21980087621301675No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC10110.2159559629264917No Hit
ACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCC10050.21467432516431664No Hit
GTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTG9950.21253826222735828No Hit
CCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCC9890.21125662446518323No Hit
GGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGT9840.21018859299670403No Hit
CCCGTGGTCACCATGGTAGGCACGGCGACTACCATCGAAAGTT9790.2091205615282249No Hit
GGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGT9450.20185794754256642No Hit
ATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACC9370.2001490971929997No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC8820.18840075103972864No Hit
GTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAAT8820.18840075103972864No Hit
TTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCA8680.1854102629279869No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC8500.18156534964146184No Hit
CCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCA8440.18028371187928682No Hit
GAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAG8410.1796428929981993No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC8380.17900207411711178No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC8180.17472994824319504No Hit
TCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGG8150.17408912936210752No Hit
CCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGAC8100.17302109789362835No Hit
GTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCC8010.1710986412503658No Hit
ACTTAGAACTGGTGCGGACCAGGGGAATCCGACTGTTTAATTA8010.1710986412503658No Hit
CCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCC7910.1689625783134074No Hit
CCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGG7860.16789454684492824No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC7830.16725372796384072No Hit
GGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAA7820.1670401216701449No Hit
GGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC7490.15999111397818225No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA7160.15294210628621963No Hit
CGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCT7150.15272849999252378No Hit
GAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAA7120.15208768111143628No Hit
CCCGTCACCCGTGGTCACCATGGTAGGCACGGCGACTACCATC7100.1516604685240446No Hit
GCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGT6720.1435434293636028No Hit
GCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTG6720.1435434293636028No Hit
GGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAAC6720.1435434293636028No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT6690.14290261048251526No Hit
CCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCT6680.1426890041888194No Hit
GAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCA6640.14183457901403607No Hit
CACCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGC6450.13777605943381516No Hit
AAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGG6390.13649442167164014No Hit
GTGGTCACCATGGTAGGCACGGCGACTACCATCGAAAGTTGAT6380.13628081537794431No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCTGT6300.1345719650283776No Hit
GCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT6230.13307672097250672No Hit
GGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAG6210.13264950838511508No Hit
ACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGT6200.13243590209141923No Hit
GGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGAGGTA6190.13222229579772338No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATCT6100.13029983915446083No Hit
TTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTC6020.12859098880489414No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG6010.12837738251119832No Hit
GCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCG5950.1270957447490233No Hit
GGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGCTGT5930.1266685321616316No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCT5890.12581410698684828No Hit
CGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACG5820.1243188629309774No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5770.12325083146249821No Hit
GGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGT5760.12303722516880237No Hit
CTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCGAC5700.12175558740662736No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG5630.1202603433507565No Hit
GTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTG5600.11961952446966898No Hit
ATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAG5580.11919231188227729No Hit
ATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGCT5570.11897870558858145No Hit
TCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCC5550.11855149300118979No Hit
CCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGG5520.11791067412010227No Hit
CTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACC5470.1168426426516231No Hit
ACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGC5450.11641543006423141No Hit
CATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGT5360.11449297342096887No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC5270.11257051677770634No Hit
TCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCC5200.11107527272183548No Hit
TGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGA5180.1106480601344438No Hit
CTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGT5090.10872560349118125No Hit
ACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCT5020.1072303594353104No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT4940.1055215090857437No Hit
CGTCAACACCCGCCGCGGGCCTTCGCGATGCTTTGTTTTAATT4800.10253102097400198No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC4790.10231741468030614No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC4740.10124938321182696No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTTA251.2325084E-436.9999968
TGTTTCT401.5973856E-632.37534
GACCGTT352.3853068E-531.7142877
TCCTAAG352.3853068E-531.7142872
TAAGGTA352.3853068E-531.7142875
GGACCGT352.3853068E-531.7142876
TAAGTTC303.5962483E-430.83333430
TGTTACG250.00549305829.59999816
TTGTTAC250.00549305829.59999815
TTATTGT250.00549305829.59999812
GTACGAA250.00549305829.5999981
TCGGGTA358.8615116E-426.42857225
ATTGTTA358.8615116E-426.42857214
AAACAGG358.8615116E-426.42857223
GTAAGTT358.8615116E-426.42857229
CCGTTAT358.8615116E-426.4285729
TTGTTTC509.076068E-625.89999833
GGTATCA7450.025.577181
ACTTCGG601.334578E-624.6666688
TGGGAGC451.3215761E-424.6666666
Produced by FastQC (version 0.11.5)