FastQCFastQC Report
Fri 10 Feb 2017
ERR1631263.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631263.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73830
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2430.329134498171475No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1980.26818366517675746No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1810.24515779493430853No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA1790.2424488690234322No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC1710.23161316537992685No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA1670.22619531355817418No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA1130.15305431396451305No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC1130.15305431396451305No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC1080.1462819991873222No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT990.13409183258837873No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA950.12867398076662603No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT950.12867398076662603No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC900.12190166598943519No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG890.12054720303399703No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA870.11783827712312069No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT850.11512935121224435No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC830.112420425301368No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT820.11106596234592984No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT780.10564811052417716No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG770.104293647568739No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC750.10158472165786266No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCACAG358.790662E-426.42857212
GGTATCA2000.021.2750021
GCAGTCG1059.385985E-1021.1428579
AGTCGGT1059.385985E-1021.14285711
ACGCTGA450.003792489420.55555711
TTCCTCG909.1804395E-820.55555720
CTCAGTT450.003792489420.55555716
TCGGTGA1001.2414603E-820.3513
GTCGGTG1001.2414603E-820.3512
GGCAGTC1101.6771082E-920.1818188
GCCTTAT1052.1749656E-819.3809526
CAGTCGG1152.91584E-919.30434810
TCCTCGC1002.7855094E-718.521
ATTCCTC1103.7083737E-818.519
GCACACG500.00697420218.57
GGGCAGT1204.947651E-918.57
CTCTATG1700.018.51
CATCACA500.00697420218.511
CGCCTTA1156.167829E-817.69565225
CGGTGAT1156.167829E-817.69565214
Produced by FastQC (version 0.11.5)