##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631263.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	73830
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.030218068535824	34.0	33.0	34.0	31.0	34.0
2	33.17555194365434	34.0	33.0	34.0	31.0	34.0
3	33.24267912772586	34.0	34.0	34.0	31.0	34.0
4	36.49160232967628	37.0	37.0	37.0	35.0	37.0
5	36.48476229175132	37.0	37.0	37.0	35.0	37.0
6	36.550453745090074	37.0	37.0	37.0	35.0	37.0
7	36.54621427603955	37.0	37.0	37.0	35.0	37.0
8	36.5366382229446	37.0	37.0	37.0	35.0	37.0
9	38.379818501963975	39.0	39.0	39.0	37.0	39.0
10	38.34430448327238	39.0	39.0	39.0	37.0	39.0
11	38.40453745090072	39.0	39.0	39.0	37.0	39.0
12	38.36919951239334	39.0	39.0	39.0	37.0	39.0
13	38.375402952729246	39.0	39.0	39.0	37.0	39.0
14	39.926845455776785	41.0	40.0	41.0	38.0	41.0
15	39.93532439387783	41.0	40.0	41.0	38.0	41.0
16	39.890437491534605	41.0	40.0	41.0	38.0	41.0
17	39.860395503182986	41.0	40.0	41.0	38.0	41.0
18	39.86426926723554	41.0	40.0	41.0	38.0	41.0
19	39.896329405390766	41.0	40.0	41.0	38.0	41.0
20	39.889570635243125	41.0	40.0	41.0	38.0	41.0
21	39.86865772721116	41.0	40.0	41.0	38.0	41.0
22	39.807747528105104	41.0	40.0	41.0	38.0	41.0
23	39.74987132601923	41.0	40.0	41.0	38.0	41.0
24	39.72833536502777	41.0	40.0	41.0	38.0	41.0
25	39.72362183394284	41.0	40.0	41.0	37.0	41.0
26	39.62646620614926	41.0	40.0	41.0	37.0	41.0
27	39.57314099959366	41.0	40.0	41.0	37.0	41.0
28	39.47266693755926	41.0	40.0	41.0	37.0	41.0
29	39.40545848571042	41.0	39.0	41.0	36.0	41.0
30	39.33959095218746	41.0	39.0	41.0	36.0	41.0
31	39.2891913856156	41.0	39.0	41.0	35.0	41.0
32	39.212623594744684	41.0	39.0	41.0	35.0	41.0
33	39.13100365704998	41.0	39.0	41.0	35.0	41.0
34	39.06115400243803	41.0	39.0	41.0	35.0	41.0
35	38.96654476500068	41.0	39.0	41.0	35.0	41.0
36	38.906230529595014	41.0	39.0	41.0	35.0	41.0
37	38.85244480563457	40.0	39.0	41.0	35.0	41.0
38	38.7812948665854	40.0	38.0	41.0	35.0	41.0
39	38.67938507381823	40.0	38.0	41.0	35.0	41.0
40	38.58989570635243	40.0	38.0	41.0	35.0	41.0
41	38.47566030069078	40.0	38.0	41.0	35.0	41.0
42	38.338994988487066	40.0	37.0	41.0	35.0	41.0
43	37.52574834078288	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	0.0
16	0.0
17	2.0
18	0.0
19	1.0
20	4.0
21	5.0
22	15.0
23	29.0
24	56.0
25	60.0
26	95.0
27	146.0
28	192.0
29	260.0
30	354.0
31	526.0
32	614.0
33	848.0
34	1255.0
35	1884.0
36	3007.0
37	6361.0
38	14218.0
39	43897.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.4918054991196	16.177705539753486	12.817282947311389	27.513206013815523
2	20.07178653663822	18.91236624678315	33.29134498171475	27.72450223486388
3	20.204523906271163	20.921034809697954	27.800352160368412	31.074089123662468
4	13.98483001489909	15.623730190979277	33.33468779628877	37.05675199783286
5	16.281999187322228	36.03819585534336	31.55356900988758	16.126235947446837
6	35.29053230394148	32.49627522687255	16.615197074360015	15.59799539482595
7	32.016795340647434	28.61167547067588	19.233373967221997	20.138155221454692
8	30.736827847758363	30.247866720845185	18.220235676554246	20.795069754842206
9	28.501963971285388	13.382093999729108	17.828795882432615	40.28714614655289
10	19.291615874305837	23.837193552756332	29.10469998645537	27.766490586482462
11	37.95069754842205	21.774346471624	18.35568197209806	21.919274007855886
12	24.26791277258567	24.30583773533794	26.333468779628877	25.092780712447514
13	32.36624678315048	17.35067045916294	23.856156034132468	26.42692672355411
14	24.152783421373424	19.30651496681566	22.43261546796695	34.10808614384397
15	28.832452932412302	24.78667208451849	19.795476093728837	26.58539888934038
16	27.629689827983206	23.826357849112828	22.29310578355682	26.25084653934715
17	25.35419206284708	25.25396180414466	23.07598537180008	26.31586076120818
18	26.621969389137206	21.7459027495598	25.018285249898415	26.613842611404575
19	25.882432615467966	24.834078287958825	23.243938778274416	26.039550318298794
20	27.11363944196126	21.88676689692537	24.217797643234455	26.78179601787891
21	29.34308546661249	21.80414465664364	23.503995665718545	25.348774211025326
22	28.906948394961397	21.778409860490317	22.348638764729785	26.966002979818505
23	26.521739130434785	22.131924691859677	23.00148990925098	28.34484626845456
24	25.986726263036708	23.266964648516865	24.11892184748747	26.62738724095896
25	26.240010835703643	22.66287417039144	23.26154679669511	27.83556819720981
26	27.552485439523227	23.032642557226062	23.363131518352972	26.05174048489774
27	27.496952458350265	22.145469321414062	23.01774346471624	27.339834755519437
28	26.112691317892455	22.4095895977245	24.005146959230665	27.47257212515238
29	24.913991602329677	22.931057835568197	25.382635784911283	26.772314777190843
30	23.944196126235948	24.403359068129486	26.031423540566166	25.6210212650684
31	26.08831098469457	23.881890830285794	23.109846945686037	26.919951239333606
32	25.163212786130302	22.69809020723283	25.191656508194498	26.947040498442366
33	25.11851550860084	22.06961939590952	25.593932005959637	27.21793308953
34	26.351076798049572	22.272788839225246	24.395232290396855	26.980902072328323
35	25.630502505756468	22.15224163619125	25.375863470134092	26.84139238791819
36	23.547338480292566	23.005553298117295	26.046322633075984	27.400785588514154
37	25.113097656779086	21.493972639848298	26.475687389949886	26.91724231342273
38	23.3428145740214	20.138155221454692	28.340782879588243	28.17824732493566
39	24.647162400108357	19.890288500609508	28.32046593525667	27.142083164025465
40	23.39563862928349	20.108357036435052	28.488419341731003	28.007584992550456
41	20.904781254232695	21.40593254774482	30.42665583096302	27.262630367059458
42	21.978870377895166	20.853311661926046	30.050115129351212	27.11770283082758
43	20.16930786942977	21.690369768386837	30.189624813761345	27.95069754842205
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4.0
1	3.5
2	3.0
3	11.0
4	19.0
5	19.0
6	33.0
7	47.0
8	42.5
9	38.0
10	55.0
11	72.0
12	72.0
13	152.0
14	232.0
15	341.0
16	450.0
17	426.5
18	403.0
19	403.0
20	434.0
21	465.0
22	343.5
23	222.0
24	248.0
25	274.0
26	274.0
27	329.5
28	385.0
29	484.0
30	583.0
31	691.5
32	800.0
33	800.0
34	1021.5
35	1243.0
36	1544.5
37	1846.0
38	2185.5
39	2525.0
40	2525.0
41	2790.5
42	3056.0
43	3508.0
44	3960.0
45	4519.0
46	5078.0
47	5078.0
48	5877.5
49	6677.0
50	6638.5
51	6600.0
52	6434.0
53	6268.0
54	6268.0
55	5832.0
56	5396.0
57	5236.5
58	5077.0
59	4986.5
60	4896.0
61	4896.0
62	4705.0
63	4514.0
64	4217.0
65	3920.0
66	3557.0
67	3194.0
68	3194.0
69	2659.5
70	2125.0
71	1820.5
72	1516.0
73	1150.0
74	784.0
75	784.0
76	620.0
77	456.0
78	362.0
79	268.0
80	235.5
81	203.0
82	203.0
83	153.0
84	103.0
85	83.0
86	63.0
87	50.5
88	38.0
89	38.0
90	28.0
91	18.0
92	11.0
93	4.0
94	4.0
95	4.0
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	73830.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.08912366246783
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.77879341864717	67.99810375186239
2	4.310786106032906	6.387647297846404
3	1.360146252285192	3.0231613165379927
4	0.5904936014625228	1.749966138426114
5	0.37842778793418647	1.4018691588785046
6	0.28519195612431447	1.267777326290126
7	0.1919561243144424	0.995530272247054
8	0.14442413162705667	0.8560205878369226
9	0.08592321755027423	0.5729378301503454
>10	0.8135283363802559	11.336854937017472
>50	0.04387568555758684	2.415007449546255
>100	0.016453382084095063	1.9951239333604227
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	243	0.329134498171475	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	198	0.26818366517675746	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	181	0.24515779493430853	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	179	0.2424488690234322	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	171	0.23161316537992685	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	167	0.22619531355817418	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	113	0.15305431396451305	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	113	0.15305431396451305	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	108	0.1462819991873222	No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT	99	0.13409183258837873	No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA	95	0.12867398076662603	No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT	95	0.12867398076662603	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	90	0.12190166598943519	No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG	89	0.12054720303399703	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA	87	0.11783827712312069	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	85	0.11512935121224435	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC	83	0.112420425301368	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT	82	0.11106596234592984	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT	78	0.10564811052417716	No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG	77	0.104293647568739	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	75	0.10158472165786266	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0013544629554381688	0.0
3	0.0	0.0	0.0	0.0013544629554381688	0.0
4	0.0	0.0	0.0	0.0013544629554381688	0.0
5	0.0	0.0	0.0	0.0013544629554381688	0.0
6	0.0	0.0	0.0	0.0013544629554381688	0.0
7	0.0	0.0	0.0	0.0013544629554381688	0.0
8	0.0	0.0	0.0	0.0013544629554381688	0.0
9	0.0	0.0	0.0	0.0013544629554381688	0.0
10	0.0	0.0	0.0	0.0013544629554381688	0.0
11	0.0	0.0	0.0	0.0013544629554381688	0.0
12	0.0	0.0	0.0	0.0013544629554381688	0.0
13	0.0	0.0	0.0	0.0013544629554381688	0.0
14	0.0	0.0	0.0	0.0027089259108763375	0.0
15	0.0	0.0	0.0	0.0027089259108763375	0.0
16	0.0	0.0	0.0	0.009481240688067182	0.0
17	0.0	0.0	0.0	0.012190166598943519	0.0
18	0.0	0.0	0.0	0.013544629554381687	0.0
19	0.0	0.0	0.0	0.016253555465258026	0.0
20	0.0	0.0	0.0	0.017608018420696193	0.0
21	0.0	0.0	0.0	0.017608018420696193	0.0
22	0.0	0.0	0.0	0.0216714072870107	0.0
23	0.0	0.0	0.0	0.024380333197887037	0.0
24	0.0	0.0	0.0	0.02979818501963971	0.0
25	0.0	0.0	0.0	0.037924962752268726	0.0
26	0.0	0.0	0.0	0.058241907083841256	0.0
27	0.0	0.0	0.0	0.10700257347961534	0.0
28	0.0	0.0	0.0	0.3331978870377895	0.0
29	0.0	0.0	0.0	0.6799404036299607	0.0
30	0.0	0.0	0.0	1.1648381416768252	0.0
31	0.0	0.0	0.0	2.18203982121089	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCACAG	35	8.790662E-4	26.428572	12
GGTATCA	200	0.0	21.275002	1
GCAGTCG	105	9.385985E-10	21.142857	9
AGTCGGT	105	9.385985E-10	21.142857	11
ACGCTGA	45	0.0037924894	20.555557	11
TTCCTCG	90	9.1804395E-8	20.555557	20
CTCAGTT	45	0.0037924894	20.555557	16
TCGGTGA	100	1.2414603E-8	20.35	13
GTCGGTG	100	1.2414603E-8	20.35	12
GGCAGTC	110	1.6771082E-9	20.181818	8
GCCTTAT	105	2.1749656E-8	19.38095	26
CAGTCGG	115	2.91584E-9	19.304348	10
TCCTCGC	100	2.7855094E-7	18.5	21
ATTCCTC	110	3.7083737E-8	18.5	19
GCACACG	50	0.006974202	18.5	7
GGGCAGT	120	4.947651E-9	18.5	7
CTCTATG	170	0.0	18.5	1
CATCACA	50	0.006974202	18.5	11
CGCCTTA	115	6.167829E-8	17.695652	25
CGGTGAT	115	6.167829E-8	17.695652	14
>>END_MODULE