FastQCFastQC Report
Fri 10 Feb 2017
ERR1631262.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631262.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104639
Sequences flagged as poor quality0
Sequence length43
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT34303.277936524622751No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT33023.1556111965901814No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT22512.1512055734477586No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13561.2958839438450291No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7280.6957253031852368No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA7250.6928583033094735No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA4540.4338726478655186No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4380.4185819818614474No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3900.37270998384923404No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3750.3583749844704174No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA2790.2666309884459905No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA2770.26471965519548163No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2460.23509398981259375No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA2440.23318265656208487No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG2340.2236259903095404No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA2320.2217146570590315No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA2270.2169363239327593No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2180.20833532430546928No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2120.2026013245539426No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA2010.1920889916761437No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1990.1901776584256348No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT1960.18731065854987147No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1900.18157665879834478No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC1880.1796653255478359No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT1870.17870965892258145No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC1840.17584265904681812No Hit
GCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA1830.17488699242156366No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT1720.16437465954376476No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT1640.1567293265417292No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC1580.1509953267902025No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT1550.14812832691443917No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT1540.14717266028918471No Hit
GAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAA1530.14621699366393026No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA1460.13952732728714914No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC1460.13952732728714914No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT1450.13857166066189472No Hit
GAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1390.13283766091036803No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT1380.13188199428511357No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT1240.11850266153155134No Hit
GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAA1230.11754699490629687No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1230.11754699490629687No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCT1160.11085732852951576No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGAGGCCC1160.11085732852951576No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC1150.10990166190426132No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACCTGTCT1140.10894599527900688No Hit
ATCACCGACTGCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAA1110.10607899540324355ABI Solid3 Adapter B (95% over 23bp)
TGCCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCT1110.10607899540324355No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCT1100.10512332877798908No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGA1090.10416766215273464ABI Solid3 Adapter B (100% over 21bp)
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG1080.1032119955274802No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATA200.001834298137.02
TATACCA200.001834298137.05
ATGCCCC403.7765858E-837.037
GCGTTAT200.001834298137.01
CTTTGGG200.001834298137.036
TTGCCCT303.57735E-430.8333324
TTTTGCC250.005473815429.5999982
TTATACC250.005473815429.5999984
TGCCCTC250.005473815429.5999985
GTTATAC250.005473815429.5999983
GGAAGAG400.001919538123.12500220
ATGCTTC502.6794185E-422.19999911
CATGCTT502.6794185E-422.19999910
CCATGCT603.6847785E-521.5833329
ACCATGC701.2058081E-418.58
ATACCAT902.1144297E-618.56
TACCATG801.5939033E-518.57
AGCTGAT752.0447258E-417.2666667
CTTATAC2900.017.22413837
GACTGTC956.962131E-515.57894834
Produced by FastQC (version 0.11.5)