##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631262.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	104639
Sequences flagged as poor quality	0
Sequence length	43
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.91636005695773	34.0	33.0	34.0	31.0	34.0
2	32.98287445407544	34.0	33.0	34.0	31.0	34.0
3	32.963464864916524	34.0	33.0	34.0	31.0	34.0
4	36.28401456435937	37.0	37.0	37.0	35.0	37.0
5	36.31446210303998	37.0	37.0	37.0	35.0	37.0
6	36.448981737210794	37.0	37.0	37.0	35.0	37.0
7	36.454209233650936	37.0	37.0	37.0	35.0	37.0
8	36.5130400711016	37.0	37.0	37.0	35.0	37.0
9	38.272584791521325	39.0	39.0	39.0	37.0	39.0
10	38.26696547176483	39.0	39.0	39.0	37.0	39.0
11	38.337417215378586	39.0	39.0	39.0	37.0	39.0
12	38.358317644472905	39.0	39.0	39.0	37.0	39.0
13	38.346085111669645	39.0	39.0	39.0	37.0	39.0
14	39.73285295157637	41.0	40.0	41.0	37.0	41.0
15	39.68164833379524	41.0	40.0	41.0	37.0	41.0
16	39.5395407066199	41.0	39.0	41.0	37.0	41.0
17	39.627624499469604	41.0	39.0	41.0	37.0	41.0
18	39.747130610957676	41.0	40.0	41.0	38.0	41.0
19	39.78179263945565	41.0	40.0	41.0	38.0	41.0
20	39.76739074341307	41.0	40.0	41.0	38.0	41.0
21	39.71601410563939	41.0	40.0	41.0	37.0	41.0
22	39.579611808216825	41.0	40.0	41.0	36.0	41.0
23	39.46318294326207	41.0	40.0	41.0	35.0	41.0
24	39.39208134634314	41.0	39.0	41.0	35.0	41.0
25	39.361519127667506	41.0	39.0	41.0	35.0	41.0
26	39.06814858704689	41.0	38.0	41.0	35.0	41.0
27	38.86699987576334	41.0	38.0	41.0	35.0	41.0
28	38.68821376351074	40.0	37.0	41.0	35.0	41.0
29	38.45243169372796	40.0	37.0	41.0	35.0	41.0
30	38.14494595705234	40.0	36.0	41.0	35.0	41.0
31	37.77064000993893	40.0	35.0	41.0	35.0	41.0
32	37.43105343132102	40.0	35.0	41.0	35.0	41.0
33	37.10726402201856	39.0	35.0	41.0	34.0	41.0
34	36.82280985101157	39.0	35.0	41.0	33.0	41.0
35	36.50581523141467	39.0	35.0	41.0	33.0	41.0
36	36.17223979586961	39.0	35.0	41.0	33.0	41.0
37	35.81307160810023	39.0	35.0	41.0	31.0	41.0
38	35.43013599136077	38.0	35.0	41.0	27.0	41.0
39	34.996444920154055	38.0	35.0	41.0	22.0	41.0
40	34.5291908370684	38.0	35.0	41.0	18.0	41.0
41	33.99188639035159	37.0	35.0	41.0	12.0	41.0
42	33.436529401083725	37.0	35.0	41.0	10.0	41.0
43	32.45871998012213	35.0	33.0	40.0	8.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	1.0
13	0.0
14	0.0
15	2.0
16	1.0
17	6.0
18	4.0
19	7.0
20	9.0
21	40.0
22	56.0
23	137.0
24	184.0
25	286.0
26	443.0
27	675.0
28	1126.0
29	1458.0
30	1898.0
31	2108.0
32	2306.0
33	2830.0
34	3651.0
35	4571.0
36	6632.0
37	18823.0
38	17702.0
39	39681.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.666156977799865	17.11598925830713	16.228174963445753	19.98967880044725
2	18.449144200537084	23.714867305689083	33.48846988216631	24.347518611607526
3	22.184845038656714	25.67685088733646	29.55876871912002	22.5795353548868
4	14.119018721509189	20.033639465208957	29.98404036735825	35.863301445923604
5	12.111163141849596	41.8486415198922	28.974856411089554	17.065338927168646
6	26.282743527747783	30.20384369116677	21.713701392406275	21.799711388679174
7	27.173424822484925	35.186689475243455	19.694377813243626	17.945507889027994
8	34.09818518907864	29.372413727195408	18.66321352459408	17.86618755913187
9	30.998958323378474	13.20349009451543	20.67011343762842	35.12743814447768
10	22.28519003430843	29.498561721728993	24.02832595877254	24.187922285190034
11	29.01212740947448	27.32346448264987	17.21633425395885	26.4480738539168
12	22.298569367061994	34.12207685471	25.68162922046273	17.89772455776527
13	32.35696059786504	20.168388459369833	29.23957606628504	18.23507487648009
14	22.490658358738138	24.0082569596422	30.751440667437567	22.749644014182095
15	30.418868681849027	25.159835243073807	25.2878945708579	19.133401504219268
16	19.8816884717935	26.78542417263162	32.18207360544348	21.1508137501314
17	16.243465629449823	33.21037089421726	30.387331683215628	20.158831793117287
18	18.340198205258076	24.21754795057292	35.393113466298416	22.049140377870582
19	18.29528187387112	30.646317338659586	37.33024971568918	13.72815107178012
20	20.697827769760796	24.38192261011669	38.68442932367473	16.235820296447788
21	23.83050296734487	23.518955647511923	36.19587343151215	16.454667953631056
22	22.083544376379745	24.57018893529181	33.33460755550034	20.011659132828104
23	18.669903190970864	26.733818174867878	34.31989984613767	20.276378788023585
24	17.212511587457833	27.753514464014373	37.357964047821554	17.676009900706237
25	16.633377612553637	27.529888473704833	35.25454180563653	20.58219210810501
26	20.340408451915636	26.01515687267654	35.17044314261413	18.4739915327937
27	18.71004118923155	26.61818251321209	33.729297871730424	20.942478425825936
28	16.686894943567886	27.56715947208976	37.00627872972792	18.739666854614438
29	16.444155620753257	28.38521010330756	37.43824004434293	17.73239423159625
30	15.50377966150288	29.25964506541538	39.60951461692103	15.627060656160705
31	16.263534628580167	30.92537199323388	35.55557679259167	17.25551658559428
32	15.121513011401102	30.576553675016005	38.4837393323713	15.818193981211595
33	16.52443161727463	28.804747751794263	37.76603369680521	16.904786934125898
34	16.854136602987413	29.79481837555787	33.810529534877055	19.54051548657766
35	16.122095968042508	30.854652662965048	33.8219975343801	19.201253834612334
36	13.974713061095766	30.752396334062826	35.34724146828621	19.925649136555204
37	15.03263601525244	30.338592685327647	35.35488680128824	19.27388449813167
38	15.36138533433997	27.508863807949236	37.09611139250184	20.033639465208957
39	16.58368294804041	27.617809803228244	37.12955972438575	18.668947524345608
40	15.614636990032396	27.498351475071438	35.18382247536769	21.703189059528473
41	13.035292768470647	28.3928554363096	36.997677730100634	21.574174065119124
42	13.107923431989985	27.940825122564245	35.77251311652443	23.178738328921337
43	12.277449134643872	30.48003134586531	36.72435707527786	20.51816244421296
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	15.0
1	38.0
2	61.0
3	238.0
4	415.0
5	415.0
6	566.0
7	717.0
8	813.0
9	909.0
10	1366.0
11	1823.0
12	1823.0
13	3227.0
14	4631.0
15	6110.5
16	7590.0
17	6608.5
18	5627.0
19	5627.0
20	6273.5
21	6920.0
22	4783.0
23	2646.0
24	2229.0
25	1812.0
26	1812.0
27	1690.5
28	1569.0
29	1673.0
30	1777.0
31	1821.0
32	1865.0
33	1865.0
34	1724.5
35	1584.0
36	1539.5
37	1495.0
38	1735.0
39	1975.0
40	1975.0
41	2391.0
42	2807.0
43	3598.5
44	4390.0
45	5572.5
46	6755.0
47	6755.0
48	9020.5
49	11286.0
50	10533.5
51	9781.0
52	7363.5
53	4946.0
54	4946.0
55	4037.5
56	3129.0
57	2978.5
58	2828.0
59	2779.5
60	2731.0
61	2731.0
62	2625.5
63	2520.0
64	2467.5
65	2415.0
66	2283.5
67	2152.0
68	2152.0
69	1979.5
70	1807.0
71	1628.5
72	1450.0
73	1207.5
74	965.0
75	965.0
76	785.0
77	605.0
78	475.0
79	345.0
80	256.0
81	167.0
82	167.0
83	127.0
84	87.0
85	56.5
86	26.0
87	17.5
88	9.0
89	9.0
90	7.5
91	6.0
92	3.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	104639.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.57371534513901
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.30874614245705	47.310276283221356
2	5.776057368130006	6.188897065147794
3	1.8926488164255517	3.0418868681849025
4	0.9704061792040528	2.0795305765536747
5	0.6047200271142903	1.6198549298062863
6	0.43168804295474417	1.387627939869456
7	0.28184591234235357	1.0569672875314176
8	0.2015733423714301	0.8639226292300194
9	0.14984213061239052	0.7224839686923614
>10	1.1898178704579103	12.381616796796607
>50	0.09454324907686544	3.5608138456980667
>100	0.08740790952389448	8.516900964267625
>500	0.003567669776485488	1.3885836064947104
>1k	0.007135339552970976	9.88063723850572
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3430	3.277936524622751	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3302	3.1556111965901814	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2251	2.1512055734477586	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1356	1.2958839438450291	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	728	0.6957253031852368	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	725	0.6928583033094735	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	454	0.4338726478655186	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	438	0.4185819818614474	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	390	0.37270998384923404	No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	375	0.3583749844704174	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA	279	0.2666309884459905	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA	277	0.26471965519548163	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	246	0.23509398981259375	No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA	244	0.23318265656208487	No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG	234	0.2236259903095404	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA	232	0.2217146570590315	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	227	0.2169363239327593	No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	218	0.20833532430546928	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	212	0.2026013245539426	No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA	201	0.1920889916761437	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	199	0.1901776584256348	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT	196	0.18731065854987147	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	190	0.18157665879834478	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC	188	0.1796653255478359	No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT	187	0.17870965892258145	No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC	184	0.17584265904681812	No Hit
GCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA	183	0.17488699242156366	No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT	172	0.16437465954376476	No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT	164	0.1567293265417292	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC	158	0.1509953267902025	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT	155	0.14812832691443917	No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT	154	0.14717266028918471	No Hit
GAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAA	153	0.14621699366393026	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	146	0.13952732728714914	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC	146	0.13952732728714914	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	145	0.13857166066189472	No Hit
GAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	139	0.13283766091036803	No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT	138	0.13188199428511357	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT	124	0.11850266153155134	No Hit
GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAA	123	0.11754699490629687	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	123	0.11754699490629687	No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCT	116	0.11085732852951576	No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGAGGCCC	116	0.11085732852951576	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC	115	0.10990166190426132	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACCTGTCT	114	0.10894599527900688	No Hit
ATCACCGACTGCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAA	111	0.10607899540324355	ABI Solid3 Adapter B (95% over 23bp)
TGCCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCT	111	0.10607899540324355	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCT	110	0.10512332877798908	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGA	109	0.10416766215273464	ABI Solid3 Adapter B (100% over 21bp)
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG	108	0.1032119955274802	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	9.556666252544463E-4	0.0
4	0.0	0.0	0.0	0.0019113332505088926	0.0
5	0.0	0.0	0.0	0.0019113332505088926	0.0
6	0.0	0.0	0.0	0.0019113332505088926	0.0
7	0.0	0.0	0.0	0.0019113332505088926	0.0
8	0.0	0.0	0.0	0.0019113332505088926	0.0
9	0.0	0.0	0.0	0.0019113332505088926	0.0
10	0.0	0.0	0.0	0.0047783331262722314	0.0
11	0.0	0.0	0.0	0.008600999627290017	0.0
12	0.0	0.0	0.0	0.012423666128307802	0.0
13	0.0	0.0	0.0	0.012423666128307802	0.0
14	0.0	0.0	0.0	0.016246332629325587	0.0
15	0.0	0.0	0.0	0.020068999130343372	0.0
16	0.0	0.0	0.0	0.040137998260686744	0.0
17	0.0	0.0	0.0	0.05638433089001233	0.0
18	0.0	0.0	0.0	0.06785233039306568	0.0
19	0.0	0.0	0.0	0.07263066351933792	0.0
20	0.0	0.0	0.0	0.08314299639713682	0.0
21	0.0	0.0	0.0	0.1013006622769713	0.0
22	0.0	0.0	0.0	0.13379332753562248	0.0
23	0.0	0.0	0.0	0.17584265904681812	0.0
24	0.0	0.0	0.0	0.2580299888187005	0.0
25	0.0	0.0	0.0	0.28669998757633386	0.0
26	0.0	0.0	0.0	0.3239709859612573	0.0
27	0.0	0.0	0.0	0.42718298148873746	0.0
28	0.0	0.0	0.0	0.6909469700589647	0.0
29	0.0	0.0	0.0	1.120041284798211	0.0
30	0.0	0.0	0.0	1.7201999254580032	0.0
31	0.0	0.0	0.0	2.863177209262321	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTATA	20	0.0018342981	37.0	2
TATACCA	20	0.0018342981	37.0	5
ATGCCCC	40	3.7765858E-8	37.0	37
GCGTTAT	20	0.0018342981	37.0	1
CTTTGGG	20	0.0018342981	37.0	36
TTGCCCT	30	3.57735E-4	30.833332	4
TTTTGCC	25	0.0054738154	29.599998	2
TTATACC	25	0.0054738154	29.599998	4
TGCCCTC	25	0.0054738154	29.599998	5
GTTATAC	25	0.0054738154	29.599998	3
GGAAGAG	40	0.0019195381	23.125002	20
ATGCTTC	50	2.6794185E-4	22.199999	11
CATGCTT	50	2.6794185E-4	22.199999	10
CCATGCT	60	3.6847785E-5	21.583332	9
ACCATGC	70	1.2058081E-4	18.5	8
ATACCAT	90	2.1144297E-6	18.5	6
TACCATG	80	1.5939033E-5	18.5	7
AGCTGAT	75	2.0447258E-4	17.266666	7
CTTATAC	290	0.0	17.224138	37
GACTGTC	95	6.962131E-5	15.578948	34
>>END_MODULE