FastQCFastQC Report
Fri 10 Feb 2017
ERR1631261.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631261.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2904
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT160.5509641873278237No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT130.4476584022038568No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT90.30991735537190085No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC80.27548209366391185No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT70.24104683195592286TruSeq Adapter, Index 3 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGT70.24104683195592286No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG60.2066115702479339No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA60.2066115702479339No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT50.17217630853994492No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGAC50.17217630853994492No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.17217630853994492No Hit
GCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGC50.17217630853994492No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC50.17217630853994492No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCCTGTCTCTT50.17217630853994492No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC40.13774104683195593No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC40.13774104683195593No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT40.13774104683195593No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC40.13774104683195593No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA40.13774104683195593No Hit
ATCATATTCATCGGCGTAAATCTAACTTTCTTCCCACAACACT40.13774104683195593No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC40.13774104683195593No Hit
TAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGC40.13774104683195593No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCCTGTCTCTT30.10330578512396695No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG30.10330578512396695No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAAT30.10330578512396695No Hit
GGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC30.10330578512396695No Hit
CTTCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTT30.10330578512396695No Hit
CCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAA30.10330578512396695No Hit
GTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGA30.10330578512396695No Hit
GACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTC30.10330578512396695No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT30.10330578512396695No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC30.10330578512396695No Hit
ACCCAATACCAAACGCCCCTCTTCGTCTGATCCGTCCTAATCC30.10330578512396695No Hit
GTCGGTGATTCCTCCGCTTATTGATATGCCCATAGAGCTGTCT30.10330578512396695No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCCTGTCTCTTATACA30.10330578512396695No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.10330578512396695No Hit
CGTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAAC30.10330578512396695No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC30.10330578512396695No Hit
ATCCGGAATGCCCCGACGTTACTCGGACTACCCCGATGCATAC30.10330578512396695No Hit
GCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGC30.10330578512396695No Hit
GCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCG30.10330578512396695No Hit
CTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTT30.10330578512396695No Hit
CGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACG30.10330578512396695No Hit
CCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGTTAGTC30.10330578512396695No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers