##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631261.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2904
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.95144628099174	34.0	33.0	34.0	31.0	34.0
2	33.09676308539945	34.0	33.0	34.0	31.0	34.0
3	33.205922865013775	34.0	33.0	34.0	31.0	34.0
4	36.446280991735534	37.0	37.0	37.0	35.0	37.0
5	36.455234159779614	37.0	37.0	37.0	35.0	37.0
6	36.474173553719005	37.0	37.0	37.0	35.0	37.0
7	36.481404958677686	37.0	37.0	37.0	35.0	37.0
8	36.49758953168044	37.0	37.0	37.0	35.0	37.0
9	38.34951790633609	39.0	39.0	39.0	37.0	39.0
10	38.28305785123967	39.0	39.0	39.0	37.0	39.0
11	38.325413223140494	39.0	39.0	39.0	37.0	39.0
12	38.28305785123967	39.0	39.0	39.0	37.0	39.0
13	38.309573002754824	39.0	39.0	39.0	37.0	39.0
14	39.86432506887052	41.0	40.0	41.0	38.0	41.0
15	39.850895316804404	41.0	40.0	41.0	38.0	41.0
16	39.809573002754824	41.0	40.0	41.0	38.0	41.0
17	39.79752066115702	41.0	40.0	41.0	38.0	41.0
18	39.765151515151516	41.0	40.0	41.0	38.0	41.0
19	39.839187327823694	41.0	40.0	41.0	38.0	41.0
20	39.79855371900826	41.0	40.0	41.0	38.0	41.0
21	39.80922865013774	41.0	40.0	41.0	38.0	41.0
22	39.76170798898072	41.0	40.0	41.0	37.0	41.0
23	39.68388429752066	41.0	40.0	41.0	37.0	41.0
24	39.607438016528924	41.0	40.0	41.0	37.0	41.0
25	39.48863636363637	41.0	39.0	41.0	37.0	41.0
26	39.45144628099174	41.0	39.0	41.0	36.0	41.0
27	39.39325068870524	41.0	39.0	41.0	36.0	41.0
28	39.294765840220386	41.0	39.0	41.0	35.0	41.0
29	39.27203856749311	41.0	39.0	41.0	35.0	41.0
30	39.12982093663912	41.0	39.0	41.0	35.0	41.0
31	39.02376033057851	40.0	39.0	41.0	35.0	41.0
32	38.960055096418735	40.0	39.0	41.0	35.0	41.0
33	38.83161157024794	40.0	38.0	41.0	35.0	41.0
34	38.7534435261708	40.0	38.0	41.0	35.0	41.0
35	38.71349862258953	40.0	38.0	41.0	35.0	41.0
36	38.6318870523416	40.0	38.0	41.0	35.0	41.0
37	38.539944903581265	40.0	38.0	41.0	35.0	41.0
38	38.442837465564736	40.0	38.0	41.0	35.0	41.0
39	38.35537190082645	40.0	38.0	41.0	35.0	41.0
40	38.162534435261705	40.0	37.0	41.0	34.0	41.0
41	38.078512396694215	40.0	37.0	41.0	34.0	41.0
42	37.97796143250689	40.0	37.0	41.0	34.0	41.0
43	37.097451790633606	39.0	35.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	0.0
21	0.0
22	1.0
23	1.0
24	1.0
25	2.0
26	6.0
27	5.0
28	11.0
29	19.0
30	19.0
31	22.0
32	25.0
33	39.0
34	47.0
35	98.0
36	156.0
37	264.0
38	594.0
39	1593.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.38980716253444	19.765840220385673	11.19146005509642	26.65289256198347
2	20.90220385674931	19.214876033057852	33.16115702479338	26.72176308539945
3	21.763085399449036	22.210743801652892	27.72038567493113	28.305785123966942
4	13.46418732782369	18.009641873278238	31.508264462809915	37.01790633608815
5	15.048209366391186	35.192837465564736	32.85123966942149	16.90771349862259
6	31.92148760330579	35.158402203856745	16.219008264462808	16.701101928374655
7	28.09917355371901	30.81955922865014	20.075757575757574	21.005509641873278
8	28.994490358126722	32.8168044077135	17.83746556473829	20.351239669421485
9	25.413223140495866	14.703856749311296	18.181818181818183	41.701101928374655
10	21.03994490358127	24.311294765840223	27.61707988980716	27.03168044077135
11	35.74380165289256	23.140495867768596	17.52754820936639	23.588154269972453
12	23.829201101928373	28.09917355371901	22.83057851239669	25.24104683195592
13	31.54269972451791	18.49173553719008	23.519283746556475	26.446280991735538
14	23.62258953168044	20.90220385674931	22.176308539944902	33.298898071625345
15	29.786501377410467	25.034435261707987	19.52479338842975	25.654269972451793
16	27.03168044077135	24.483471074380166	22.417355371900825	26.06749311294766
17	25.344352617079892	26.584022038567497	22.486225895316807	25.585399449035812
18	25.37878787878788	22.761707988980717	24.414600550964188	27.444903581267216
19	25.585399449035812	24.89669421487603	23.588154269972453	25.929752066115704
20	25.172176308539946	23.725895316804408	23.898071625344354	27.203856749311296
21	26.377410468319557	25.654269972451793	21.797520661157023	26.170798898071624
22	27.926997245179063	23.002754820936637	23.037190082644628	26.033057851239672
23	25.96418732782369	23.62258953168044	24.242424242424242	26.170798898071624
24	25.344352617079892	25.68870523415978	22.417355371900825	26.549586776859503
25	25.585399449035812	23.34710743801653	24.449035812672175	26.618457300275484
26	25.413223140495866	26.377410468319557	23.106060606060606	25.10330578512397
27	25.895316804407713	23.484848484848484	25.792011019283745	24.827823691460054
28	24.827823691460054	24.276859504132233	24.89669421487603	25.998622589531678
29	23.484848484848484	23.34710743801653	27.066115702479337	26.101928374655646
30	25.10330578512397	25.550964187327825	25.068870523415974	24.276859504132233
31	25.27548209366391	24.6900826446281	23.62258953168044	26.41184573002755
32	23.209366391184574	23.174931129476583	27.548209366391184	26.06749311294766
33	23.037190082644628	22.865013774104685	25.37878787878788	28.719008264462808
34	25.24104683195592	22.69283746556474	25.550964187327825	26.515151515151516
35	23.381542699724516	22.348484848484848	25.826446280991732	28.4435261707989
36	22.141873278236915	23.24380165289256	28.030303030303028	26.584022038567497
37	23.794765840220386	21.143250688705233	28.06473829201102	26.997245179063363
38	20.9366391184573	20.316804407713498	31.16391184573003	27.582644628099175
39	21.935261707988982	20.385674931129476	31.54269972451791	26.136363636363637
40	19.800275482093664	21.935261707988982	30.303030303030305	27.96143250688705
41	19.352617079889807	19.765840220385673	33.574380165289256	27.307162534435264
42	21.62534435261708	21.62534435261708	30.33746556473829	26.41184573002755
43	18.112947658402202	20.695592286501377	33.1267217630854	28.06473829201102
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	0.5
4	0.0
5	0.0
6	1.5
7	3.0
8	2.5
9	2.0
10	4.5
11	7.0
12	7.0
13	12.0
14	17.0
15	22.0
16	27.0
17	23.5
18	20.0
19	20.0
20	24.5
21	29.0
22	19.0
23	9.0
24	10.0
25	11.0
26	11.0
27	11.5
28	12.0
29	22.0
30	32.0
31	36.0
32	40.0
33	40.0
34	50.0
35	60.0
36	73.5
37	87.0
38	110.5
39	134.0
40	134.0
41	143.0
42	152.0
43	181.5
44	211.0
45	210.0
46	209.0
47	209.0
48	218.5
49	228.0
50	227.0
51	226.0
52	226.0
53	226.0
54	226.0
55	219.0
56	212.0
57	193.0
58	174.0
59	181.5
60	189.0
61	189.0
62	170.0
63	151.0
64	137.5
65	124.0
66	111.0
67	98.0
68	98.0
69	84.0
70	70.0
71	64.0
72	58.0
73	43.5
74	29.0
75	29.0
76	23.0
77	17.0
78	17.5
79	18.0
80	12.5
81	7.0
82	7.0
83	6.5
84	6.0
85	4.5
86	3.0
87	3.0
88	3.0
89	3.0
90	2.5
91	2.0
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2904.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	90.08264462809917
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.27217125382263	84.02203856749311
2	5.045871559633028	9.090909090909092
3	0.8409785932721712	2.272727272727273
4	0.3058103975535168	1.1019283746556474
5	0.22935779816513763	1.0330578512396695
6	0.0764525993883792	0.4132231404958678
7	0.0764525993883792	0.4820936639118457
8	0.0382262996941896	0.27548209366391185
9	0.0382262996941896	0.30991735537190085
>10	0.0764525993883792	0.9986225895316805
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	16	0.5509641873278237	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	13	0.4476584022038568	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	9	0.30991735537190085	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	8	0.27548209366391185	No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT	7	0.24104683195592286	TruSeq Adapter, Index 3 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGT	7	0.24104683195592286	No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG	6	0.2066115702479339	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	6	0.2066115702479339	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	5	0.17217630853994492	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGAC	5	0.17217630853994492	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5	0.17217630853994492	No Hit
GCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGC	5	0.17217630853994492	No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC	5	0.17217630853994492	No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCCTGTCTCTT	5	0.17217630853994492	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	4	0.13774104683195593	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	4	0.13774104683195593	No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT	4	0.13774104683195593	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	4	0.13774104683195593	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	4	0.13774104683195593	No Hit
ATCATATTCATCGGCGTAAATCTAACTTTCTTCCCACAACACT	4	0.13774104683195593	No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC	4	0.13774104683195593	No Hit
TAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGC	4	0.13774104683195593	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCCTGTCTCTT	3	0.10330578512396695	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG	3	0.10330578512396695	No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAAT	3	0.10330578512396695	No Hit
GGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC	3	0.10330578512396695	No Hit
CTTCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTT	3	0.10330578512396695	No Hit
CCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAA	3	0.10330578512396695	No Hit
GTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGA	3	0.10330578512396695	No Hit
GACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTC	3	0.10330578512396695	No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT	3	0.10330578512396695	No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC	3	0.10330578512396695	No Hit
ACCCAATACCAAACGCCCCTCTTCGTCTGATCCGTCCTAATCC	3	0.10330578512396695	No Hit
GTCGGTGATTCCTCCGCTTATTGATATGCCCATAGAGCTGTCT	3	0.10330578512396695	No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCCTGTCTCTTATACA	3	0.10330578512396695	No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3	0.10330578512396695	No Hit
CGTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAAC	3	0.10330578512396695	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	3	0.10330578512396695	No Hit
ATCCGGAATGCCCCGACGTTACTCGGACTACCCCGATGCATAC	3	0.10330578512396695	No Hit
GCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGC	3	0.10330578512396695	No Hit
GCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCG	3	0.10330578512396695	No Hit
CTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTT	3	0.10330578512396695	No Hit
CGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACG	3	0.10330578512396695	No Hit
CCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGTTAGTC	3	0.10330578512396695	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.10330578512396695	0.0
27	0.0	0.0	0.0	0.2066115702479339	0.0
28	0.0	0.0	0.0	0.7920110192837465	0.0
29	0.0	0.0	0.0	1.859504132231405	0.0
30	0.0	0.0	0.0	2.479338842975207	0.0
31	0.0	0.0	0.0	4.40771349862259	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE