FastQCFastQC Report
Fri 10 Feb 2017
ERR1631260.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631260.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89564
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA4410.49238533339288104No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC3630.40529677102407213No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC2940.3282568889285874No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC2660.2969943280782457No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC2660.2969943280782457No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG2200.24563440668125586No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC2170.24228484659014785No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCT2030.22665356616497703No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG2010.2244205261042383No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC2000.22330400607386897No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1810.2020901254968514No Hit
TAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGC1780.1987405654057434No Hit
GAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGG1750.19539100531463532No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC1560.1741771247376178No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1520.16971104461614042No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG1480.16524496449466303No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT1430.1596623643428163No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG1380.15407976419096958No Hit
GCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGC1340.14961368406949221No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTCTGTC1190.13286588361395205No Hit
GTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACAC1170.13063284355321333No Hit
GTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGA1150.12839980349247465No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC1130.12616676343173597No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1120.12505024340136664No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA1100.12281720334062793No Hit
CACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGG1090.12170068331025859No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGCTGTC1080.12058416327988923No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC1070.11946764324951989No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA1050.11723460318878122No Hit
GCGTGTGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGA990.11053548300656514No Hit
AGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGG960.1071859229154571No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG940.1049528828547184No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG940.1049528828547184No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC940.1049528828547184No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAG930.10383636282434908No Hit
GAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGC930.10383636282434908No Hit
ACTTAGAACTGGTGCGGACCAGGGGAATCCGACTGTTTAATTA930.10383636282434908No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGT920.10271984279397973No Hit
GCGTAGGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT920.10271984279397973No Hit
GACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTC910.10160332276361037No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT900.10048680273324104No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT900.10048680273324104No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCAT200.001832888637.010
TTCCGAA200.001832888637.020
AAATAGA250.005469654729.635
CGAAAAC250.005469654729.623
GCGGTGT250.005469654729.633
ATGGCCT250.005469654729.610
TCCGAAA250.005469654729.621
AAGACCC250.005469654729.65
ATAGAAC250.005469654729.637
GTGTGTA250.005469654729.636
GACCATA250.005469654729.611
AACGATG250.005469654729.618
TCACTAA250.005469654729.61
CCGACCA250.005469654729.69
ACCAACA250.005469654729.628
ATAAACG250.005469654729.615
AAAATAG250.005469654729.634
GTAGTTC250.005469654729.63
TCAGTTC250.005469654729.616
ACGCCGC358.805422E-426.42857214
Produced by FastQC (version 0.11.5)