FastQCFastQC Report
Fri 10 Feb 2017
ERR1631259.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631259.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1840639
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT47090.25583506597437083No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT36420.19786606716471833No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT33440.18167603750653985No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATATAT855.1850293E-821.7647061
GGTATCA19900.020.6381911
ATAACGT656.905012E-519.92307717
AGTCGGT4400.018.92045411
GCAGTCG4600.018.0978249
CTTATAC27150.017.6482537
CGCCTTA3250.017.64615425
ATACACA6000.017.57537
TCGGTGA5100.017.41176413
TAACGTT752.068118E-417.26666618
TATACTG1301.3946192E-817.0769235
GTGATTC5000.017.0216
CTCTATG7550.016.9072861
CGGTGAT5150.016.88349314
CAGTCGG5200.016.72115310
GTATTAT1801.0913936E-1116.4444451
GTTCTAA803.3839484E-416.18751
GTACTAT2200.015.9772731
ATTAGAC700.002593288715.8571423
GGCAGTC5350.015.5607478
Produced by FastQC (version 0.11.5)