##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631259.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1840639
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.07405960647362	34.0	33.0	34.0	31.0	34.0
2	33.21263430797674	34.0	33.0	34.0	31.0	34.0
3	33.29982305058189	34.0	34.0	34.0	31.0	34.0
4	36.52220125728076	37.0	37.0	37.0	35.0	37.0
5	36.509095482601424	37.0	37.0	37.0	35.0	37.0
6	36.55957632104938	37.0	37.0	37.0	35.0	37.0
7	36.56508527744984	37.0	37.0	37.0	35.0	37.0
8	36.56348528961953	37.0	37.0	37.0	35.0	37.0
9	38.41585992690582	39.0	39.0	39.0	37.0	39.0
10	38.380581960938564	39.0	39.0	39.0	37.0	39.0
11	38.43132575154607	39.0	39.0	39.0	37.0	39.0
12	38.38876987828684	39.0	39.0	39.0	37.0	39.0
13	38.41446095622227	39.0	39.0	39.0	37.0	39.0
14	39.968030124320954	41.0	40.0	41.0	38.0	41.0
15	39.96150575968454	41.0	40.0	41.0	38.0	41.0
16	39.934163081408144	41.0	40.0	41.0	38.0	41.0
17	39.922762149449184	41.0	40.0	41.0	38.0	41.0
18	39.896740751445556	41.0	40.0	41.0	38.0	41.0
19	39.912348917957296	41.0	40.0	41.0	38.0	41.0
20	39.8776636809282	41.0	40.0	41.0	38.0	41.0
21	39.85175039755215	41.0	40.0	41.0	38.0	41.0
22	39.797330709606825	41.0	40.0	41.0	38.0	41.0
23	39.73686475186063	41.0	40.0	41.0	38.0	41.0
24	39.71336856385201	41.0	40.0	41.0	37.0	41.0
25	39.7013939180904	41.0	40.0	41.0	37.0	41.0
26	39.60479702972717	41.0	40.0	41.0	37.0	41.0
27	39.55894556184021	41.0	40.0	41.0	37.0	41.0
28	39.48869821839046	41.0	40.0	41.0	37.0	41.0
29	39.440125412967994	41.0	39.0	41.0	36.0	41.0
30	39.371144477542856	41.0	39.0	41.0	36.0	41.0
31	39.31904083310198	41.0	39.0	41.0	35.0	41.0
32	39.25177995250562	41.0	39.0	41.0	35.0	41.0
33	39.17547058385702	41.0	39.0	41.0	35.0	41.0
34	39.12256993359372	41.0	39.0	41.0	35.0	41.0
35	39.025202117308176	41.0	39.0	41.0	35.0	41.0
36	38.96177957763581	41.0	39.0	41.0	35.0	41.0
37	38.90245452801989	41.0	39.0	41.0	35.0	41.0
38	38.82065956442301	40.0	38.0	41.0	35.0	41.0
39	38.73732491813984	40.0	38.0	41.0	35.0	41.0
40	38.638048525539226	40.0	38.0	41.0	35.0	41.0
41	38.5495194875258	40.0	38.0	41.0	35.0	41.0
42	38.41996773946439	40.0	38.0	41.0	35.0	41.0
43	37.623284631043894	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	3.0
13	0.0
14	6.0
15	3.0
16	4.0
17	13.0
18	26.0
19	63.0
20	107.0
21	223.0
22	375.0
23	605.0
24	1040.0
25	1573.0
26	2314.0
27	3330.0
28	4755.0
29	6617.0
30	8838.0
31	11542.0
32	15457.0
33	20485.0
34	31119.0
35	46330.0
36	76664.0
37	150320.0
38	343553.0
39	1115271.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.337286670553	17.752476178109884	11.966224773027195	26.94401237830992
2	19.45911175412452	19.49649007763065	33.297295124138955	27.74710304410588
3	19.970836215031845	21.32330131003418	28.59403717947952	30.11182529545446
4	14.653335064616147	15.154411049640911	33.403562567130216	36.78869131861272
5	16.5476771925402	34.35301544735279	32.32600200256541	16.77330535754159
6	35.192071883731685	33.961955603461625	15.464194771489684	15.38177774131701
7	31.14385819272546	29.10445774538082	20.145884119591077	19.605799942302646
8	28.77321408489117	31.29527300030044	19.23717795830687	20.69433495650152
9	27.62214643936155	13.898814487794727	18.98791669632122	39.491122376522505
10	19.004921660358168	24.55625464852152	30.30186799258301	26.1369556985373
11	38.40361961253673	20.53515110784896	20.08509001493503	20.97613926467928
12	23.728281319693867	24.444336993837464	26.770540013549642	25.056841672919024
13	31.231708118756586	17.835219182034066	23.767343840916116	27.165728858293235
14	23.93614391523813	19.555436997694823	23.30484141648634	33.20357767058071
15	27.15806847513282	25.738996076905902	20.719597922243306	26.38333752571797
16	26.929343559492114	24.357682304895203	22.497839065672302	26.215135069940388
17	25.632945949748976	24.83800462774069	23.545464374057055	25.983585048453286
18	25.93436301197573	23.101053492835913	24.892985533828195	26.071597961360162
19	26.282883281295245	23.89327836691497	24.37506757164224	25.448770780147544
20	26.427778613840086	23.081984028372755	24.18312336096323	26.30711399682393
21	27.83783240494198	23.53563083255326	23.66362985897832	24.962906903526438
22	27.40988319817194	23.140279000933912	23.542313294459152	25.907524506434992
23	25.999068801649862	23.035261123990093	24.26054212694613	26.705127947413914
24	25.854119140146437	24.04349793740109	23.70752765751459	26.394855264937885
25	26.135325829779767	23.012660277218945	24.058981690597665	26.793032202403623
26	25.962505412522496	24.16508614671318	24.25326204649581	25.619146394268512
27	26.563003391756883	23.25670595918048	23.67324608464778	26.507044564414855
28	25.474142403806503	23.674278334860883	24.768735205545468	26.082844055787145
29	25.38178317421287	23.82944184057819	24.915206077889255	25.873568907319687
30	25.014193440430198	24.212189353805933	24.957419678709407	25.816197527054463
31	26.047530232707228	23.82629076098029	23.853455240272538	26.272723766039945
32	25.01011876853636	23.41203245177354	24.323835363697064	27.254013415993033
33	24.64823357540506	23.00739036823625	25.355542287216558	26.98883376914213
34	25.97657661279588	22.802081233745454	25.107747907112692	26.113594246345972
35	25.86042129934224	22.947139553166046	25.50712008166729	25.685319065824423
36	24.757217466325553	23.39855887004459	25.217764048246288	26.626459615383567
37	25.00175210891435	22.258031042480354	25.897256333262526	26.842960515342767
38	24.29580162106747	21.753858306816273	26.982640267863495	26.967699804252764
39	24.394951970484165	21.48840701517245	27.26422726020692	26.85241375413647
40	24.043334950525335	21.949931518347704	27.888629981218475	26.118103549908483
41	22.923886758891886	21.842251522433244	28.941416540668758	26.29244517800612
42	23.39725497503856	22.234289287578935	28.67373776172297	25.694717975659543
43	22.304971262697357	21.298961936588327	28.98911736630594	27.40694943440838
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	124.0
1	137.5
2	151.0
3	407.5
4	664.0
5	664.0
6	850.5
7	1037.0
8	987.0
9	937.0
10	1470.5
11	2004.0
12	2004.0
13	3263.5
14	4523.0
15	6855.5
16	9188.0
17	8393.5
18	7599.0
19	7599.0
20	8560.5
21	9522.0
22	8243.0
23	6964.0
24	8157.5
25	9351.0
26	9351.0
27	11293.0
28	13235.0
29	16660.0
30	20085.0
31	24990.5
32	29896.0
33	29896.0
34	38672.5
35	47449.0
36	57102.0
37	66755.0
38	76263.5
39	85772.0
40	85772.0
41	92679.5
42	99587.0
43	105543.5
44	111500.0
45	116041.0
46	120582.0
47	120582.0
48	126183.0
49	131784.0
50	134735.5
51	137687.0
52	139169.5
53	140652.0
54	140652.0
55	137102.5
56	133553.0
57	128877.0
58	124201.0
59	121373.0
60	118545.0
61	118545.0
62	109907.5
63	101270.0
64	92843.0
65	84416.0
66	75349.5
67	66283.0
68	66283.0
69	58054.0
70	49825.0
71	43788.0
72	37751.0
73	30106.0
74	22461.0
75	22461.0
76	18343.0
77	14225.0
78	12305.5
79	10386.0
80	9027.5
81	7669.0
82	7669.0
83	6327.0
84	4985.0
85	4432.5
86	3880.0
87	3320.5
88	2761.0
89	2761.0
90	1933.0
91	1105.0
92	649.5
93	194.0
94	123.5
95	53.0
96	53.0
97	35.5
98	18.0
99	14.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1840639.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.346650244658555
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.61993435564813	47.07503276641693
2	7.493177623532323	8.144582070544258
3	2.146876193004119	3.5002658873933687
4	1.011885823882273	2.1997041983223222
5	0.5685128693610597	1.5448385035376386
6	0.3666535975915413	1.1955836897551968
7	0.2671210707659865	1.0161994784128427
8	0.18850801666082667	0.8195823399824171
9	0.14130593101874106	0.6911553609514233
>10	0.9506624110573523	10.136523617307668
>50	0.12471297354025264	4.755202235923662
>100	0.10581899076470079	12.029590341179262
>500	0.011523422059786969	4.182432481790191
>1k	0.0033067211128084348	2.7093070284829013
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4709	0.25583506597437083	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3642	0.19786606716471833	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3344	0.18167603750653985	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.432895858449158E-5
2	0.0	1.0865791716898316E-4	0.0	0.0	5.432895858449158E-5
3	0.0	1.6298687575347476E-4	0.0	0.0	5.432895858449158E-5
4	0.0	1.6298687575347476E-4	0.0	0.0	5.432895858449158E-5
5	0.0	2.1731583433796633E-4	0.0	0.0	5.432895858449158E-5
6	0.0	2.1731583433796633E-4	0.0	0.0	5.432895858449158E-5
7	0.0	2.1731583433796633E-4	0.0	0.0	5.432895858449158E-5
8	0.0	2.1731583433796633E-4	0.0	0.0	5.432895858449158E-5
9	0.0	2.1731583433796633E-4	0.0	0.0	5.432895858449158E-5
10	0.0	2.1731583433796633E-4	0.0	1.0865791716898316E-4	5.432895858449158E-5
11	0.0	2.1731583433796633E-4	0.0	3.8030271009144106E-4	5.432895858449158E-5
12	0.0	2.1731583433796633E-4	0.0	5.432895858449158E-4	5.432895858449158E-5
13	0.0	2.1731583433796633E-4	0.0	5.976185444294074E-4	5.432895858449158E-5
14	0.0	2.1731583433796633E-4	0.0	8.692633373518653E-4	5.432895858449158E-5
15	0.0	2.1731583433796633E-4	0.0	0.0011409081302743232	5.432895858449158E-5
16	0.0	2.1731583433796633E-4	0.0	0.0018471845918727138	5.432895858449158E-5
17	0.0	2.1731583433796633E-4	0.0	0.0022818162605486463	5.432895858449158E-5
18	0.0	2.1731583433796633E-4	0.0	0.0024448031363021212	1.0865791716898316E-4
19	0.0	2.1731583433796633E-4	0.0	0.0026621189706400876	1.0865791716898316E-4
20	0.0	2.1731583433796633E-4	0.0	0.0033140664736539863	1.0865791716898316E-4
21	0.0	2.1731583433796633E-4	0.0	0.00412900085242136	1.0865791716898316E-4
22	0.0	2.1731583433796633E-4	0.0	0.0058131985685405995	1.6298687575347476E-4
23	0.0	2.1731583433796633E-4	0.0	0.00825800170484272	1.6298687575347476E-4
24	0.0	2.716447929224579E-4	0.0	0.011843712971419165	1.6298687575347476E-4
25	0.0	2.716447929224579E-4	0.0	0.014288516107721286	1.6298687575347476E-4
26	0.0	2.716447929224579E-4	0.0	0.02026470155201536	1.6298687575347476E-4
27	0.0	2.716447929224579E-4	0.0	0.05655644588645574	1.6298687575347476E-4
28	0.0	2.716447929224579E-4	0.0	0.20416822636051937	1.6298687575347476E-4
29	0.0	3.259737515069495E-4	0.0	0.396873042459711	1.6298687575347476E-4
30	0.0	3.259737515069495E-4	0.0	0.6472752125756327	1.6298687575347476E-4
31	0.0	3.259737515069495E-4	0.0	1.291508003470534	1.6298687575347476E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATATAT	85	5.1850293E-8	21.764706	1
GGTATCA	1990	0.0	20.638191	1
ATAACGT	65	6.905012E-5	19.923077	17
AGTCGGT	440	0.0	18.920454	11
GCAGTCG	460	0.0	18.097824	9
CTTATAC	2715	0.0	17.64825	37
CGCCTTA	325	0.0	17.646154	25
ATACACA	600	0.0	17.575	37
TCGGTGA	510	0.0	17.411764	13
TAACGTT	75	2.068118E-4	17.266666	18
TATACTG	130	1.3946192E-8	17.076923	5
GTGATTC	500	0.0	17.02	16
CTCTATG	755	0.0	16.907286	1
CGGTGAT	515	0.0	16.883493	14
CAGTCGG	520	0.0	16.721153	10
GTATTAT	180	1.0913936E-11	16.444445	1
GTTCTAA	80	3.3839484E-4	16.1875	1
GTACTAT	220	0.0	15.977273	1
ATTAGAC	70	0.0025932887	15.857142	3
GGCAGTC	535	0.0	15.560747	8
>>END_MODULE