FastQCFastQC Report
Fri 10 Feb 2017
ERR1631258.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631258.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences792404
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA25060.3162528205309413No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC24260.3061569603384132No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC21110.266404510830334No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC17480.22059454520673796No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC16020.20216960035537426No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC15320.19333572268691224No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG13170.1662030984194931No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC12680.16001938405156965No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC12420.15673822948899804No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCT11930.1505545151210746No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG11690.1475257570633162No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC10880.13730369861838154No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG10870.13717750036597492No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG10710.13515832832746932No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG10630.13414874230821652No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT10070.12708164017344686No Hit
TAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGC9910.12506246813494126No Hit
GAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGG9900.12493626988253467No Hit
GCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGC9400.11862635726220462No Hit
GTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACAC9280.11711197823332542No Hit
GTCTAGAACTGATCCAGCAATTACAACGGAGTCAAAAATTAAA8740.11029727260336898No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC8230.10386116173063235No Hit
GTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGA8150.10285157571137955No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG556.257633E-726.909095
ATAGTGT358.8669546E-426.428576
GATGTAA451.322706E-424.6666641
GTCTAGA2500.023.681
ACGGAGT1800.023.63888726
CTAGAAC2750.023.5454563
AATCTAC400.00193066123.12514
ACTGATC1850.023.08
TCTAGAC1850.023.03
GACATGT1400.022.4642857
ATAATAC603.724405E-521.5833343
GCATTAG603.724405E-521.58333418
AACGGAG2000.021.27499825
AGTGCGC1059.822543E-1021.14285729
ACATGTA1400.021.1428578
GTGCGCT1059.822543E-1021.14285730
CTAGACA1950.020.8717964
GTCAAAA2750.020.85454631
TCGTTTA1600.020.812530
ACTGTTG450.003824776320.5555538
Produced by FastQC (version 0.11.5)