##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631258.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	792404
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.08777088454879	34.0	33.0	34.0	31.0	34.0
2	33.228431456681186	34.0	33.0	34.0	31.0	34.0
3	33.31422355263224	34.0	34.0	34.0	31.0	34.0
4	36.52941933660102	37.0	37.0	37.0	35.0	37.0
5	36.52549709491623	37.0	37.0	37.0	35.0	37.0
6	36.58034285541214	37.0	37.0	37.0	35.0	37.0
7	36.58507907582496	37.0	37.0	37.0	35.0	37.0
8	36.5787679012221	37.0	37.0	37.0	35.0	37.0
9	38.43803792005088	39.0	39.0	39.0	37.0	39.0
10	38.398255182962224	39.0	39.0	39.0	37.0	39.0
11	38.459836144189076	39.0	39.0	39.0	37.0	39.0
12	38.41934038697432	39.0	39.0	39.0	37.0	39.0
13	38.440968243471765	39.0	39.0	39.0	37.0	39.0
14	39.994693363486306	41.0	40.0	41.0	38.0	41.0
15	39.99870015800021	41.0	40.0	41.0	38.0	41.0
16	39.98157631713116	41.0	40.0	41.0	38.0	41.0
17	39.96756578714898	41.0	40.0	41.0	38.0	41.0
18	39.94827638426863	41.0	40.0	41.0	38.0	41.0
19	39.95506080231801	41.0	40.0	41.0	38.0	41.0
20	39.93927340094194	41.0	40.0	41.0	38.0	41.0
21	39.91311376520058	41.0	40.0	41.0	38.0	41.0
22	39.86290200453304	41.0	40.0	41.0	38.0	41.0
23	39.802088328680824	41.0	40.0	41.0	38.0	41.0
24	39.77438150236495	41.0	40.0	41.0	38.0	41.0
25	39.76126445600981	41.0	40.0	41.0	38.0	41.0
26	39.7000103482567	41.0	40.0	41.0	37.0	41.0
27	39.635659335389526	41.0	40.0	41.0	37.0	41.0
28	39.550272335828694	41.0	40.0	41.0	37.0	41.0
29	39.51735099772338	41.0	40.0	41.0	37.0	41.0
30	39.45613348746347	41.0	40.0	41.0	37.0	41.0
31	39.420390356434346	41.0	39.0	41.0	36.0	41.0
32	39.349550229428424	41.0	39.0	41.0	36.0	41.0
33	39.263605433591955	41.0	39.0	41.0	35.0	41.0
34	39.21446635806987	41.0	39.0	41.0	35.0	41.0
35	39.121680354970444	41.0	39.0	41.0	35.0	41.0
36	39.069546594918755	41.0	39.0	41.0	35.0	41.0
37	39.00265773519568	41.0	39.0	41.0	35.0	41.0
38	38.9285579073301	40.0	39.0	41.0	35.0	41.0
39	38.83379942554556	40.0	38.0	41.0	35.0	41.0
40	38.74549977031918	40.0	38.0	41.0	35.0	41.0
41	38.66159686220665	40.0	38.0	41.0	35.0	41.0
42	38.50987248928577	40.0	37.0	41.0	35.0	41.0
43	37.69681753247081	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	1.0
14	0.0
15	1.0
16	4.0
17	4.0
18	7.0
19	20.0
20	39.0
21	65.0
22	154.0
23	260.0
24	363.0
25	548.0
26	890.0
27	1214.0
28	1803.0
29	2403.0
30	3382.0
31	4259.0
32	5792.0
33	7932.0
34	12049.0
35	18627.0
36	32099.0
37	62458.0
38	153668.0
39	484361.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.91332704024715	16.81806250347045	10.991615388110105	27.276995068172294
2	20.903099933872117	19.23084689123225	31.344743337994256	28.521309836901377
3	21.35955901282679	19.976552364702854	27.887920808072646	30.77596781439771
4	14.636094719360326	14.114264945659032	32.67108697078763	38.57855336419301
5	16.89390765316682	34.706664782106095	31.47674671001156	16.922680854715523
6	37.288933422849965	32.53403566867406	14.88356949232967	15.29346141614631
7	32.24478927415813	28.39132563692258	18.784105077712884	20.579780011206406
8	30.5693812752081	29.079484707295773	18.538270882024825	21.8128631354713
9	28.20265924957471	12.831585908198342	17.604656210720794	41.36109863150615
10	18.843418256343984	24.324208358362654	30.22940318322472	26.602970202068644
11	40.00156485832984	19.868526660642804	18.61600900550729	21.513899475520063
12	24.85184325167465	23.602732949354117	25.463273784584633	26.082150014386603
13	32.7929944826124	16.73615983765857	23.679713883322144	26.79113179640688
14	24.734731273441326	18.5760041594944	21.804407852560058	34.88485671450422
15	28.366338382946072	25.02549204698613	19.608936855442426	26.999232714625364
16	28.35687351401558	23.54733191654762	21.08179665927986	27.013997910156938
17	27.388806719804542	23.77524596039394	21.444238040191618	27.391709279609895
18	27.767527675276753	21.129247202184747	23.505181700243817	27.598043422294687
19	27.20733363284385	22.85980383743646	22.235248686276194	27.6976138434435
20	27.33757022932746	21.444869031453653	22.894382158595867	28.323178580623015
21	29.101317004962112	22.14627891832954	22.814751061327303	25.93765301538104
22	29.293769339882182	21.589113633954398	21.65170796714807	27.46540905901535
23	27.681586665387858	21.529295662313668	22.609805099418985	28.17931257287949
24	26.501885401890956	22.70281321144265	22.49887683555358	28.296424551112814
25	27.607508291225187	21.955214764185946	22.53206697593652	27.905209968652358
26	27.941176470588236	22.77916315414864	21.95445757467151	27.32520280059162
27	28.34728244683268	21.470361078439787	22.81588684559896	27.366469629128577
28	26.870889091927857	21.989540688840542	23.149555024962016	27.990015194269592
29	26.026748981580106	22.173032947839737	23.47678709345233	28.323430977127828
30	25.09086274173275	23.557301578487742	24.35992246379372	26.991913215985786
31	27.82482168186935	22.787870833564696	21.832802459351544	27.55450502521441
32	27.007814195789017	22.342138606064584	22.507963109726855	28.142084088419544
33	26.16152871515035	21.872428710607217	23.241174956209207	28.724867618033223
34	27.506675887552305	21.91685049545434	23.251523212906548	27.324950404086806
35	26.645751409634478	21.935527836810518	24.31310291215087	27.105617841404133
36	25.908122624317897	23.079767391381164	23.736124502147895	27.275985482153043
37	27.462001706200372	21.37003346777654	24.25896386186844	26.90900096415465
38	25.694847577750746	20.804034305732934	25.093765301538106	28.40735281497822
39	26.978788597735498	21.18326005421477	24.61585251967431	27.222098828375422
40	25.602596655241516	21.40271881514985	25.05577962756372	27.938904902044914
41	25.245077006173616	21.545449038621715	26.192321088737565	27.017152866467104
42	26.195602243300137	20.953200640077537	26.549083548291026	26.302113568331304
43	23.773479184860246	20.43957375278267	26.42919520850475	29.357751853852328
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	10.0
1	13.0
2	16.0
3	48.0
4	80.0
5	80.0
6	106.5
7	133.0
8	127.5
9	122.0
10	175.0
11	228.0
12	228.0
13	427.5
14	627.0
15	991.0
16	1355.0
17	1308.5
18	1262.0
19	1262.0
20	1483.5
21	1705.0
22	1576.5
23	1448.0
24	1763.5
25	2079.0
26	2079.0
27	2592.0
28	3105.0
29	4032.5
30	4960.0
31	6189.5
32	7419.0
33	7419.0
34	9788.0
35	12157.0
36	15242.0
37	18327.0
38	21731.0
39	25135.0
40	25135.0
41	28182.0
42	31229.0
43	35006.0
44	38783.0
45	44152.5
46	49522.0
47	49522.0
48	54239.0
49	58956.0
50	60418.0
51	61880.0
52	64957.0
53	68034.0
54	68034.0
55	65586.5
56	63139.0
57	62369.0
58	61599.0
59	60135.0
60	58671.0
61	58671.0
62	57923.5
63	57176.0
64	55371.5
65	53567.0
66	48787.0
67	44007.0
68	44007.0
69	35393.0
70	26779.0
71	22614.0
72	18449.0
73	13059.5
74	7670.0
75	7670.0
76	5749.0
77	3828.0
78	3254.0
79	2680.0
80	2472.0
81	2264.0
82	2264.0
83	1883.5
84	1503.0
85	1302.0
86	1101.0
87	912.0
88	723.0
89	723.0
90	603.0
91	483.0
92	301.5
93	120.0
94	86.5
95	53.0
96	53.0
97	36.0
98	19.0
99	10.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	792404.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.778037051019545
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.81537314572947	35.01641764727314
2	7.385085973456723	6.170687908480795
3	2.6403831847371544	3.309300795625135
4	1.3473683621675168	2.2516162142402414
5	0.8377772371100057	1.75003442262413
6	0.5819428668838145	1.4587458392549122
7	0.4291287285097197	1.2549709143535184
8	0.30693050328903687	1.0258363150797967
9	0.25399774725889523	0.955037456627186
>10	1.911944341430261	16.335294716985153
>50	0.26852206946867385	7.831935392797732
>100	0.20214921375177392	15.561182727571884
>500	0.014547469655299577	4.1122880790410825
>1k	0.004849156551766525	2.966651570045341
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	2506	0.3162528205309413	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	2426	0.3061569603384132	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	2111	0.266404510830334	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	1748	0.22059454520673796	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	1602	0.20216960035537426	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	1532	0.19333572268691224	No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG	1317	0.1662030984194931	No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC	1268	0.16001938405156965	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1242	0.15673822948899804	No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCT	1193	0.1505545151210746	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG	1169	0.1475257570633162	No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC	1088	0.13730369861838154	No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG	1087	0.13717750036597492	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	1071	0.13515832832746932	No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG	1063	0.13414874230821652	No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT	1007	0.12708164017344686	No Hit
TAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGC	991	0.12506246813494126	No Hit
GAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGG	990	0.12493626988253467	No Hit
GCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGC	940	0.11862635726220462	No Hit
GTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACAC	928	0.11711197823332542	No Hit
GTCTAGAACTGATCCAGCAATTACAACGGAGTCAAAAATTAAA	874	0.11029727260336898	No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC	823	0.10386116173063235	No Hit
GTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGA	815	0.10285157571137955	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.2619825240660067E-4	0.0
10	0.0	0.0	0.0	1.2619825240660067E-4	0.0
11	0.0	0.0	0.0	1.2619825240660067E-4	0.0
12	0.0	0.0	0.0	2.5239650481320133E-4	0.0
13	0.0	0.0	0.0	2.5239650481320133E-4	0.0
14	0.0	0.0	0.0	3.78594757219802E-4	0.0
15	0.0	0.0	0.0	7.57189514439604E-4	0.0
16	0.0	0.0	0.0	0.0016405772812858087	0.0
17	0.0	0.0	0.0	0.0025239650481320135	0.0
18	0.0	0.0	0.0	0.003154956310165017	0.0
19	0.0	0.0	0.0	0.004290740581824423	0.0
20	0.0	0.0	0.0	0.004795533591450825	0.0
21	0.0	0.0	0.0	0.006183714367923433	0.0
22	0.0	0.0	0.0	0.007193300387176239	0.0
23	0.0	0.0	0.0	0.009843463687714852	0.0
24	0.0	0.0	0.0	0.013377014755099672	0.0
25	0.0	0.0	0.0	0.015396186793605281	0.0
26	0.0	0.0	0.0	0.023725271452440928	0.0
27	0.0	0.0	0.0	0.04315980232305743	0.0
28	0.0	0.0	0.0	0.10537554075951157	0.0
29	0.0	0.0	0.0	0.19270473142487923	0.0
30	0.0	0.0	0.0	0.30830233062932544	0.0
31	0.0	0.0	0.0	0.6031014482511446	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACTG	55	6.257633E-7	26.90909	5
ATAGTGT	35	8.8669546E-4	26.42857	6
GATGTAA	45	1.322706E-4	24.666664	1
GTCTAGA	250	0.0	23.68	1
ACGGAGT	180	0.0	23.638887	26
CTAGAAC	275	0.0	23.545456	3
AATCTAC	40	0.001930661	23.125	14
ACTGATC	185	0.0	23.0	8
TCTAGAC	185	0.0	23.0	3
GACATGT	140	0.0	22.464285	7
ATAATAC	60	3.724405E-5	21.583334	3
GCATTAG	60	3.724405E-5	21.583334	18
AACGGAG	200	0.0	21.274998	25
AGTGCGC	105	9.822543E-10	21.142857	29
ACATGTA	140	0.0	21.142857	8
GTGCGCT	105	9.822543E-10	21.142857	30
CTAGACA	195	0.0	20.871796	4
GTCAAAA	275	0.0	20.854546	31
TCGTTTA	160	0.0	20.8125	30
ACTGTTG	45	0.0038247763	20.555553	8
>>END_MODULE