FastQCFastQC Report
Fri 10 Feb 2017
ERR1631257.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631257.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences306198
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5260.17178427030875446No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4630.15120934819953102No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA4140.13520663100346836No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3900.1273685654380499No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC3310.10809998758972952No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC3210.10483412693747182No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC3100.10124168021998838No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGAT250.00549012529.613
TTAGCTC751.36533345E-824.6666684
ATTAGCT802.7092028E-823.1250023
TATTAGC855.149559E-821.7647062
TACACAT806.925857E-720.812537
AGGTTCC450.003819495220.55555710
CGCAAAG450.003819495220.55555718
GGTATCA4000.020.351
CTTATAC6450.018.93023137
GTATTAG1103.8242433E-818.51
GGACTGA500.007023386718.56
AAGCCTT500.007023386718.55
CAACTAA701.2154941E-418.526
TATACAC1851.8189894E-1217.037
TCTAGAA1005.849508E-616.6500029
AGCATTT1352.2006134E-816.44444523
ATACACA1251.6459671E-716.2836
ATAGCTC803.3724357E-416.187515
GAAAGCA1504.623871E-916.03333520
GTATCAA5100.015.9607842
Produced by FastQC (version 0.11.5)