FastQCFastQC Report
Fri 10 Feb 2017
ERR1631256.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631256.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2503407
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT74280.29671563593135275No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT68570.2739067199220902No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT58480.23360164767454913No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA36150.14440320730907918No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC30950.12363151497139697No Hit
GTGTAAGAGCAATTGCTGCCAGCATTCAAGTGCGCTGGGCCTG28850.11524294691194839No Hit
GTTCTAGACATGTATTTTCCAGCTGCCTCTAGTTTTTGAACTT27470.10973045933002504No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27360.1092910581459587No Hit
GTCTAGAACTGATCCAGCAATTACAACGGAGTCAAAAATTAAA25680.10258020369839982No Hit
GTGTGGGAGTGGGTGGGCAGTCTCACTGCTTGGAGCGTCCAGC25060.10010357884275309No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCACTA6550.021.18320712
TAGAACT8300.020.7289164
GCACTAT6700.020.43283713
CTAGAAC7800.020.3974343
CTAGACA7450.020.3624154
GGTATCA36450.020.0987661
TCTAGAC7550.020.0927143
ACTGATC5350.020.0560768
GTCTAGA7450.019.8657721
ACATGTA6100.019.1065588
ACTATTC7150.018.88811315
CTATTCA7350.018.62584916
CACTATT7600.018.514
TTACACT8100.018.2716054
CTTACAC7700.018.2597393
GTTCTAG9550.018.2094231
AGTCGGT7050.017.84397111
TCTTACA7800.017.788462
GACATGT6350.017.7716547
ATTACTA2950.017.55932420
Produced by FastQC (version 0.11.5)