FastQCFastQC Report
Fri 10 Feb 2017
ERR1631254.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631254.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68401
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT19872.904928290522068No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16102.3537667577959387No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT13541.9795032236370813No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA5110.7470651013874066No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4780.6988201926872414No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC2790.4078887735559421No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC2610.38157336881039755No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA2600.38011140188008946No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA2510.36695369950731715No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA2420.35379599713454485No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT2290.33479042704054035No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2270.3318664931799243No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT2140.31286092308591984No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA1830.2675399482463707No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1810.2646160143857546No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT1800.26315404745544657No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA1770.2587681466645224No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA1720.25145831201298224No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT1550.22660487419774566No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1360.19882750252189296No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA1330.19444160173096883No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT1270.18566980014912063No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1260.1842078332188126No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCC1230.17982193242788846No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG1180.1725120977763483No Hit
GCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA1140.16666423005511616No Hit
GGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATAGAGC1070.15643046154295992No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC1070.15643046154295992No Hit
CTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATA1070.15643046154295992No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA980.14327275917018756No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG910.13303899065803132No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT790.11549538749433487No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT790.11549538749433487No Hit
GCCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA730.10672358591248667No Hit
TCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCAT720.10526161898217863No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT720.10526161898217863No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCTGT700.10233768512156255No Hit
CATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGT700.10233768512156255No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACCTGTCT690.10087571819125453No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT690.10087571819125453No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCACTGTCT690.10087571819125453No Hit
TTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTGAT690.10087571819125453No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGACT251.221528E-437.031
TATTGAC303.564512E-430.83333230
GGCCAAG250.005460724229.61
TGACTGT358.7840465E-426.4285733
TTGACTG400.001912779523.12532
ATGCCCC753.6355414E-722.237
CAGTCGG3800.021.90789410
GCAGTCG3850.021.6233759
GGCAGTC3850.021.6233758
AGTCGGT3850.021.14285711
GGGCAGT3950.021.0759497
GCCTTAT3100.020.88709626
TTATACA806.765422E-720.812537
CGCCTTA3150.020.55555525
CACCTGT450.003789688220.55555535
TGGGCAG4050.020.5555556
CTCGCCT3200.020.23437523
TCGGTGA3950.020.1392413
TCGCCTT3300.019.62121224
GTCGGTG4200.019.3809512
Produced by FastQC (version 0.11.5)