##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631254.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	68401
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.94830484934431	34.0	33.0	34.0	31.0	34.0
2	33.03963392348065	34.0	33.0	34.0	31.0	34.0
3	33.02323065452259	34.0	33.0	34.0	31.0	34.0
4	36.35566731480534	37.0	37.0	37.0	35.0	37.0
5	36.3730354819374	37.0	37.0	37.0	35.0	37.0
6	36.49141094428444	37.0	37.0	37.0	35.0	37.0
7	36.49592842209909	37.0	37.0	37.0	35.0	37.0
8	36.52097191561527	37.0	37.0	37.0	35.0	37.0
9	38.334527272993085	39.0	39.0	39.0	37.0	39.0
10	38.2857121971901	39.0	39.0	39.0	37.0	39.0
11	38.356983085042614	39.0	39.0	39.0	37.0	39.0
12	38.33138404409292	39.0	39.0	39.0	37.0	39.0
13	38.33357699448838	39.0	39.0	39.0	37.0	39.0
14	39.70786976798585	41.0	40.0	41.0	37.0	41.0
15	39.663542930658906	41.0	39.0	41.0	37.0	41.0
16	39.55442171898072	41.0	39.0	41.0	37.0	41.0
17	39.56639522813994	41.0	39.0	41.0	37.0	41.0
18	39.73957983070423	41.0	40.0	41.0	38.0	41.0
19	39.79225449920323	41.0	40.0	41.0	38.0	41.0
20	39.82285346705458	41.0	40.0	41.0	38.0	41.0
21	39.82443239133931	41.0	40.0	41.0	38.0	41.0
22	39.730442537389806	41.0	40.0	41.0	37.0	41.0
23	39.65813365301677	41.0	40.0	41.0	37.0	41.0
24	39.6150933465885	41.0	40.0	41.0	37.0	41.0
25	39.5785441733308	41.0	40.0	41.0	37.0	41.0
26	39.40804958991828	41.0	39.0	41.0	36.0	41.0
27	39.26938202657856	41.0	39.0	41.0	35.0	41.0
28	39.132191049838454	41.0	39.0	41.0	35.0	41.0
29	38.93516176664084	40.0	38.0	41.0	35.0	41.0
30	38.669463896726654	40.0	37.0	41.0	35.0	41.0
31	38.38322539144165	40.0	37.0	41.0	35.0	41.0
32	38.148769754828145	40.0	36.0	41.0	35.0	41.0
33	37.942924811040776	40.0	35.0	41.0	35.0	41.0
34	37.78555869066242	40.0	35.0	41.0	35.0	41.0
35	37.59807605151971	40.0	35.0	41.0	34.0	41.0
36	37.40202628616541	40.0	35.0	41.0	34.0	41.0
37	37.18736568178828	40.0	35.0	41.0	33.0	41.0
38	36.93535182234178	39.0	35.0	41.0	33.0	41.0
39	36.630122366632065	39.0	35.0	41.0	33.0	41.0
40	36.3545269805997	39.0	35.0	41.0	32.0	41.0
41	35.99586263358723	39.0	35.0	41.0	31.0	41.0
42	35.59614625517171	38.0	35.0	41.0	29.0	41.0
43	34.63404043800529	37.0	35.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	2.0
14	1.0
15	0.0
16	4.0
17	0.0
18	2.0
19	1.0
20	10.0
21	20.0
22	27.0
23	39.0
24	63.0
25	112.0
26	159.0
27	242.0
28	370.0
29	530.0
30	677.0
31	849.0
32	1024.0
33	1246.0
34	1773.0
35	2640.0
36	4200.0
37	12026.0
38	13935.0
39	28448.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.617739506732356	13.493954766743176	15.929591672636365	23.9587140538881
2	20.18245347290244	21.14296574611482	33.340155845674765	25.334424935307965
3	20.53624947003699	23.176561746173302	30.484934430783177	25.80225435300654
4	13.167936141284484	18.176634844519818	31.13112381397933	37.524305200216375
5	13.988099589187291	40.06081782430081	28.752503618368152	17.198578968143742
6	28.242277159690648	30.99516088946068	21.02600839169018	19.736553559158494
7	30.44692329059517	32.25976228417713	16.955892457712608	20.337421967515095
8	36.873729916229294	26.85779447668894	15.929591672636365	20.338883934445402
9	32.16473443370711	11.80830689609801	16.110875571994562	39.91608309820032
10	22.031841639742108	26.96890396339235	21.239455563515154	29.759798833350388
11	34.0550576745954	26.983523632695427	14.315580181576292	24.64583851113288
12	25.637052089881728	30.812415023172175	23.115159135100363	20.435373751845734
13	36.85033844534436	18.087454861771025	23.762810485226822	21.299396207657782
14	24.98647680589465	21.645882370140786	24.88998698849432	28.47765383547024
15	33.36062338269908	23.859300302627155	20.223388546951067	22.556687767722693
16	24.239411704507244	24.685311618251195	25.821259923100538	25.254016754141023
17	19.928071227028845	30.48054852999225	25.637052089881728	23.954328153097176
18	22.96165260741802	21.568398122834463	29.5434277276648	25.92652154208272
19	22.411953041622198	28.97033669098405	30.144296136021403	18.473414131372348
20	25.369512141635358	21.732138419028963	32.43812224967471	20.46022718966097
21	28.7978245932077	20.66782649376471	30.129676466718326	20.404672446309263
22	26.911887253110333	21.878335112059766	26.274469671496032	24.93530796333387
23	22.99966374760603	24.730632593090746	27.267145217175187	25.002558442128038
24	21.67365974181664	24.653148345784416	31.445446703995554	22.227745208403384
25	21.0113887223871	24.173623192643383	28.303679770763583	26.511308314205934
26	24.854899782166928	23.033288987003115	28.325609274718204	23.786201956111753
27	22.561073668513618	23.195567316267308	27.148725895820235	27.094633119398836
28	20.632739287437317	24.176547126503998	30.353357407055455	24.83735617900323
29	21.27308080291224	25.027411879943273	31.920585956345665	21.77892136079882
30	20.10935512638704	26.23938246516864	33.9278665516586	19.723395856785718
31	20.730691071767957	27.54638090086402	29.436704141752312	22.28622388561571
32	19.689770617388636	26.794929898685695	33.41179222525986	20.103507258665807
33	21.378342421894416	24.78472536951214	33.32261224251107	20.51431996608237
34	22.199967836727534	26.419204397596523	28.196956184851096	23.183871580824842
35	20.245318050905688	28.61215479305858	28.45718629844593	22.6853408575898
36	17.75120246780018	27.746670370316224	31.125275946258096	23.376851215625503
37	19.099135977544186	28.756889519159078	29.84751684916887	22.296457654127863
38	19.331588719463166	24.4674785456353	32.505372728468885	23.695560006432654
39	20.666364526834403	24.51133755354454	32.040467244630925	22.78183067499013
40	19.60936243622169	23.666320667826493	30.449847224455784	26.274469671496032
41	16.508530577038346	25.21746758088332	33.46734696861157	24.806654873466762
42	17.008523267203696	23.808131460066374	32.90595166737329	26.277393605356647
43	15.454452420286252	26.208681159632167	34.442478911127026	23.894387508954548
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	5.0
2	10.0
3	49.0
4	88.0
5	88.0
6	132.5
7	177.0
8	191.0
9	205.0
10	335.0
11	465.0
12	465.0
13	917.0
14	1369.0
15	2451.0
16	3533.0
17	3102.0
18	2671.0
19	2671.0
20	2895.0
21	3119.0
22	2108.0
23	1097.0
24	944.5
25	792.0
26	792.0
27	748.0
28	704.0
29	760.5
30	817.0
31	811.5
32	806.0
33	806.0
34	775.0
35	744.0
36	713.0
37	682.0
38	795.5
39	909.0
40	909.0
41	1148.5
42	1388.0
43	1841.0
44	2294.0
45	3039.0
46	3784.0
47	3784.0
48	5711.5
49	7639.0
50	7216.0
51	6793.0
52	5557.0
53	4321.0
54	4321.0
55	3567.0
56	2813.0
57	2824.5
58	2836.0
59	2824.0
60	2812.0
61	2812.0
62	2862.5
63	2913.0
64	2913.0
65	2913.0
66	2778.5
67	2644.0
68	2644.0
69	2467.5
70	2291.0
71	2061.0
72	1831.0
73	1572.0
74	1313.0
75	1313.0
76	1057.0
77	801.0
78	622.5
79	444.0
80	336.0
81	228.0
82	228.0
83	161.0
84	94.0
85	69.0
86	44.0
87	27.0
88	10.0
89	10.0
90	7.0
91	4.0
92	3.0
93	2.0
94	1.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	68401.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.55608836128127
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.38085708370713	58.078098273417055
2	4.632760564028247	5.888802795280771
3	1.3433625468681711	2.5613660618996796
4	0.6831826651024774	1.736816713205947
5	0.40024843006003724	1.2719112293679917
6	0.21852644169944566	0.8333211502755807
7	0.17942171002691326	0.7982339439481879
8	0.11271363835024038	0.5730910366807502
9	0.15411864835645112	0.8815660589757459
>10	0.7521910151128287	9.416528998114062
>50	0.07590918501138638	3.1227613631379656
>100	0.057506958341959374	6.852239002353768
>500	0.002300278333678375	0.7470651013874066
>1k	0.006900835001035125	7.238198271955089
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1987	2.904928290522068	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1610	2.3537667577959387	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1354	1.9795032236370813	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	511	0.7470651013874066	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	478	0.6988201926872414	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC	279	0.4078887735559421	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC	261	0.38157336881039755	No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA	260	0.38011140188008946	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	251	0.36695369950731715	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA	242	0.35379599713454485	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT	229	0.33479042704054035	No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	227	0.3318664931799243	No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT	214	0.31286092308591984	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	183	0.2675399482463707	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	181	0.2646160143857546	No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT	180	0.26315404745544657	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA	177	0.2587681466645224	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA	172	0.25145831201298224	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT	155	0.22660487419774566	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	136	0.19882750252189296	No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA	133	0.19444160173096883	No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT	127	0.18566980014912063	No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	126	0.1842078332188126	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCC	123	0.17982193242788846	No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG	118	0.1725120977763483	No Hit
GCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA	114	0.16666423005511616	No Hit
GGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATAGAGC	107	0.15643046154295992	No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC	107	0.15643046154295992	No Hit
CTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATA	107	0.15643046154295992	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	98	0.14327275917018756	No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG	91	0.13303899065803132	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	79	0.11549538749433487	No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT	79	0.11549538749433487	No Hit
GCCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	73	0.10672358591248667	No Hit
TCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCAT	72	0.10526161898217863	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT	72	0.10526161898217863	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCTGT	70	0.10233768512156255	No Hit
CATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGT	70	0.10233768512156255	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACCTGTCT	69	0.10087571819125453	No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT	69	0.10087571819125453	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCACTGTCT	69	0.10087571819125453	No Hit
TTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTGAT	69	0.10087571819125453	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.002923933860616073	0.0
16	0.0	0.0	0.0	0.005847867721232146	0.0
17	0.0	0.0	0.0	0.005847867721232146	0.0
18	0.0	0.0	0.0	0.014619669303080365	0.0
19	0.0	0.0	0.0	0.019005570094004474	0.0
20	0.0	0.0	0.0	0.023391470884928583	0.0
21	0.0	0.0	0.0	0.03362523939708484	0.0
22	0.0	0.0	0.0	0.04532097483954913	0.0
23	0.0	0.0	0.0	0.06725047879416968	0.0
24	0.0	0.0	0.0	0.09502785047002237	0.0
25	0.0	0.0	0.0	0.10672358591248667	0.0
26	0.0	0.0	0.0	0.11988128828525899	0.0014619669303080364
27	0.0	0.0	0.0	0.1681261969854242	0.0014619669303080364
28	0.0	0.0	0.0	0.31139895615561175	0.0014619669303080364
29	0.0	0.0	0.0	0.564319235098902	0.0014619669303080364
30	0.0	0.0	0.0	0.9663601409336121	0.0014619669303080364
31	0.0	0.0	0.0	1.6841859037148579	0.0014619669303080364
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTGACT	25	1.221528E-4	37.0	31
TATTGAC	30	3.564512E-4	30.833332	30
GGCCAAG	25	0.0054607242	29.6	1
TGACTGT	35	8.7840465E-4	26.42857	33
TTGACTG	40	0.0019127795	23.125	32
ATGCCCC	75	3.6355414E-7	22.2	37
CAGTCGG	380	0.0	21.907894	10
GCAGTCG	385	0.0	21.623375	9
GGCAGTC	385	0.0	21.623375	8
AGTCGGT	385	0.0	21.142857	11
GGGCAGT	395	0.0	21.075949	7
GCCTTAT	310	0.0	20.887096	26
TTATACA	80	6.765422E-7	20.8125	37
CGCCTTA	315	0.0	20.555555	25
CACCTGT	45	0.0037896882	20.555555	35
TGGGCAG	405	0.0	20.555555	6
CTCGCCT	320	0.0	20.234375	23
TCGGTGA	395	0.0	20.13924	13
TCGCCTT	330	0.0	19.621212	24
GTCGGTG	420	0.0	19.38095	12
>>END_MODULE