FastQCFastQC Report
Fri 10 Feb 2017
ERR1631253.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631253.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences887931
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC24640.27749903990287533No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT22540.2538485535475166No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT22200.25001942718522047No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT20360.22929709628338235No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT15760.17749126902878715No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA13320.15001165631113228No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC11470.12917670404569725No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG11400.1283883545005186No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG11310.12737476222814612No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT11260.12681165541016137No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG11170.12579806313778888No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC11000.12388349995664077No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT10980.1236582572294469No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT10430.11746408223161484No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC10370.11678835405003318No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG9250.1041747613271752No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8650.025.664741
TTAGGAC1001.288754E-820.353
CAGGACA6700.019.6044774
TATACAC3550.019.2816937
TGCATCA6050.018.9586814
GCTGCAT6100.018.8032812
CTGCATC6250.018.64813
TGTATGG500.00703365218.52
GGTCAGA701.2188012E-418.51
GTACTAT1002.8730938E-718.51
GCATCAG6500.018.515
ATACACA2100.018.537
GACAGGC6350.018.062997
TCCAGGA7500.017.5133322
CCAGGAC7800.017.3141023
TCGTTTA2250.017.26666630
TCTAGCC650.001579550917.0769233
ACAGGCT6950.016.7697858
ATCAGAA6850.016.74452617
GAGTGTT1452.9758667E-916.5862067
Produced by FastQC (version 0.11.5)