##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631253.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	887931
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.043629516257454	34.0	33.0	34.0	31.0	34.0
2	33.19237080358722	34.0	33.0	34.0	31.0	34.0
3	33.26639006859767	34.0	34.0	34.0	31.0	34.0
4	36.5003068932158	37.0	37.0	37.0	35.0	37.0
5	36.49178370841879	37.0	37.0	37.0	35.0	37.0
6	36.539066661711324	37.0	37.0	37.0	35.0	37.0
7	36.55044141943462	37.0	37.0	37.0	35.0	37.0
8	36.54172565210585	37.0	37.0	37.0	35.0	37.0
9	38.38511776252884	39.0	39.0	39.0	37.0	39.0
10	38.35486653805307	39.0	39.0	39.0	37.0	39.0
11	38.405369336130846	39.0	39.0	39.0	37.0	39.0
12	38.3648459170814	39.0	39.0	39.0	37.0	39.0
13	38.382178344938964	39.0	39.0	39.0	37.0	39.0
14	39.92601564761226	41.0	40.0	41.0	38.0	41.0
15	39.9194261716282	41.0	40.0	41.0	38.0	41.0
16	39.88917044229788	41.0	40.0	41.0	38.0	41.0
17	39.87089537362701	41.0	40.0	41.0	38.0	41.0
18	39.7934524191632	41.0	40.0	41.0	38.0	41.0
19	39.81384251704243	41.0	40.0	41.0	38.0	41.0
20	39.794401817258326	41.0	40.0	41.0	38.0	41.0
21	39.766017854990984	41.0	40.0	41.0	38.0	41.0
22	39.70789059059769	41.0	40.0	41.0	37.0	41.0
23	39.6410903549938	41.0	40.0	41.0	37.0	41.0
24	39.6077228973873	41.0	40.0	41.0	37.0	41.0
25	39.58986114912082	41.0	40.0	41.0	37.0	41.0
26	39.48279314496284	41.0	40.0	41.0	36.0	41.0
27	39.435320987779455	41.0	39.0	41.0	36.0	41.0
28	39.34580164449715	41.0	39.0	41.0	36.0	41.0
29	39.28234288475118	41.0	39.0	41.0	35.0	41.0
30	39.21112000819883	41.0	39.0	41.0	35.0	41.0
31	39.17025196777678	41.0	39.0	41.0	35.0	41.0
32	39.09238893562676	41.0	39.0	41.0	35.0	41.0
33	38.99502664058356	41.0	39.0	41.0	35.0	41.0
34	38.924676579599094	41.0	39.0	41.0	35.0	41.0
35	38.79982340970188	40.0	38.0	41.0	35.0	41.0
36	38.74223109678567	40.0	38.0	41.0	35.0	41.0
37	38.67027730758358	40.0	38.0	41.0	35.0	41.0
38	38.5893250714301	40.0	38.0	41.0	35.0	41.0
39	38.493315358963706	40.0	38.0	41.0	35.0	41.0
40	38.368286499739284	40.0	37.0	41.0	35.0	41.0
41	38.25648839831023	40.0	37.0	41.0	34.0	41.0
42	38.132040665321966	40.0	37.0	41.0	34.0	41.0
43	37.29762447757765	39.0	35.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	0.0
13	1.0
14	2.0
15	5.0
16	5.0
17	7.0
18	17.0
19	37.0
20	57.0
21	116.0
22	229.0
23	356.0
24	564.0
25	865.0
26	1235.0
27	1819.0
28	2584.0
29	3561.0
30	4639.0
31	6023.0
32	7913.0
33	10871.0
34	17685.0
35	28305.0
36	42267.0
37	77648.0
38	171551.0
39	509567.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.2100793867992	16.42244723970669	11.365072286022224	28.002401087471885
2	20.917391103588002	18.29590362314189	31.909010947922756	28.877694325347353
3	21.657313462419943	19.944455143473984	27.40607096722606	30.992160426880016
4	15.931755958514795	14.303926769084535	31.09081674139094	38.67350053100973
5	17.6242298106497	33.53909256462496	30.741127407422425	18.09555021730292
6	37.1219160047346	31.217065289983122	15.018509321107157	16.64250938417512
7	32.37548863594131	27.694944764852224	18.743460922076153	21.186105677130318
8	30.39098758799952	29.55376037101982	18.23689002861709	21.818362012363572
9	28.509647709112535	13.197534493108135	17.68515796835565	40.60765982942368
10	20.60779497506	23.63776014127224	28.54151955501047	27.212925328657295
11	38.962486949999494	19.99299495118427	18.666765773466633	22.377752325349604
12	25.430241764281234	23.051565943750134	24.897993199922066	26.620199092046565
13	32.36704203367153	16.739363756868496	22.86686690745114	28.026727302008826
14	24.881775723564107	18.727468688445388	21.903729005970057	34.487026582020455
15	28.732975873125277	24.17586501653845	19.45522794000885	27.635931170327428
16	28.072564196992783	22.945589240605408	20.77560080681945	28.20624575558236
17	27.023383573723635	23.563542662661852	22.233934844036305	27.17913891957821
18	26.79690201153018	21.046004700815715	23.801624225305794	28.355469062348316
19	27.448303978574913	22.59815233390883	22.736563989769476	27.216979697746783
20	27.31259523544059	21.91656784142011	22.772602826120497	27.9982340970188
21	30.132183694453733	21.736711523755787	22.037072700468844	26.094032081321632
22	29.669309890070288	21.79043191419153	21.56766685699677	26.97259133874141
23	27.942486522038312	21.89967463688057	22.01837755411175	28.139461286969368
24	27.508669029462872	22.39228048125361	22.439919318055118	27.659131171228395
25	27.451457376755627	21.48218724202669	22.61144165481327	28.454913726404417
26	28.03033118564393	22.629911558443165	22.9347775897001	26.404979666212803
27	28.838051605361226	21.37519694660959	22.023445515473615	27.76330593255557
28	26.770435991084895	21.558319283818225	23.63776014127224	28.03348458382464
29	26.607472877960113	21.76711929192696	24.06436986657747	27.561037963535455
30	25.878700034124275	22.55186495347048	24.150863073819924	27.418571938585316
31	27.098502023242798	22.534070778022166	22.52235815620808	27.845069042526955
32	26.27355053489517	21.991686290939274	23.49270382495937	28.24205934920619
33	26.824381624247827	22.033694059560936	23.262393136403617	27.87953117978762
34	27.598653498976834	22.050925128191267	23.28029993321553	27.07012143961637
35	27.450669027210452	21.842688226900513	24.193208706532378	26.51343403935666
36	26.11081266449758	22.6803659293346	23.172859152344046	28.035962253823776
37	26.032653438161297	21.393779471603086	24.469581532799285	28.103985557436335
38	25.99785343680984	20.727398863199955	25.414362152014064	27.860385547976136
39	25.33000875067995	20.73471925183376	25.773286437797534	28.161985559688755
40	25.375733024300313	20.211593017925942	26.90929813239993	27.503375825373816
41	23.77369412713375	20.626715364144285	28.008032155651737	27.591558353070226
42	24.302901914675804	20.95624547402895	27.42904572539984	27.31180688589541
43	23.143690219172434	19.27706094279848	28.5625797500031	29.01666908802598
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	81.0
1	75.5
2	70.0
3	150.5
4	231.0
5	231.0
6	279.5
7	328.0
8	337.0
9	346.0
10	524.5
11	703.0
12	703.0
13	1246.0
14	1789.0
15	2981.5
16	4174.0
17	3824.5
18	3475.0
19	3475.0
20	4018.0
21	4561.0
22	3820.5
23	3080.0
24	3425.0
25	3770.0
26	3770.0
27	4415.0
28	5060.0
29	6397.0
30	7734.0
31	9492.5
32	11251.0
33	11251.0
34	14129.0
35	17007.0
36	20613.5
37	24220.0
38	27164.0
39	30108.0
40	30108.0
41	32791.0
42	35474.0
43	38124.0
44	40774.0
45	43781.0
46	46788.0
47	46788.0
48	50423.5
49	54059.0
50	57101.5
51	60144.0
52	64978.5
53	69813.0
54	69813.0
55	69603.0
56	69393.0
57	67157.5
58	64922.0
59	61908.5
60	58895.0
61	58895.0
62	58221.0
63	57547.0
64	54235.0
65	50923.0
66	45209.0
67	39495.0
68	39495.0
69	34540.0
70	29585.0
71	27281.0
72	24977.0
73	23944.0
74	22911.0
75	22911.0
76	21675.0
77	20439.0
78	16427.0
79	12415.0
80	8548.0
81	4681.0
82	4681.0
83	3676.0
84	2671.0
85	2244.0
86	1817.0
87	1582.5
88	1348.0
89	1348.0
90	1000.0
91	652.0
92	400.0
93	148.0
94	97.5
95	47.0
96	47.0
97	30.0
98	13.0
99	12.5
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	887931.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.76165849386218
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.10874208911996	42.963283920734476
2	5.977267231755097	5.829229269627899
3	1.7123776679379028	2.504951251695124
4	0.8696280482899943	1.6961802362960237
5	0.5623394013601657	1.371030092338365
6	0.3793228070523466	1.109784550585182
7	0.2877819164933518	0.9822906472930586
8	0.21799468721026175	0.850382599297847
9	0.1759751476770443	0.7722756048991497
>10	1.3489215113552278	13.226365916384408
>50	0.17623085234165317	5.96386532702458
>100	0.1692738391495914	16.940637091386186
>500	0.010666570685899672	3.322434041632426
>1k	0.003478229571489023	2.4672894508052394
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	2464	0.27749903990287533	No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT	2254	0.2538485535475166	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2220	0.25001942718522047	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2036	0.22929709628338235	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1576	0.17749126902878715	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1332	0.15001165631113228	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	1147	0.12917670404569725	No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG	1140	0.1283883545005186	No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG	1131	0.12737476222814612	No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT	1126	0.12681165541016137	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	1117	0.12579806313778888	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	1100	0.12388349995664077	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	1098	0.1236582572294469	No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT	1043	0.11746408223161484	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	1037	0.11678835405003318	No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG	925	0.1041747613271752	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	1.1262136359694616E-4	0.0	0.0	0.0
6	0.0	1.1262136359694616E-4	0.0	0.0	0.0
7	0.0	1.1262136359694616E-4	0.0	0.0	0.0
8	0.0	1.1262136359694616E-4	0.0	0.0	0.0
9	0.0	1.1262136359694616E-4	0.0	0.0	0.0
10	0.0	1.1262136359694616E-4	0.0	0.0	1.1262136359694616E-4
11	0.0	1.1262136359694616E-4	0.0	0.0	1.1262136359694616E-4
12	0.0	1.1262136359694616E-4	0.0	0.0	1.1262136359694616E-4
13	0.0	1.1262136359694616E-4	0.0	0.0	1.1262136359694616E-4
14	0.0	1.1262136359694616E-4	0.0	0.0	1.1262136359694616E-4
15	0.0	1.1262136359694616E-4	0.0	2.2524272719389232E-4	1.1262136359694616E-4
16	0.0	1.1262136359694616E-4	0.0	6.75728181581677E-4	4.5048545438778465E-4
17	0.0	1.1262136359694616E-4	0.0	9.009709087755693E-4	4.5048545438778465E-4
18	0.0	1.1262136359694616E-4	0.0	0.0010135922723725154	4.5048545438778465E-4
19	0.0	1.1262136359694616E-4	0.0	0.0012388349995664078	4.5048545438778465E-4
20	0.0	1.1262136359694616E-4	0.0	0.0015766990903572462	5.631068179847308E-4
21	0.0	1.1262136359694616E-4	0.0	0.002252427271938923	5.631068179847308E-4
22	0.0	1.1262136359694616E-4	0.0	0.003040776817117546	5.631068179847308E-4
23	0.0	1.1262136359694616E-4	0.0	0.005067961361862577	5.631068179847308E-4
24	0.0	1.1262136359694616E-4	0.0	0.007320388633801501	5.631068179847308E-4
25	0.0	1.1262136359694616E-4	0.0	0.009910679996531262	5.631068179847308E-4
26	0.0	1.1262136359694616E-4	0.0	0.019258253175077792	5.631068179847308E-4
27	0.0	1.1262136359694616E-4	0.0	0.055522332253294455	5.631068179847308E-4
28	0.0	2.2524272719389232E-4	0.0	0.1740000067572818	5.631068179847308E-4
29	0.0	2.2524272719389232E-4	0.0	0.33144467306581255	5.631068179847308E-4
30	0.0	2.2524272719389232E-4	0.0	0.5290951661784531	5.631068179847308E-4
31	0.0	2.2524272719389232E-4	0.0	0.9857747955640698	5.631068179847308E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	865	0.0	25.66474	1
TTAGGAC	100	1.288754E-8	20.35	3
CAGGACA	670	0.0	19.604477	4
TATACAC	355	0.0	19.28169	37
TGCATCA	605	0.0	18.95868	14
GCTGCAT	610	0.0	18.80328	12
CTGCATC	625	0.0	18.648	13
TGTATGG	50	0.007033652	18.5	2
GGTCAGA	70	1.2188012E-4	18.5	1
GTACTAT	100	2.8730938E-7	18.5	1
GCATCAG	650	0.0	18.5	15
ATACACA	210	0.0	18.5	37
GACAGGC	635	0.0	18.06299	7
TCCAGGA	750	0.0	17.513332	2
CCAGGAC	780	0.0	17.314102	3
TCGTTTA	225	0.0	17.266666	30
TCTAGCC	65	0.0015795509	17.076923	3
ACAGGCT	695	0.0	16.769785	8
ATCAGAA	685	0.0	16.744526	17
GAGTGTT	145	2.9758667E-9	16.586206	7
>>END_MODULE