FastQCFastQC Report
Fri 10 Feb 2017
ERR1631251.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631251.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1161008
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13080.1126607224067362No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA12780.11007676088364594No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8500.023.070591
ACATGTA1450.021.6896558
CCCTTAA603.726328E-521.5833341
TAGAACT2850.020.1228084
GTTCTAG2350.018.8936161
TGTTATG500.00703492518.4999982
GTATAGT500.00703492518.4999981
TACTAGG500.00703492518.4999982
ATATTAC500.00703492518.4999981
TCTAGAC2450.018.1224483
GTATCAA10550.017.360192
GACATGT2250.017.2666667
GTCTAGA3250.017.0769231
CTAGACA2500.017.024
GTAGACC2950.016.3050844
GTAGAAT803.382601E-416.18753
CTAGAAC3150.015.8571423
TTAGAAC1059.3431845E-615.8571423
ACGGAGT2100.015.85714226
CTCTAGT2450.015.85714227
Produced by FastQC (version 0.11.5)