##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631251.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1161008
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.09223622920772	34.0	33.0	34.0	31.0	34.0
2	33.23107506580489	34.0	33.0	34.0	31.0	34.0
3	33.31786602676295	34.0	34.0	34.0	31.0	34.0
4	36.53562852280088	37.0	37.0	37.0	35.0	37.0
5	36.51909547565564	37.0	37.0	37.0	35.0	37.0
6	36.5667075506801	37.0	37.0	37.0	35.0	37.0
7	36.57378846657387	37.0	37.0	37.0	35.0	37.0
8	36.57026480437688	37.0	37.0	37.0	35.0	37.0
9	38.429116767498584	39.0	39.0	39.0	37.0	39.0
10	38.39404896434822	39.0	39.0	39.0	37.0	39.0
11	38.44973936441437	39.0	39.0	39.0	37.0	39.0
12	38.41289379573612	39.0	39.0	39.0	37.0	39.0
13	38.43014518418478	39.0	39.0	39.0	37.0	39.0
14	39.99570114934608	41.0	40.0	41.0	38.0	41.0
15	39.98791825723854	41.0	40.0	41.0	38.0	41.0
16	39.97193128729518	41.0	40.0	41.0	38.0	41.0
17	39.95917771453771	41.0	40.0	41.0	38.0	41.0
18	39.93764642448631	41.0	40.0	41.0	38.0	41.0
19	39.94632336728085	41.0	40.0	41.0	38.0	41.0
20	39.910307250251506	41.0	40.0	41.0	38.0	41.0
21	39.8791963535135	41.0	40.0	41.0	38.0	41.0
22	39.835948589501534	41.0	40.0	41.0	38.0	41.0
23	39.78449071841021	41.0	40.0	41.0	38.0	41.0
24	39.754062848834806	41.0	40.0	41.0	38.0	41.0
25	39.7394023124733	41.0	40.0	41.0	38.0	41.0
26	39.658071262213525	41.0	40.0	41.0	37.0	41.0
27	39.6097132836294	41.0	40.0	41.0	37.0	41.0
28	39.55713569587807	41.0	40.0	41.0	37.0	41.0
29	39.523700956410295	41.0	40.0	41.0	37.0	41.0
30	39.45695206234582	41.0	40.0	41.0	36.0	41.0
31	39.42209356007883	41.0	39.0	41.0	36.0	41.0
32	39.35111472100106	41.0	39.0	41.0	36.0	41.0
33	39.278123837217315	41.0	39.0	41.0	35.0	41.0
34	39.22681841985585	41.0	39.0	41.0	35.0	41.0
35	39.14950284580296	41.0	39.0	41.0	35.0	41.0
36	39.08978404972231	41.0	39.0	41.0	35.0	41.0
37	39.04186965119965	41.0	39.0	41.0	35.0	41.0
38	38.974902843046735	41.0	39.0	41.0	35.0	41.0
39	38.90191884982705	40.0	39.0	41.0	35.0	41.0
40	38.82184015958546	40.0	38.0	41.0	35.0	41.0
41	38.74262192853107	40.0	38.0	41.0	35.0	41.0
42	38.61944792800739	40.0	38.0	41.0	35.0	41.0
43	37.82367132698483	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	4.0
12	1.0
13	0.0
14	1.0
15	0.0
16	3.0
17	8.0
18	25.0
19	18.0
20	57.0
21	106.0
22	206.0
23	337.0
24	620.0
25	857.0
26	1234.0
27	1971.0
28	2694.0
29	3723.0
30	5133.0
31	6774.0
32	8924.0
33	11710.0
34	17743.0
35	27507.0
36	45613.0
37	87292.0
38	217489.0
39	720956.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.508220442925456	16.88403525212574	11.108192191612805	27.499552113335994
2	20.403907638879318	19.310461254358284	32.163172002260104	28.122459104502294
3	20.947745407439054	20.003824263054174	28.053295067734247	30.99513526177253
4	15.609280900734534	14.259247136970632	31.815370781252156	38.316101181042676
5	17.273868913909293	33.61475545388146	31.858350674586223	17.25302495762303
6	37.52885423700784	31.807532764632114	14.79903669914419	15.864576299215855
7	32.202792745614154	28.01444951283712	19.315973705607544	20.46678403594118
8	29.122366081887463	30.525715585077794	18.94663947190717	21.40527886112757
9	27.759498642558878	13.196033102269752	18.34276766396097	40.7017005912104
10	18.806588757355676	24.541691357854553	30.479893334068326	26.171826550721445
11	38.80171368328212	19.93845003651999	19.461536871408295	21.798299408789603
12	24.423776580350868	23.185369954384466	26.621005195485303	25.769848269779366
13	32.399949009825946	17.45026735388559	23.002080950346596	27.147702685941873
14	24.616195581770324	18.75775188456927	22.434470735774433	34.19158179788597
15	27.51083541198682	25.476051844603997	20.166010914653473	26.847101828755704
16	27.777672505271283	23.469002797569008	21.60019569201935	27.153129005140357
17	26.14366137011976	24.29561208880559	22.831539489822635	26.72918705125202
18	26.86570635172195	22.4220677204636	23.998370381599436	26.713855546215015
19	26.851063903091106	23.015689814368205	23.33825434450064	26.79499193804005
20	26.940210575637725	22.74566583520527	23.727829610131884	26.586293979025122
21	28.225817565425903	22.754795832586854	23.2750334192357	25.74435318275154
22	28.075086476578974	22.486580626490085	22.30949313010763	27.128839766823308
23	26.76208949464603	22.629215302564667	23.285541529429597	27.323153673359702
24	26.56588068299271	22.98209831456803	22.95031558783402	27.501705414605237
25	27.34735677962598	22.571506828548983	23.07296762813004	27.008168763694997
26	27.085946005540013	23.074431872993124	22.931624932816998	26.907997188649862
27	27.14985598721111	22.454970163857613	23.270037760291057	27.125136088640218
28	26.254513319460333	22.729042349406722	23.728346402436504	27.28809792869644
29	26.21127502997395	22.90156484709838	23.53213759078318	27.35502253214448
30	25.674844617780412	23.30621322161432	24.318350950208785	26.700591210396485
31	27.22728870085305	23.00070283753428	22.87279674214131	26.899211719471356
32	26.221007951710927	22.717586786654355	23.55926918677563	27.50213607485909
33	26.05572054628392	22.46375563303612	23.7721014842275	27.70842233645246
34	26.79430288163389	22.09993385058501	23.77339346498904	27.332369802792055
35	26.15675344183675	22.364273114397147	24.777262516709616	26.70171092705649
36	25.6669204691096	22.35471245676171	24.696384521036894	27.281982553091794
37	26.27053388090349	21.5864145638962	25.07949988286041	27.063551672339898
38	25.294916141835373	21.23594324931439	26.04917451042542	27.41996609842482
39	25.705766024006728	21.05532433885038	26.204815126166224	27.03409451097667
40	25.130317792814523	21.911735319653268	26.048657718120804	26.90928916941141
41	24.690010749279935	21.269190220911483	27.35502253214448	26.685776497664097
42	24.74789148739716	21.229138817303582	27.269148877527115	26.753820817772144
43	23.284507944820362	20.928451828066645	27.41410911897248	28.372931108140513
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	172.0
1	160.5
2	149.0
3	279.5
4	410.0
5	410.0
6	464.5
7	519.0
8	449.5
9	380.0
10	561.0
11	742.0
12	742.0
13	1241.5
14	1741.0
15	2287.5
16	2834.0
17	2711.0
18	2588.0
19	2588.0
20	3062.0
21	3536.0
22	3404.0
23	3272.0
24	3988.0
25	4704.0
26	4704.0
27	5800.0
28	6896.0
29	8585.5
30	10275.0
31	12569.0
32	14863.0
33	14863.0
34	18568.0
35	22273.0
36	27287.0
37	32301.0
38	37203.5
39	42106.0
40	42106.0
41	46282.5
42	50459.0
43	54580.0
44	58701.0
45	65445.5
46	72190.0
47	72190.0
48	77093.5
49	81997.0
50	85615.5
51	89234.0
52	92120.5
53	95007.0
54	95007.0
55	94804.5
56	94602.0
57	91832.5
58	89063.0
59	86589.5
60	84116.0
61	84116.0
62	79500.0
63	74884.0
64	70613.5
65	66343.0
66	60548.0
67	54753.0
68	54753.0
69	45798.0
70	36843.0
71	31626.5
72	26410.0
73	20205.0
74	14000.0
75	14000.0
76	10846.0
77	7692.0
78	6320.5
79	4949.0
80	4398.5
81	3848.0
82	3848.0
83	3175.0
84	2502.0
85	2139.5
86	1777.0
87	1485.5
88	1194.0
89	1194.0
90	863.0
91	532.0
92	317.0
93	102.0
94	67.5
95	33.0
96	33.0
97	22.0
98	11.0
99	8.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1161008.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.528408977067144
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.33704180636693	43.11944949285184
2	7.182188197770342	7.258090852144095
3	2.3571656001726606	3.5731148241659447
4	1.2282558563877972	2.482472569601619
5	0.7498857434199095	1.8945266764796613
6	0.48488509833535237	1.4700283533344463
7	0.3493770704394149	1.2357427251660649
8	0.2729174758786915	1.1032068670549904
9	0.20677250405386516	0.9403097085041378
>10	1.4914871096025244	14.946069747912178
>50	0.19688053584128537	7.00200631834293
>100	0.13715733297484692	12.842044010109566
>500	0.005130573219872587	1.6295807663980482
>1k	8.550955366454314E-4	0.503357087934491
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1308	0.1126607224067362	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1278	0.11007676088364594	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	8.6132050769676E-5	0.0
4	0.0	0.0	0.0	8.6132050769676E-5	0.0
5	0.0	0.0	0.0	8.6132050769676E-5	0.0
6	0.0	0.0	0.0	8.6132050769676E-5	0.0
7	0.0	0.0	0.0	8.6132050769676E-5	0.0
8	0.0	0.0	0.0	1.72264101539352E-4	0.0
9	0.0	0.0	0.0	2.58396152309028E-4	0.0
10	0.0	0.0	0.0	2.58396152309028E-4	0.0
11	0.0	0.0	0.0	3.44528203078704E-4	8.6132050769676E-5
12	0.0	0.0	0.0	3.44528203078704E-4	8.6132050769676E-5
13	0.0	8.6132050769676E-5	0.0	4.3066025384838E-4	8.6132050769676E-5
14	0.0	8.6132050769676E-5	0.0	6.029243553877321E-4	8.6132050769676E-5
15	0.0	8.6132050769676E-5	0.0	7.751884569270841E-4	8.6132050769676E-5
16	0.0	8.6132050769676E-5	0.0	0.001033584609236112	8.6132050769676E-5
17	0.0	8.6132050769676E-5	0.0	0.0015503769138541681	8.6132050769676E-5
18	0.0	8.6132050769676E-5	0.0	0.0018949051169328721	8.6132050769676E-5
19	0.0	8.6132050769676E-5	0.0	0.0021533012692419003	8.6132050769676E-5
20	0.0	8.6132050769676E-5	0.0	0.0021533012692419003	8.6132050769676E-5
21	0.0	8.6132050769676E-5	0.0	0.002497829472320604	8.6132050769676E-5
22	0.0	8.6132050769676E-5	0.0	0.003359149980017364	8.6132050769676E-5
23	0.0	8.6132050769676E-5	0.0	0.0057708474015682925	8.6132050769676E-5
24	0.0	8.6132050769676E-5	0.0	0.009732921736973389	8.6132050769676E-5
25	0.0	8.6132050769676E-5	0.0	0.013350467869299781	8.6132050769676E-5
26	0.0	8.6132050769676E-5	0.0	0.021016220387800946	8.6132050769676E-5
27	0.0	8.6132050769676E-5	0.0	0.06287639706186349	8.6132050769676E-5
28	0.0	8.6132050769676E-5	0.0	0.187681738627124	8.6132050769676E-5
29	0.0	8.6132050769676E-5	0.0	0.3539165966125987	8.6132050769676E-5
30	0.0	8.6132050769676E-5	0.0	0.5698496478921764	8.6132050769676E-5
31	0.0	8.6132050769676E-5	0.0	1.008347918360597	8.6132050769676E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	850	0.0	23.07059	1
ACATGTA	145	0.0	21.689655	8
CCCTTAA	60	3.726328E-5	21.583334	1
TAGAACT	285	0.0	20.122808	4
GTTCTAG	235	0.0	18.893616	1
TGTTATG	50	0.007034925	18.499998	2
GTATAGT	50	0.007034925	18.499998	1
TACTAGG	50	0.007034925	18.499998	2
ATATTAC	50	0.007034925	18.499998	1
TCTAGAC	245	0.0	18.122448	3
GTATCAA	1055	0.0	17.36019	2
GACATGT	225	0.0	17.266666	7
GTCTAGA	325	0.0	17.076923	1
CTAGACA	250	0.0	17.02	4
GTAGACC	295	0.0	16.305084	4
GTAGAAT	80	3.382601E-4	16.1875	3
CTAGAAC	315	0.0	15.857142	3
TTAGAAC	105	9.3431845E-6	15.857142	3
ACGGAGT	210	0.0	15.857142	26
CTCTAGT	245	0.0	15.857142	27
>>END_MODULE