FastQCFastQC Report
Fri 10 Feb 2017
ERR1631250.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631250.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences315591
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA5030.15938350586677058No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT4640.1470257390103013No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC4370.13847036195582257No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4350.13783663032215748No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3630.11502229151021416No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC3620.11470542569338163No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC3500.11090303589139107No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT3490.11058617007455854No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC3400.1077343777230656No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG3230.10234765883691234No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2300.025.739131
GCAATAC601.3322751E-624.66666837
CGGACAG502.6960866E-422.234
ATTCGCA450.003819751320.55555714
CGGTGAT450.003819751320.55555714
TGTAGAG450.003819751320.5555575
ATAACGA909.4236384E-820.55555712
ACGGACA555.131737E-420.18181833
GGCAACT656.8818685E-519.92307727
TCGTTTA609.216586E-418.530
CGAACGA801.6106218E-518.516
TGCCCCG500.007023852318.55
TAAAGCC609.216586E-418.54
AAGACGG1002.8585418E-718.55
ATCCGGG701.2156437E-418.515
CGCAGTT500.007023852318.517
AACGAAC801.6106218E-518.514
AGTTCAC609.216586E-418.520
CAGTACC500.007023852318.54
GACGAAA500.007023852318.522