FastQCFastQC Report
Fri 10 Feb 2017
ERR1631249.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631249.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136957
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT27792.029104025351023No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT27111.9794534050833472No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT18111.3223128427170572No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9730.7104419635360003No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA5030.3672685587447155No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4290.3132370013945983No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA3600.26285622494651606No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3000.2190468541220967No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2590.18911045072541016No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2430.17742795183889834No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA2090.15260264170506072No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2040.14895186080302578No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA1990.1453010799009908No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA1990.1453010799009908No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA1930.1409201428185489No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1790.13069795629285105No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT1760.12850748775163007No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA1730.1263170192104091No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA1620.11828530122593223No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1620.11828530122593223No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT1610.11755514504552525No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG1590.11609483268471125No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC1530.11171389560226933No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA1480.10806311470023437No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC1470.1073329585198274No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTG1410.10295202143738545No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA1410.10295202143738545No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT1390.10149170907657147No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1380.1007615528961645No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGACC250.00547966329.632
TATTGAC405.9025857E-527.7530
ATTGACT405.9025857E-527.7531
ATACACA556.194314E-726.9090937
TGACTGT551.8868424E-523.54545433
TTGACTG555.1113707E-420.18181832
CGTCACA555.1113707E-420.18181818
AGTCGGT3600.019.52777711
TATACAC1355.4569682E-1119.18518437
TGGGCAG3700.019.06
ATGCCCC500.00700405718.537
GGGCAGT3550.018.2394377
CAGTCGG3700.018.010
GGCAGTC3650.017.7397258
GCAGTCG3650.017.7397259
AGGGGAA1603.274181E-1117.3437510
GTCGGTG4100.016.69512212
GCCTTAT3050.016.37704826
GGTATCA14050.015.93238451
CTCGCCT3150.015.85714223
Produced by FastQC (version 0.11.5)