FastQCFastQC Report
Fri 10 Feb 2017
ERR1631248.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631248.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2604473
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA57200.21962216540543905No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC53620.20587658232586784No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC35380.13584322049028727No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC33550.12881684701665175No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC32310.12405580706730307No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC32140.12340308384844074No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT26320.10105691247327193No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGA5800.021.6896551
TCAATAC806.967093E-720.81253
ACTATTC4650.020.29032115
ACTGATC4850.019.4536088
AGTGCGC4350.019.13793229
ACGGAGT4900.018.87755226
GCACTAT4900.018.87755213
ATACACA9450.018.79365237
GGTATCA18400.018.7010881
CTAGAAC6950.018.6330933
TAGAACT8050.018.6149064
AACCGAC1306.9849193E-1018.57
TATTCAT5400.018.15740617
CTATTCA5200.018.1442316
GGCACTA5000.018.1312
GGATAGA2350.018.1063841
GTCAAAA6450.018.06976731
TCTAGAC4050.017.8148163
CACTATT5350.017.63551314
GTCGTAC752.0685334E-417.2666661
Produced by FastQC (version 0.11.5)