##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631248.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2604473
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.88265034807426	34.0	33.0	34.0	31.0	34.0
2	33.05664408884254	34.0	33.0	34.0	31.0	34.0
3	33.171930367487015	34.0	33.0	34.0	31.0	34.0
4	36.48601002966819	37.0	37.0	37.0	35.0	37.0
5	36.46291053890749	37.0	37.0	37.0	35.0	37.0
6	36.50726922490654	37.0	37.0	37.0	35.0	37.0
7	36.49631000206184	37.0	37.0	37.0	35.0	37.0
8	36.470982805350644	37.0	37.0	37.0	35.0	37.0
9	38.3383736364324	39.0	39.0	39.0	37.0	39.0
10	38.296392014814515	39.0	39.0	39.0	37.0	39.0
11	38.36118400920263	39.0	39.0	39.0	37.0	39.0
12	38.138058639886076	39.0	39.0	39.0	37.0	39.0
13	38.26514769014691	39.0	39.0	39.0	37.0	39.0
14	39.812319037287004	41.0	40.0	41.0	38.0	41.0
15	39.823976673975885	41.0	40.0	41.0	38.0	41.0
16	39.824092244381106	41.0	40.0	41.0	38.0	41.0
17	39.809954259460554	41.0	40.0	41.0	38.0	41.0
18	39.79552638863985	41.0	40.0	41.0	38.0	41.0
19	39.80153297807272	41.0	40.0	41.0	38.0	41.0
20	39.78048380612892	41.0	40.0	41.0	38.0	41.0
21	39.74897071307708	41.0	40.0	41.0	38.0	41.0
22	39.71204232103769	41.0	40.0	41.0	38.0	41.0
23	39.66027637836906	41.0	40.0	41.0	37.0	41.0
24	39.63112614336951	41.0	40.0	41.0	37.0	41.0
25	39.604820629739685	41.0	40.0	41.0	37.0	41.0
26	39.56259865239532	41.0	40.0	41.0	37.0	41.0
27	39.49862985717264	41.0	39.0	41.0	37.0	41.0
28	39.42305487520892	41.0	39.0	41.0	37.0	41.0
29	39.371849890553676	41.0	39.0	41.0	36.0	41.0
30	39.32825796235937	41.0	39.0	41.0	36.0	41.0
31	39.29693684672485	41.0	39.0	41.0	36.0	41.0
32	39.22771478145483	41.0	39.0	41.0	36.0	41.0
33	39.15511813714329	41.0	39.0	41.0	35.0	41.0
34	39.113379558935726	40.0	39.0	41.0	35.0	41.0
35	39.038734131626626	40.0	39.0	41.0	35.0	41.0
36	38.99218767097989	40.0	39.0	41.0	35.0	41.0
37	38.937072106333986	40.0	39.0	41.0	35.0	41.0
38	38.85631373410283	40.0	38.0	41.0	35.0	41.0
39	38.783641450688876	40.0	38.0	41.0	35.0	41.0
40	38.712582161535174	40.0	38.0	41.0	35.0	41.0
41	38.63671307016813	40.0	38.0	41.0	35.0	41.0
42	38.53731369071593	40.0	38.0	41.0	35.0	41.0
43	37.59613326765146	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	6.0
11	0.0
12	0.0
13	1.0
14	3.0
15	2.0
16	6.0
17	12.0
18	29.0
19	52.0
20	123.0
21	257.0
22	444.0
23	813.0
24	1288.0
25	2084.0
26	3057.0
27	4411.0
28	6449.0
29	8893.0
30	12130.0
31	16715.0
32	22700.0
33	30651.0
34	45898.0
35	69031.0
36	116104.0
37	219481.0
38	563701.0
39	1480131.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.57723309091705	16.418254287911605	11.307201111318873	27.697311509852472
2	20.938247392082772	18.379841142526722	32.375225237504864	28.306686227885642
3	21.093864286556244	19.48647576688259	27.17831975989001	32.24134018667117
4	15.571595482080253	14.136833056053948	31.44958692219117	38.84198453967463
5	17.395803296866582	33.522367096913655	31.87961633697105	17.202213269248713
6	38.42923309245287	31.601287477351462	14.45935511713886	15.510124313056806
7	33.04476567812375	27.183119195322814	18.969902932378258	20.802212194175173
8	29.27283177825226	31.248740148198888	18.20103337604191	21.277394697506942
9	27.930410489953246	13.432544702901508	17.509377136948626	41.12766767019662
10	18.68281990252923	24.17394996991714	30.217859812714508	26.925370314839125
11	39.90838069736181	19.77739834507787	18.75124065405938	21.56298030350094
12	25.057583626322867	22.714806411892155	26.103706968741854	26.123902993043124
13	32.919749983969886	16.787465256886904	23.095113675588113	27.197671083555097
14	24.999952005645675	18.11433637438361	21.49160309974417	35.394108520226546
15	27.80923434414563	24.96896685049144	19.56641516345149	27.655383641911435
16	28.40954772808165	23.040592089071378	20.934983775988464	27.614876406858507
17	27.150483034379704	23.64862296518336	21.92316833386255	27.277725666574387
18	27.982052415210294	21.53291663994981	23.12417905656922	27.360851888270677
19	27.409844525168815	22.67026765107567	22.116911943414273	27.802975880341247
20	27.537048761880044	22.049835033805305	22.705591495861157	27.707524708453494
21	28.92719563612293	21.974887050086526	22.80607247608249	26.29184483770805
22	28.741361496164487	21.785904480484152	21.657817147653287	27.814916875698074
23	27.392835325994934	21.661810277933384	22.760266664311743	28.18508773175994
24	27.036410053012645	22.52359690424896	22.24146689176659	28.19852615097181
25	27.88510381946751	22.217661692019846	22.262277243803258	27.63495724470939
26	28.25316292393893	22.47675441442472	22.064348526554127	27.20573413508222
27	28.4072439990739	21.65697244701711	22.517031276576873	27.418752277332114
28	27.077339638383656	22.18425762140748	22.705706682311547	28.032696057897315
29	26.801391298738746	22.243847411741264	22.695877438545146	28.258883850974843
30	25.86043318552352	22.867351667688627	23.992416124106487	27.27979902268136
31	27.939817383401554	22.182222660783967	22.268842871475343	27.609117084339136
32	26.948791559751246	21.7036613549075	22.976855586523644	28.370691498817614
33	26.82212485980849	21.592084079965506	22.94648475910482	28.639306301121188
34	27.623131435802943	21.33360568529603	23.148790561468672	27.894472317432356
35	26.59831758670564	21.172344654753573	24.24989623620594	27.979441522334845
36	26.008025423953328	21.450174373088146	24.635924426937812	27.905875776020718
37	27.289897034832002	20.243250745928254	24.89325095710341	27.573601262136332
38	25.584484845878606	19.745568489287468	26.181764986621094	28.488181678212825
39	26.391980258578222	19.84044372892328	26.312885562645494	27.454690449853004
40	25.323241976399828	20.509177864389454	26.051143551881704	28.116436607329003
41	24.640570280436773	20.26586568568766	27.843713488294945	27.24985054558062
42	25.03846267555855	20.007118522633945	27.574983499540984	27.379435302266526
43	22.90256032602373	20.015143178677604	27.636416273080965	29.445880222217703
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	16.0
1	19.0
2	22.0
3	59.0
4	96.0
5	96.0
6	125.5
7	155.0
8	150.0
9	145.0
10	198.0
11	251.0
12	251.0
13	432.5
14	614.0
15	945.5
16	1277.0
17	1280.0
18	1283.0
19	1283.0
20	1631.0
21	1979.0
22	2145.0
23	2311.0
24	3170.0
25	4029.0
26	4029.0
27	5285.5
28	6542.0
29	8988.0
30	11434.0
31	15243.5
32	19053.0
33	19053.0
34	25950.5
35	32848.0
36	43456.5
37	54065.0
38	67933.5
39	81802.0
40	81802.0
41	91998.0
42	102194.0
43	114261.5
44	126329.0
45	144913.5
46	163498.0
47	163498.0
48	180472.0
49	197446.0
50	207332.5
51	217219.0
52	228954.5
53	240690.0
54	240690.0
55	237065.0
56	233440.0
57	226654.5
58	219869.0
59	213487.0
60	207105.0
61	207105.0
62	196935.0
63	186765.0
64	176034.0
65	165303.0
66	147614.0
67	129925.0
68	129925.0
69	106095.5
70	82266.0
71	69451.0
72	56636.0
73	40890.5
74	25145.0
75	25145.0
76	18771.5
77	12398.0
78	9775.0
79	7152.0
80	6202.5
81	5253.0
82	5253.0
83	4232.0
84	3211.0
85	2703.5
86	2196.0
87	1792.5
88	1389.0
89	1389.0
90	1120.0
91	851.0
92	511.5
93	172.0
94	115.0
95	58.0
96	58.0
97	46.0
98	34.0
99	20.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2604473.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.48086432718701
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.32155703542509	30.031615534121297
2	8.146016937924845	5.943474774388072
3	2.8242267382642505	3.09090697403496
4	1.4381821603118523	2.098645130724696
5	0.93852201932338	1.7119047227506905
6	0.6519652481451074	1.4270553458173483
7	0.46497194678830883	1.187380495471262
8	0.3885387696871806	1.1339384114248157
9	0.2776095978049029	0.9114694266101052
>10	1.9833790410215486	14.324312335552206
>50	0.2671493540346243	6.871809415293898
>100	0.26124347355779576	18.86535041680883
>500	0.02702952591951883	6.639460977851874
>1k	0.00939698362045772	5.3358197069138695
>5k	2.1116817124624087E-4	0.4268563322362231
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	5720	0.21962216540543905	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	5362	0.20587658232586784	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	3538	0.13584322049028727	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	3355	0.12881684701665175	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	3231	0.12405580706730307	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	3214	0.12340308384844074	No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT	2632	0.10105691247327193	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	3.839548346248934E-5
3	0.0	0.0	0.0	0.0	3.839548346248934E-5
4	0.0	0.0	0.0	0.0	3.839548346248934E-5
5	0.0	3.839548346248934E-5	0.0	0.0	3.839548346248934E-5
6	0.0	3.839548346248934E-5	0.0	0.0	3.839548346248934E-5
7	0.0	3.839548346248934E-5	0.0	0.0	3.839548346248934E-5
8	0.0	3.839548346248934E-5	0.0	0.0	3.839548346248934E-5
9	0.0	3.839548346248934E-5	0.0	3.839548346248934E-5	3.839548346248934E-5
10	0.0	3.839548346248934E-5	0.0	7.679096692497869E-5	3.839548346248934E-5
11	0.0	7.679096692497869E-5	0.0	7.679096692497869E-5	3.839548346248934E-5
12	0.0	7.679096692497869E-5	0.0	7.679096692497869E-5	3.839548346248934E-5
13	0.0	7.679096692497869E-5	0.0	7.679096692497869E-5	3.839548346248934E-5
14	0.0	7.679096692497869E-5	0.0	7.679096692497869E-5	3.839548346248934E-5
15	0.0	7.679096692497869E-5	0.0	1.5358193384995737E-4	3.839548346248934E-5
16	0.0	7.679096692497869E-5	0.0	4.2235031808738276E-4	3.839548346248934E-5
17	0.0	7.679096692497869E-5	0.0	6.527232188623188E-4	3.839548346248934E-5
18	0.0	1.1518645038746802E-4	0.0	8.830961196372548E-4	3.839548346248934E-5
19	0.0	1.1518645038746802E-4	0.0	9.214916030997442E-4	3.839548346248934E-5
20	0.0	1.1518645038746802E-4	0.0	0.0013054464377246376	3.839548346248934E-5
21	0.0	1.1518645038746802E-4	0.0	0.0019965651400494456	3.839548346248934E-5
22	0.0	1.1518645038746802E-4	0.0	0.002802870292761722	3.839548346248934E-5
23	0.0	1.5358193384995737E-4	0.0	0.00410831673048636	3.839548346248934E-5
24	0.0	1.5358193384995737E-4	0.0	0.006757605089398124	3.839548346248934E-5
25	0.0	1.5358193384995737E-4	0.0	0.009099729580609974	3.839548346248934E-5
26	0.0	1.5358193384995737E-4	0.0	0.01508942500075831	3.839548346248934E-5
27	0.0	1.5358193384995737E-4	0.0	0.05002931495162361	3.839548346248934E-5
28	0.0	1.5358193384995737E-4	0.0	0.196316106943708	3.839548346248934E-5
29	0.0	1.5358193384995737E-4	0.0	0.408796712425124	3.839548346248934E-5
30	0.0	1.5358193384995737E-4	0.0	0.6964556745260941	3.839548346248934E-5
31	0.0	1.5358193384995737E-4	0.0	1.3881503091028395	3.839548346248934E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCTAGA	580	0.0	21.689655	1
TCAATAC	80	6.967093E-7	20.8125	3
ACTATTC	465	0.0	20.290321	15
ACTGATC	485	0.0	19.453608	8
AGTGCGC	435	0.0	19.137932	29
ACGGAGT	490	0.0	18.877552	26
GCACTAT	490	0.0	18.877552	13
ATACACA	945	0.0	18.793652	37
GGTATCA	1840	0.0	18.701088	1
CTAGAAC	695	0.0	18.633093	3
TAGAACT	805	0.0	18.614906	4
AACCGAC	130	6.9849193E-10	18.5	7
TATTCAT	540	0.0	18.157406	17
CTATTCA	520	0.0	18.14423	16
GGCACTA	500	0.0	18.13	12
GGATAGA	235	0.0	18.106384	1
GTCAAAA	645	0.0	18.069767	31
TCTAGAC	405	0.0	17.814816	3
CACTATT	535	0.0	17.635513	14
GTCGTAC	75	2.0685334E-4	17.266666	1
>>END_MODULE